Experiment: K. M5a1 KLEB23 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09205 and BWI76_RS09210 are separated by 125 nucleotides BWI76_RS09210 and BWI76_RS09215 are separated by 32 nucleotides BWI76_RS09215 and BWI76_RS09220 are separated by 6 nucleotides BWI76_RS09220 and BWI76_RS09225 overlap by 37 nucleotides BWI76_RS09225 and BWI76_RS09230 are separated by 67 nucleotides BWI76_RS09230 and BWI76_RS09235 overlap by 1 nucleotides BWI76_RS09235 and BWI76_RS09240 overlap by 4 nucleotides
BWI76_RS09205: BWI76_RS09205 - phage repressor protein CI, at 1,942,612 to 1,943,172
_RS09205
BWI76_RS09210: BWI76_RS09210 - hypothetical protein, at 1,943,298 to 1,943,519
_RS09210
BWI76_RS09215: BWI76_RS09215 - phage regulatory CII family protein, at 1,943,552 to 1,944,061
_RS09215
BWI76_RS09220: BWI76_RS09220 - hypothetical protein, at 1,944,068 to 1,944,268
_RS09220
BWI76_RS09225: BWI76_RS09225 - hypothetical protein, at 1,944,232 to 1,944,573
_RS09225
BWI76_RS09230: BWI76_RS09230 - hypothetical protein, at 1,944,641 to 1,944,877
_RS09230
BWI76_RS09235: BWI76_RS09235 - TraR/DksA family transcriptional regulator, at 1,944,877 to 1,945,104
_RS09235
BWI76_RS09240: BWI76_RS09240 - phosphoadenosine phosphosulfate reductase, at 1,945,101 to 1,946,111
_RS09240
Position (kb)
1944
1945 Strain fitness (log2 ratio)
-2
-1
0
1 at 1943.623 kb on - strand, within BWI76_RS09215 at 1943.625 kb on - strand, within BWI76_RS09215 at 1943.651 kb on - strand, within BWI76_RS09215 at 1943.656 kb on - strand, within BWI76_RS09215 at 1943.656 kb on - strand, within BWI76_RS09215 at 1943.666 kb on - strand, within BWI76_RS09215 at 1943.682 kb on + strand, within BWI76_RS09215 at 1943.685 kb on - strand, within BWI76_RS09215 at 1943.685 kb on - strand, within BWI76_RS09215 at 1943.685 kb on - strand at 1943.690 kb on - strand, within BWI76_RS09215 at 1943.818 kb on + strand, within BWI76_RS09215 at 1943.848 kb on - strand, within BWI76_RS09215 at 1943.860 kb on - strand, within BWI76_RS09215 at 1943.967 kb on + strand, within BWI76_RS09215 at 1944.063 kb on - strand at 1944.068 kb on - strand at 1944.078 kb on - strand at 1944.131 kb on + strand, within BWI76_RS09220 at 1944.154 kb on + strand, within BWI76_RS09220 at 1944.155 kb on + strand, within BWI76_RS09220 at 1944.178 kb on - strand, within BWI76_RS09220 at 1944.198 kb on + strand, within BWI76_RS09220 at 1944.206 kb on - strand, within BWI76_RS09220 at 1944.221 kb on - strand, within BWI76_RS09220 at 1944.246 kb on - strand at 1944.259 kb on + strand at 1944.267 kb on - strand at 1944.267 kb on - strand at 1944.269 kb on + strand, within BWI76_RS09225 at 1944.269 kb on + strand, within BWI76_RS09225 at 1944.269 kb on + strand, within BWI76_RS09225 at 1944.274 kb on + strand at 1944.277 kb on - strand, within BWI76_RS09225 at 1944.277 kb on - strand, within BWI76_RS09225 at 1944.290 kb on + strand, within BWI76_RS09225 at 1944.302 kb on - strand, within BWI76_RS09225 at 1944.322 kb on - strand, within BWI76_RS09225 at 1944.469 kb on - strand, within BWI76_RS09225 at 1944.478 kb on - strand, within BWI76_RS09225 at 1944.485 kb on - strand, within BWI76_RS09225 at 1944.485 kb on - strand, within BWI76_RS09225 at 1944.490 kb on - strand, within BWI76_RS09225 at 1944.502 kb on - strand, within BWI76_RS09225 at 1944.504 kb on + strand, within BWI76_RS09225 at 1944.504 kb on + strand, within BWI76_RS09225 at 1944.512 kb on - strand, within BWI76_RS09225 at 1944.512 kb on - strand, within BWI76_RS09225 at 1944.540 kb on - strand at 1944.804 kb on + strand, within BWI76_RS09230 at 1944.812 kb on - strand, within BWI76_RS09230 at 1944.852 kb on - strand, within BWI76_RS09230 at 1944.893 kb on + strand at 1944.896 kb on + strand at 1945.182 kb on - strand at 1945.182 kb on - strand at 1945.218 kb on - strand, within BWI76_RS09240
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 0.1 remove 1,943,623 - BWI76_RS09215 0.14 +0.4 1,943,625 - BWI76_RS09215 0.14 -0.2 1,943,651 - BWI76_RS09215 0.19 -0.8 1,943,656 - BWI76_RS09215 0.20 -0.2 1,943,656 - BWI76_RS09215 0.20 -0.2 1,943,666 - BWI76_RS09215 0.22 -1.1 1,943,682 + BWI76_RS09215 0.25 +0.5 1,943,685 - BWI76_RS09215 0.26 +0.3 1,943,685 - BWI76_RS09215 0.26 -0.0 1,943,685 - -0.7 1,943,690 - BWI76_RS09215 0.27 -0.2 1,943,818 + BWI76_RS09215 0.52 -0.2 1,943,848 - BWI76_RS09215 0.58 +0.5 1,943,860 - BWI76_RS09215 0.60 -0.0 1,943,967 + BWI76_RS09215 0.81 -1.5 1,944,063 - -1.6 1,944,068 - -0.6 1,944,078 - +0.1 1,944,131 + BWI76_RS09220 0.31 -1.1 1,944,154 + BWI76_RS09220 0.43 -0.7 1,944,155 + BWI76_RS09220 0.43 -0.2 1,944,178 - BWI76_RS09220 0.55 -1.8 1,944,198 + BWI76_RS09220 0.65 -1.1 1,944,206 - BWI76_RS09220 0.69 +0.4 1,944,221 - BWI76_RS09220 0.76 -1.1 1,944,246 - -0.6 1,944,259 + +0.7 1,944,267 - +0.8 1,944,267 - +1.3 1,944,269 + BWI76_RS09225 0.11 +0.2 1,944,269 + BWI76_RS09225 0.11 -1.0 1,944,269 + BWI76_RS09225 0.11 -0.7 1,944,274 + +0.3 1,944,277 - BWI76_RS09225 0.13 -0.7 1,944,277 - BWI76_RS09225 0.13 -0.8 1,944,290 + BWI76_RS09225 0.17 -0.1 1,944,302 - BWI76_RS09225 0.20 +0.1 1,944,322 - BWI76_RS09225 0.26 +0.2 1,944,469 - BWI76_RS09225 0.69 -0.2 1,944,478 - BWI76_RS09225 0.72 +0.7 1,944,485 - BWI76_RS09225 0.74 +1.3 1,944,485 - BWI76_RS09225 0.74 +0.3 1,944,490 - BWI76_RS09225 0.75 -0.5 1,944,502 - BWI76_RS09225 0.79 -1.1 1,944,504 + BWI76_RS09225 0.80 -1.6 1,944,504 + BWI76_RS09225 0.80 -0.1 1,944,512 - BWI76_RS09225 0.82 -1.5 1,944,512 - BWI76_RS09225 0.82 -0.1 1,944,540 - -0.7 1,944,804 + BWI76_RS09230 0.69 -0.5 1,944,812 - BWI76_RS09230 0.72 +0.4 1,944,852 - BWI76_RS09230 0.89 -0.5 1,944,893 + -0.9 1,944,896 + +1.3 1,945,182 - -0.2 1,945,182 - +0.0 1,945,218 - BWI76_RS09240 0.12 -0.8
Or see this region's nucleotide sequence