Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27135 and BWI76_RS27140 overlap by 4 nucleotides BWI76_RS27140 and BWI76_RS27145 are separated by 95 nucleotides BWI76_RS27145 and BWI76_RS27150 are separated by 91 nucleotides
BWI76_RS27135: BWI76_RS27135 - selenocysteine-specific translation factor, at 5,631,943 to 5,633,784
_RS27135
BWI76_RS27140: BWI76_RS27140 - L-selenocysteinyl-tRNA(Sec) synthase, at 5,633,781 to 5,635,169
_RS27140
BWI76_RS27145: BWI76_RS27145 - glutathione S-transferase, at 5,635,265 to 5,635,873
_RS27145
BWI76_RS27150: BWI76_RS27150 - secretion protein HlyD, at 5,635,965 to 5,637,101
_RS27150
Position (kb) 5633
5634
5635
5636 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5632.888 kb on - strand, within BWI76_RS27135 at 5632.994 kb on - strand, within BWI76_RS27135 at 5633.113 kb on - strand, within BWI76_RS27135 at 5633.251 kb on + strand, within BWI76_RS27135 at 5633.276 kb on + strand, within BWI76_RS27135 at 5633.278 kb on - strand, within BWI76_RS27135 at 5633.325 kb on + strand, within BWI76_RS27135 at 5633.326 kb on + strand, within BWI76_RS27135 at 5633.356 kb on - strand, within BWI76_RS27135 at 5633.380 kb on - strand, within BWI76_RS27135 at 5633.401 kb on - strand, within BWI76_RS27135 at 5633.414 kb on + strand, within BWI76_RS27135 at 5633.657 kb on + strand at 5633.824 kb on - strand at 5633.829 kb on - strand at 5633.831 kb on + strand at 5633.834 kb on - strand at 5633.834 kb on - strand at 5633.836 kb on + strand at 5633.890 kb on - strand at 5633.936 kb on + strand, within BWI76_RS27140 at 5633.943 kb on + strand, within BWI76_RS27140 at 5633.946 kb on - strand, within BWI76_RS27140 at 5634.120 kb on - strand, within BWI76_RS27140 at 5634.163 kb on + strand, within BWI76_RS27140 at 5634.274 kb on - strand, within BWI76_RS27140 at 5634.360 kb on + strand, within BWI76_RS27140 at 5634.490 kb on - strand, within BWI76_RS27140 at 5634.586 kb on + strand, within BWI76_RS27140 at 5634.904 kb on + strand, within BWI76_RS27140 at 5634.991 kb on - strand, within BWI76_RS27140 at 5635.063 kb on + strand at 5635.288 kb on + strand at 5635.424 kb on - strand, within BWI76_RS27145 at 5635.426 kb on + strand, within BWI76_RS27145 at 5635.434 kb on - strand, within BWI76_RS27145 at 5635.434 kb on - strand, within BWI76_RS27145 at 5635.468 kb on + strand, within BWI76_RS27145 at 5635.476 kb on - strand, within BWI76_RS27145 at 5635.476 kb on - strand, within BWI76_RS27145 at 5635.476 kb on - strand, within BWI76_RS27145 at 5635.617 kb on + strand, within BWI76_RS27145 at 5635.625 kb on - strand, within BWI76_RS27145 at 5635.660 kb on + strand, within BWI76_RS27145 at 5635.668 kb on - strand, within BWI76_RS27145 at 5635.668 kb on - strand, within BWI76_RS27145 at 5635.750 kb on + strand, within BWI76_RS27145 at 5635.758 kb on + strand, within BWI76_RS27145 at 5635.897 kb on + strand at 5635.899 kb on + strand at 5635.944 kb on + strand at 5636.107 kb on + strand, within BWI76_RS27150 at 5636.114 kb on + strand, within BWI76_RS27150 at 5636.114 kb on + strand, within BWI76_RS27150 at 5636.155 kb on - strand, within BWI76_RS27150
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 5,632,888 - BWI76_RS27135 0.51 +0.6 5,632,994 - BWI76_RS27135 0.57 +0.1 5,633,113 - BWI76_RS27135 0.64 +0.1 5,633,251 + BWI76_RS27135 0.71 -0.4 5,633,276 + BWI76_RS27135 0.72 -1.9 5,633,278 - BWI76_RS27135 0.72 -0.1 5,633,325 + BWI76_RS27135 0.75 -1.0 5,633,326 + BWI76_RS27135 0.75 -0.2 5,633,356 - BWI76_RS27135 0.77 +0.2 5,633,380 - BWI76_RS27135 0.78 +0.1 5,633,401 - BWI76_RS27135 0.79 -3.1 5,633,414 + BWI76_RS27135 0.80 +0.5 5,633,657 + +3.4 5,633,824 - -0.1 5,633,829 - -0.5 5,633,831 + -0.2 5,633,834 - -0.2 5,633,834 - +0.0 5,633,836 + -0.4 5,633,890 - -2.0 5,633,936 + BWI76_RS27140 0.11 -1.6 5,633,943 + BWI76_RS27140 0.12 -0.6 5,633,946 - BWI76_RS27140 0.12 +0.4 5,634,120 - BWI76_RS27140 0.24 -1.7 5,634,163 + BWI76_RS27140 0.28 -1.0 5,634,274 - BWI76_RS27140 0.35 -0.8 5,634,360 + BWI76_RS27140 0.42 -2.0 5,634,490 - BWI76_RS27140 0.51 -1.2 5,634,586 + BWI76_RS27140 0.58 -3.7 5,634,904 + BWI76_RS27140 0.81 -3.2 5,634,991 - BWI76_RS27140 0.87 -3.0 5,635,063 + -0.1 5,635,288 + -0.1 5,635,424 - BWI76_RS27145 0.26 -0.3 5,635,426 + BWI76_RS27145 0.26 -0.4 5,635,434 - BWI76_RS27145 0.28 -0.8 5,635,434 - BWI76_RS27145 0.28 -0.8 5,635,468 + BWI76_RS27145 0.33 -0.5 5,635,476 - BWI76_RS27145 0.35 -0.2 5,635,476 - BWI76_RS27145 0.35 -2.1 5,635,476 - BWI76_RS27145 0.35 +0.1 5,635,617 + BWI76_RS27145 0.58 -0.7 5,635,625 - BWI76_RS27145 0.59 +0.2 5,635,660 + BWI76_RS27145 0.65 +0.6 5,635,668 - BWI76_RS27145 0.66 +0.9 5,635,668 - BWI76_RS27145 0.66 -2.7 5,635,750 + BWI76_RS27145 0.80 +0.5 5,635,758 + BWI76_RS27145 0.81 +0.2 5,635,897 + -0.6 5,635,899 + -0.4 5,635,944 + +0.1 5,636,107 + BWI76_RS27150 0.12 +0.2 5,636,114 + BWI76_RS27150 0.13 +0.6 5,636,114 + BWI76_RS27150 0.13 +0.8 5,636,155 - BWI76_RS27150 0.17 +0.1
Or see this region's nucleotide sequence