Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26655 and BWI76_RS26660 are separated by 101 nucleotides BWI76_RS26660 and BWI76_RS26665 are separated by 237 nucleotides BWI76_RS26665 and BWI76_RS26670 overlap by 4 nucleotides
BWI76_RS26655: BWI76_RS26655 - transcription accessory protein, at 5,517,663 to 5,519,993
_RS26655
BWI76_RS26660: BWI76_RS26660 - transcription elongation factor GreB, at 5,520,095 to 5,520,568
_RS26660
BWI76_RS26665: BWI76_RS26665 - DNA-binding response regulator, at 5,520,806 to 5,521,525
_RS26665
BWI76_RS26670: BWI76_RS26670 - two-component sensor histidine kinase, at 5,521,522 to 5,522,883
_RS26670
Position (kb) 5520
5521 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 5519.109 kb on - strand, within BWI76_RS26655 at 5519.109 kb on - strand, within BWI76_RS26655 at 5519.165 kb on - strand, within BWI76_RS26655 at 5519.175 kb on + strand, within BWI76_RS26655 at 5519.193 kb on + strand, within BWI76_RS26655 at 5519.212 kb on - strand, within BWI76_RS26655 at 5519.291 kb on - strand, within BWI76_RS26655 at 5519.352 kb on - strand, within BWI76_RS26655 at 5519.524 kb on + strand at 5519.541 kb on + strand, within BWI76_RS26655 at 5519.542 kb on + strand, within BWI76_RS26655 at 5519.549 kb on - strand, within BWI76_RS26655 at 5519.621 kb on + strand, within BWI76_RS26655 at 5519.819 kb on - strand at 5519.899 kb on - strand at 5519.904 kb on - strand at 5519.926 kb on + strand at 5519.926 kb on + strand at 5519.926 kb on + strand at 5519.934 kb on - strand at 5519.936 kb on + strand at 5519.936 kb on + strand at 5519.944 kb on - strand at 5519.944 kb on - strand at 5519.944 kb on - strand at 5519.944 kb on - strand at 5519.944 kb on - strand at 5519.944 kb on - strand at 5519.944 kb on - strand at 5519.944 kb on - strand at 5519.944 kb on - strand at 5519.949 kb on - strand at 5519.954 kb on - strand at 5520.040 kb on + strand at 5520.177 kb on + strand, within BWI76_RS26660 at 5520.223 kb on - strand, within BWI76_RS26660 at 5520.366 kb on - strand, within BWI76_RS26660 at 5520.467 kb on + strand, within BWI76_RS26660 at 5520.467 kb on + strand, within BWI76_RS26660 at 5520.509 kb on - strand, within BWI76_RS26660 at 5520.541 kb on + strand at 5520.541 kb on + strand at 5520.549 kb on - strand at 5520.549 kb on - strand at 5520.581 kb on - strand at 5520.582 kb on - strand at 5520.595 kb on + strand at 5520.603 kb on - strand at 5520.663 kb on + strand at 5520.664 kb on + strand at 5520.719 kb on + strand at 5520.729 kb on - strand at 5520.788 kb on + strand at 5520.795 kb on - strand at 5520.795 kb on - strand at 5520.796 kb on + strand at 5520.797 kb on - strand at 5520.851 kb on - strand at 5520.862 kb on + strand at 5520.896 kb on - strand, within BWI76_RS26665 at 5520.917 kb on + strand, within BWI76_RS26665 at 5520.953 kb on + strand, within BWI76_RS26665 at 5521.121 kb on - strand, within BWI76_RS26665 at 5521.340 kb on + strand, within BWI76_RS26665 at 5521.512 kb on + strand at 5521.520 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 5,519,109 - BWI76_RS26655 0.62 +0.2 5,519,109 - BWI76_RS26655 0.62 -2.4 5,519,165 - BWI76_RS26655 0.64 -1.0 5,519,175 + BWI76_RS26655 0.65 -0.1 5,519,193 + BWI76_RS26655 0.66 -2.3 5,519,212 - BWI76_RS26655 0.66 +0.4 5,519,291 - BWI76_RS26655 0.70 -0.5 5,519,352 - BWI76_RS26655 0.72 +0.1 5,519,524 + +0.7 5,519,541 + BWI76_RS26655 0.81 -0.4 5,519,542 + BWI76_RS26655 0.81 +0.7 5,519,549 - BWI76_RS26655 0.81 +0.1 5,519,621 + BWI76_RS26655 0.84 +0.7 5,519,819 - -2.6 5,519,899 - -0.2 5,519,904 - -0.5 5,519,926 + +0.6 5,519,926 + -0.7 5,519,926 + -0.8 5,519,934 - -0.5 5,519,936 + -1.9 5,519,936 + +0.1 5,519,944 - -1.4 5,519,944 - +1.1 5,519,944 - -3.4 5,519,944 - +0.2 5,519,944 - -0.5 5,519,944 - +3.3 5,519,944 - -0.6 5,519,944 - -0.6 5,519,944 - +0.0 5,519,949 - +0.0 5,519,954 - +0.2 5,520,040 + -0.7 5,520,177 + BWI76_RS26660 0.17 -1.3 5,520,223 - BWI76_RS26660 0.27 -0.2 5,520,366 - BWI76_RS26660 0.57 -0.9 5,520,467 + BWI76_RS26660 0.78 -1.8 5,520,467 + BWI76_RS26660 0.78 -0.5 5,520,509 - BWI76_RS26660 0.87 -1.6 5,520,541 + -0.3 5,520,541 + +0.1 5,520,549 - +0.3 5,520,549 - +0.0 5,520,581 - -1.4 5,520,582 - +1.4 5,520,595 + -1.2 5,520,603 - -0.1 5,520,663 + +0.7 5,520,664 + +0.2 5,520,719 + +0.4 5,520,729 - +0.6 5,520,788 + -0.6 5,520,795 - +0.4 5,520,795 - +2.3 5,520,796 + +1.8 5,520,797 - +2.6 5,520,851 - +1.2 5,520,862 + +5.5 5,520,896 - BWI76_RS26665 0.12 +1.6 5,520,917 + BWI76_RS26665 0.15 -1.3 5,520,953 + BWI76_RS26665 0.20 +1.6 5,521,121 - BWI76_RS26665 0.44 +0.8 5,521,340 + BWI76_RS26665 0.74 +3.6 5,521,512 + +2.3 5,521,520 - +1.5
Or see this region's nucleotide sequence