Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25620 and BWI76_RS25625 are separated by 53 nucleotides BWI76_RS25625 and BWI76_RS25630 are separated by 21 nucleotides BWI76_RS25630 and BWI76_RS25635 overlap by 2 nucleotides BWI76_RS25635 and BWI76_RS25640 overlap by 25 nucleotides BWI76_RS25640 and BWI76_RS25645 overlap by 4 nucleotides
BWI76_RS25620: BWI76_RS25620 - 16S ribosomal RNA, at 5,323,839 to 5,325,392
_RS25620
BWI76_RS25625: BWI76_RS25625 - hypothetical protein, at 5,325,446 to 5,325,651
_RS25625
BWI76_RS25630: BWI76_RS25630 - hypothetical protein, at 5,325,673 to 5,325,861
_RS25630
BWI76_RS25635: BWI76_RS25635 - gamma carbonic anhydrase family protein, at 5,325,860 to 5,326,414
_RS25635
BWI76_RS25640: BWI76_RS25640 - hypothetical protein, at 5,326,390 to 5,326,647
_RS25640
BWI76_RS25645: BWI76_RS25645 - shikimate dehydrogenase, at 5,326,644 to 5,327,462
_RS25645
Position (kb) 5325
5326
5327 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5325.798 kb on - strand, within BWI76_RS25630 at 5325.807 kb on - strand, within BWI76_RS25630 at 5326.136 kb on - strand, within BWI76_RS25635 at 5326.136 kb on - strand, within BWI76_RS25635 at 5326.264 kb on + strand at 5326.278 kb on + strand, within BWI76_RS25635 at 5326.287 kb on - strand, within BWI76_RS25635 at 5326.342 kb on + strand, within BWI76_RS25635 at 5326.350 kb on - strand, within BWI76_RS25635 at 5326.403 kb on + strand at 5326.403 kb on + strand at 5326.406 kb on - strand at 5326.426 kb on - strand, within BWI76_RS25640 at 5326.426 kb on - strand, within BWI76_RS25640 at 5326.491 kb on + strand, within BWI76_RS25640 at 5326.533 kb on + strand, within BWI76_RS25640 at 5326.548 kb on - strand, within BWI76_RS25640 at 5326.565 kb on - strand, within BWI76_RS25640 at 5326.670 kb on - strand at 5326.891 kb on + strand, within BWI76_RS25645 at 5326.940 kb on - strand, within BWI76_RS25645 at 5327.040 kb on - strand, within BWI76_RS25645 at 5327.065 kb on - strand, within BWI76_RS25645 at 5327.139 kb on + strand, within BWI76_RS25645 at 5327.147 kb on + strand, within BWI76_RS25645 at 5327.147 kb on + strand, within BWI76_RS25645 at 5327.147 kb on - strand, within BWI76_RS25645 at 5327.193 kb on - strand, within BWI76_RS25645 at 5327.217 kb on + strand, within BWI76_RS25645 at 5327.217 kb on + strand, within BWI76_RS25645 at 5327.225 kb on - strand, within BWI76_RS25645 at 5327.301 kb on - strand, within BWI76_RS25645 at 5327.362 kb on - strand, within BWI76_RS25645
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 5,325,798 - BWI76_RS25630 0.66 -0.1 5,325,807 - BWI76_RS25630 0.71 +0.1 5,326,136 - BWI76_RS25635 0.50 -2.7 5,326,136 - BWI76_RS25635 0.50 +0.7 5,326,264 + +1.9 5,326,278 + BWI76_RS25635 0.75 -2.0 5,326,287 - BWI76_RS25635 0.77 -3.1 5,326,342 + BWI76_RS25635 0.87 -1.1 5,326,350 - BWI76_RS25635 0.88 -1.1 5,326,403 + +0.2 5,326,403 + -0.2 5,326,406 - -1.1 5,326,426 - BWI76_RS25640 0.14 +0.2 5,326,426 - BWI76_RS25640 0.14 +0.1 5,326,491 + BWI76_RS25640 0.39 +0.8 5,326,533 + BWI76_RS25640 0.55 +0.2 5,326,548 - BWI76_RS25640 0.61 -0.2 5,326,565 - BWI76_RS25640 0.68 +0.7 5,326,670 - -1.1 5,326,891 + BWI76_RS25645 0.30 +1.4 5,326,940 - BWI76_RS25645 0.36 +0.3 5,327,040 - BWI76_RS25645 0.48 -0.9 5,327,065 - BWI76_RS25645 0.51 +0.8 5,327,139 + BWI76_RS25645 0.60 +0.8 5,327,147 + BWI76_RS25645 0.61 +0.3 5,327,147 + BWI76_RS25645 0.61 -0.6 5,327,147 - BWI76_RS25645 0.61 +1.4 5,327,193 - BWI76_RS25645 0.67 +2.0 5,327,217 + BWI76_RS25645 0.70 +0.8 5,327,217 + BWI76_RS25645 0.70 +1.7 5,327,225 - BWI76_RS25645 0.71 +0.5 5,327,301 - BWI76_RS25645 0.80 +1.3 5,327,362 - BWI76_RS25645 0.88 +0.3
Or see this region's nucleotide sequence