Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23900 and BWI76_RS23905 are separated by 159 nucleotides BWI76_RS23905 and BWI76_RS23915 are separated by 327 nucleotides BWI76_RS23915 and BWI76_RS23920 are separated by 377 nucleotides
BWI76_RS23900: BWI76_RS23900 - hypothetical protein, at 4,989,149 to 4,989,733
_RS23900
BWI76_RS23905: BWI76_RS23905 - Z-ring-associated protein, at 4,989,893 to 4,990,222
_RS23905
BWI76_RS23915: BWI76_RS23915 - 5-formyltetrahydrofolate cyclo-ligase, at 4,990,550 to 4,991,146
_RS23915
BWI76_RS23920: BWI76_RS23920 - D-3-phosphoglycerate dehydrogenase, at 4,991,524 to 4,992,756
_RS23920
Position (kb)
4990
4991
4992 Strain fitness (log2 ratio)
-1
0
1 at 4989.661 kb on - strand, within BWI76_RS23900 at 4989.726 kb on - strand at 4989.726 kb on - strand at 4989.780 kb on - strand at 4989.815 kb on - strand at 4989.828 kb on + strand at 4989.964 kb on - strand, within BWI76_RS23905 at 4989.968 kb on + strand, within BWI76_RS23905 at 4989.969 kb on + strand, within BWI76_RS23905 at 4989.969 kb on + strand, within BWI76_RS23905 at 4989.976 kb on - strand, within BWI76_RS23905 at 4990.133 kb on - strand, within BWI76_RS23905 at 4990.154 kb on - strand, within BWI76_RS23905 at 4990.158 kb on + strand, within BWI76_RS23905 at 4990.239 kb on + strand at 4990.247 kb on - strand at 4990.770 kb on - strand, within BWI76_RS23915 at 4991.082 kb on + strand, within BWI76_RS23915 at 4991.197 kb on + strand at 4991.198 kb on + strand at 4991.205 kb on - strand at 4991.354 kb on - strand at 4991.744 kb on + strand, within BWI76_RS23920 at 4991.783 kb on + strand, within BWI76_RS23920 at 4991.801 kb on - strand, within BWI76_RS23920 at 4991.924 kb on - strand, within BWI76_RS23920
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 4,989,661 - BWI76_RS23900 0.88 +0.9 4,989,726 - -1.3 4,989,726 - -0.8 4,989,780 - +0.4 4,989,815 - +0.5 4,989,828 + -0.5 4,989,964 - BWI76_RS23905 0.22 -0.3 4,989,968 + BWI76_RS23905 0.23 -0.5 4,989,969 + BWI76_RS23905 0.23 -0.6 4,989,969 + BWI76_RS23905 0.23 -1.5 4,989,976 - BWI76_RS23905 0.25 -0.0 4,990,133 - BWI76_RS23905 0.73 +0.7 4,990,154 - BWI76_RS23905 0.79 -0.5 4,990,158 + BWI76_RS23905 0.80 -1.3 4,990,239 + +0.5 4,990,247 - -0.5 4,990,770 - BWI76_RS23915 0.37 -0.6 4,991,082 + BWI76_RS23915 0.89 -1.2 4,991,197 + -0.2 4,991,198 + -1.1 4,991,205 - -0.2 4,991,354 - +1.3 4,991,744 + BWI76_RS23920 0.18 +0.9 4,991,783 + BWI76_RS23920 0.21 +0.3 4,991,801 - BWI76_RS23920 0.22 -0.3 4,991,924 - BWI76_RS23920 0.32 +1.1
Or see this region's nucleotide sequence