Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21325

Experiment: K. M5a1 KLEB23 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21315 and BWI76_RS21320 overlap by 4 nucleotidesBWI76_RS21320 and BWI76_RS21325 overlap by 57 nucleotidesBWI76_RS21325 and BWI76_RS21330 are separated by 50 nucleotides BWI76_RS21315: BWI76_RS21315 - GTPase Era, at 4,463,198 to 4,464,103 _RS21315 BWI76_RS21320: BWI76_RS21320 - ribonuclease 3, at 4,464,100 to 4,464,780 _RS21320 BWI76_RS21325: BWI76_RS21325 - hypothetical protein, at 4,464,724 to 4,464,954 _RS21325 BWI76_RS21330: BWI76_RS21330 - S26 family signal peptidase, at 4,465,005 to 4,465,979 _RS21330 Position (kb) 4464 4465Strain fitness (log2 ratio) -1 0 1 2at 4464.783 kb on - strand, within BWI76_RS21325at 4464.922 kb on + strand, within BWI76_RS21325at 4464.992 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1
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4,464,783 - BWI76_RS21325 0.26 +0.1
4,464,922 + BWI76_RS21325 0.86 +2.4
4,464,992 - +0.4

Or see this region's nucleotide sequence