Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20755 and BWI76_RS20760 are separated by 9 nucleotides BWI76_RS20760 and BWI76_RS20765 are separated by 20 nucleotides BWI76_RS20765 and BWI76_RS20770 are separated by 11 nucleotides
BWI76_RS20755: BWI76_RS20755 - microcompartment protein EutL, at 4,338,114 to 4,338,773
_RS20755
BWI76_RS20760: BWI76_RS20760 - ethanolamine ammonia-lyase small subunit, at 4,338,783 to 4,339,676
_RS20760
BWI76_RS20765: BWI76_RS20765 - ethanolamine ammonia lyase large subunit, at 4,339,697 to 4,341,058
_RS20765
BWI76_RS20770: BWI76_RS20770 - reactivating factor for ethanolamine ammonia lyase, at 4,341,070 to 4,342,473
_RS20770
Position (kb) 4339
4340
4341
4342 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4338.714 kb on + strand at 4339.259 kb on - strand, within BWI76_RS20760 at 4339.278 kb on - strand, within BWI76_RS20760 at 4339.440 kb on - strand, within BWI76_RS20760 at 4339.558 kb on + strand, within BWI76_RS20760 at 4339.843 kb on + strand, within BWI76_RS20765 at 4339.957 kb on + strand, within BWI76_RS20765 at 4340.401 kb on - strand, within BWI76_RS20765 at 4340.450 kb on + strand, within BWI76_RS20765 at 4340.585 kb on + strand, within BWI76_RS20765 at 4340.649 kb on + strand, within BWI76_RS20765 at 4340.746 kb on + strand, within BWI76_RS20765 at 4340.754 kb on - strand, within BWI76_RS20765 at 4341.387 kb on + strand, within BWI76_RS20770 at 4341.463 kb on + strand, within BWI76_RS20770 at 4341.646 kb on - strand, within BWI76_RS20770 at 4341.693 kb on + strand, within BWI76_RS20770 at 4341.907 kb on - strand, within BWI76_RS20770 at 4341.988 kb on - strand, within BWI76_RS20770
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 4,338,714 + -1.0 4,339,259 - BWI76_RS20760 0.53 +1.7 4,339,278 - BWI76_RS20760 0.55 -0.2 4,339,440 - BWI76_RS20760 0.73 -2.6 4,339,558 + BWI76_RS20760 0.87 +0.1 4,339,843 + BWI76_RS20765 0.11 -0.1 4,339,957 + BWI76_RS20765 0.19 -1.3 4,340,401 - BWI76_RS20765 0.52 -2.3 4,340,450 + BWI76_RS20765 0.55 -2.1 4,340,585 + BWI76_RS20765 0.65 -0.8 4,340,649 + BWI76_RS20765 0.70 -1.4 4,340,746 + BWI76_RS20765 0.77 -3.1 4,340,754 - BWI76_RS20765 0.78 -3.2 4,341,387 + BWI76_RS20770 0.23 -0.3 4,341,463 + BWI76_RS20770 0.28 +0.0 4,341,646 - BWI76_RS20770 0.41 +0.5 4,341,693 + BWI76_RS20770 0.44 -0.4 4,341,907 - BWI76_RS20770 0.60 -0.8 4,341,988 - BWI76_RS20770 0.65 -0.8
Or see this region's nucleotide sequence