Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20430

Experiment: K. M5a1 KLEB23 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20420 and BWI76_RS20425 overlap by 1 nucleotidesBWI76_RS20425 and BWI76_RS20430 overlap by 1 nucleotidesBWI76_RS20430 and BWI76_RS20435 are separated by 3 nucleotides BWI76_RS20420: BWI76_RS20420 - elongation factor P hydroxylase, at 4,275,141 to 4,275,689 _RS20420 BWI76_RS20425: BWI76_RS20425 - hypothetical protein, at 4,275,689 to 4,276,498 _RS20425 BWI76_RS20430: BWI76_RS20430 - penicillin-insensitive murein endopeptidase, at 4,276,498 to 4,277,322 _RS20430 BWI76_RS20435: BWI76_RS20435 - chorismate synthase, at 4,277,326 to 4,278,411 _RS20435 Position (kb) 4276 4277 4278Strain fitness (log2 ratio) -2 -1 0 1at 4275.916 kb on + strand, within BWI76_RS20425at 4276.504 kb on + strandat 4277.065 kb on - strand, within BWI76_RS20430at 4277.133 kb on - strand, within BWI76_RS20430at 4277.528 kb on + strand, within BWI76_RS20435at 4278.248 kb on - strand, within BWI76_RS20435

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1
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4,275,916 + BWI76_RS20425 0.28 +1.1
4,276,504 + -2.0
4,277,065 - BWI76_RS20430 0.69 -1.9
4,277,133 - BWI76_RS20430 0.77 -0.8
4,277,528 + BWI76_RS20435 0.19 -2.2
4,278,248 - BWI76_RS20435 0.85 +0.9

Or see this region's nucleotide sequence