Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20360

Experiment: K. M5a1 KLEB23 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20350 and BWI76_RS20355 are separated by 33 nucleotidesBWI76_RS20355 and BWI76_RS20360 are separated by 199 nucleotidesBWI76_RS20360 and BWI76_RS20365 overlap by 11 nucleotides BWI76_RS20350: BWI76_RS20350 - amidophosphoribosyltransferase, at 4,259,839 to 4,261,356 _RS20350 BWI76_RS20355: BWI76_RS20355 - colicin V production protein, at 4,261,390 to 4,261,878 _RS20355 BWI76_RS20360: BWI76_RS20360 - cell division protein DedD, at 4,262,078 to 4,262,767 _RS20360 BWI76_RS20365: BWI76_RS20365 - bifunctional tetrahydrofolate synthase/dihydrofolate synthase, at 4,262,757 to 4,264,025 _RS20365 Position (kb) 4262 4263Strain fitness (log2 ratio) -2 -1 0 1at 4261.323 kb on + strandat 4261.470 kb on + strand, within BWI76_RS20355at 4261.551 kb on - strand, within BWI76_RS20355at 4261.570 kb on + strand, within BWI76_RS20355at 4262.158 kb on + strand, within BWI76_RS20360

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1
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4,261,323 + -2.0
4,261,470 + BWI76_RS20355 0.16 -0.8
4,261,551 - BWI76_RS20355 0.33 -0.0
4,261,570 + BWI76_RS20355 0.37 +0.9
4,262,158 + BWI76_RS20360 0.12 -0.9

Or see this region's nucleotide sequence