Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19325

Experiment: K. M5a1 KLEB23 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19320 and BWI76_RS19325 are separated by 64 nucleotidesBWI76_RS19325 and BWI76_RS19330 are separated by 298 nucleotides BWI76_RS19320: BWI76_RS19320 - putative ABC transport system periplasmic binding protein, at 4,024,237 to 4,025,859 _RS19320 BWI76_RS19325: BWI76_RS19325 - mandelate racemase, at 4,025,924 to 4,027,078 _RS19325 BWI76_RS19330: BWI76_RS19330 - HAD family hydrolase, at 4,027,377 to 4,028,039 _RS19330 Position (kb) 4025 4026 4027 4028Strain fitness (log2 ratio) -3 -2 -1 0 1at 4025.386 kb on - strand, within BWI76_RS19320at 4025.646 kb on + strand, within BWI76_RS19320at 4026.801 kb on - strand, within BWI76_RS19325at 4026.864 kb on - strand, within BWI76_RS19325at 4027.948 kb on - strand, within BWI76_RS19330

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Per-strain Table

Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1
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4,025,386 - BWI76_RS19320 0.71 +0.1
4,025,646 + BWI76_RS19320 0.87 -1.5
4,026,801 - BWI76_RS19325 0.76 -0.4
4,026,864 - BWI76_RS19325 0.81 -3.2
4,027,948 - BWI76_RS19330 0.86 +0.8

Or see this region's nucleotide sequence