Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19005 and BWI76_RS19010 overlap by 4 nucleotides BWI76_RS19010 and BWI76_RS19015 are separated by 16 nucleotides BWI76_RS19015 and BWI76_RS19020 are separated by 224 nucleotides
BWI76_RS19005: BWI76_RS19005 - capsular polysaccharide biosynthesis protein, at 3,945,052 to 3,946,038
_RS19005
BWI76_RS19010: BWI76_RS19010 - polysaccharide pyruvyl transferase yvff, at 3,946,035 to 3,946,982
_RS19010
BWI76_RS19015: BWI76_RS19015 - lipopolysaccharide biosynthesis protein, at 3,946,999 to 3,948,471
_RS19015
BWI76_RS19020: BWI76_RS19020 - UDP-glucose 6-dehydrogenase, at 3,948,696 to 3,949,862
_RS19020
Position (kb)
3946
3947
3948
3949 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 3946.074 kb on - strand at 3946.151 kb on + strand, within BWI76_RS19010 at 3946.241 kb on - strand, within BWI76_RS19010 at 3946.243 kb on - strand, within BWI76_RS19010 at 3946.435 kb on - strand, within BWI76_RS19010 at 3946.435 kb on - strand, within BWI76_RS19010 at 3946.504 kb on - strand, within BWI76_RS19010 at 3946.986 kb on - strand at 3948.560 kb on - strand at 3948.560 kb on - strand at 3948.560 kb on - strand at 3948.560 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 3,946,074 - -0.1 3,946,151 + BWI76_RS19010 0.12 +3.4 3,946,241 - BWI76_RS19010 0.22 +2.3 3,946,243 - BWI76_RS19010 0.22 +2.5 3,946,435 - BWI76_RS19010 0.42 +3.9 3,946,435 - BWI76_RS19010 0.42 +2.0 3,946,504 - BWI76_RS19010 0.49 +3.7 3,946,986 - +0.5 3,948,560 - -0.6 3,948,560 - +0.2 3,948,560 - +1.0 3,948,560 - +0.5
Or see this region's nucleotide sequence