Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18890 and BWI76_RS18895 are separated by 164 nucleotides BWI76_RS18895 and BWI76_RS18900 overlap by 1 nucleotides BWI76_RS18900 and BWI76_RS18905 are separated by 74 nucleotides
BWI76_RS18890: BWI76_RS18890 - nif-specific transcriptional activator NifA, at 3,917,105 to 3,918,679
_RS18890
BWI76_RS18895: BWI76_RS18895 - nitrogenase cofactor biosynthesis protein NifB, at 3,918,844 to 3,920,250
_RS18895
BWI76_RS18900: BWI76_RS18900 - protein NifQ, at 3,920,250 to 3,920,753
_RS18900
BWI76_RS18905: BWI76_RS18905 - putrescine/spermidine ABC transporter, at 3,920,828 to 3,922,186
_RS18905
Position (kb)
3918
3919
3920
3921 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3917.874 kb on - strand, within BWI76_RS18890 at 3918.008 kb on - strand, within BWI76_RS18890 at 3918.214 kb on - strand, within BWI76_RS18890 at 3918.537 kb on + strand at 3918.592 kb on + strand at 3918.600 kb on - strand at 3918.820 kb on - strand at 3918.828 kb on + strand at 3918.879 kb on - strand at 3919.053 kb on + strand, within BWI76_RS18895 at 3919.076 kb on - strand, within BWI76_RS18895 at 3919.088 kb on - strand, within BWI76_RS18895 at 3919.202 kb on + strand, within BWI76_RS18895 at 3919.202 kb on + strand, within BWI76_RS18895 at 3919.202 kb on + strand, within BWI76_RS18895 at 3919.431 kb on + strand, within BWI76_RS18895 at 3919.431 kb on + strand, within BWI76_RS18895 at 3919.550 kb on - strand, within BWI76_RS18895 at 3920.014 kb on - strand, within BWI76_RS18895 at 3920.081 kb on - strand, within BWI76_RS18895 at 3920.334 kb on - strand, within BWI76_RS18900 at 3920.451 kb on + strand, within BWI76_RS18900 at 3920.565 kb on - strand, within BWI76_RS18900 at 3920.846 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 3,917,874 - BWI76_RS18890 0.49 +0.9 3,918,008 - BWI76_RS18890 0.57 +0.1 3,918,214 - BWI76_RS18890 0.70 -1.0 3,918,537 + -0.2 3,918,592 + +1.6 3,918,600 - +0.4 3,918,820 - +0.1 3,918,828 + -0.9 3,918,879 - +2.3 3,919,053 + BWI76_RS18895 0.15 +1.3 3,919,076 - BWI76_RS18895 0.16 -0.8 3,919,088 - BWI76_RS18895 0.17 -1.2 3,919,202 + BWI76_RS18895 0.25 +0.2 3,919,202 + BWI76_RS18895 0.25 +0.0 3,919,202 + BWI76_RS18895 0.25 +0.8 3,919,431 + BWI76_RS18895 0.42 +0.4 3,919,431 + BWI76_RS18895 0.42 +3.0 3,919,550 - BWI76_RS18895 0.50 +0.6 3,920,014 - BWI76_RS18895 0.83 +0.9 3,920,081 - BWI76_RS18895 0.88 -0.0 3,920,334 - BWI76_RS18900 0.17 +0.9 3,920,451 + BWI76_RS18900 0.40 +1.8 3,920,565 - BWI76_RS18900 0.62 +1.4 3,920,846 - +0.3
Or see this region's nucleotide sequence