Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18885 and BWI76_RS18890 overlap by 4 nucleotides BWI76_RS18890 and BWI76_RS18895 are separated by 164 nucleotides
BWI76_RS18885: BWI76_RS18885 - nitrogen fixation regulatory protein, at 3,915,621 to 3,917,108
_RS18885
BWI76_RS18890: BWI76_RS18890 - nif-specific transcriptional activator NifA, at 3,917,105 to 3,918,679
_RS18890
BWI76_RS18895: BWI76_RS18895 - nitrogenase cofactor biosynthesis protein NifB, at 3,918,844 to 3,920,250
_RS18895
Position (kb)
3917
3918
3919 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3916.112 kb on + strand, within BWI76_RS18885 at 3916.779 kb on - strand, within BWI76_RS18885 at 3916.853 kb on - strand, within BWI76_RS18885 at 3917.080 kb on + strand at 3917.098 kb on - strand at 3917.189 kb on + strand at 3917.189 kb on + strand at 3917.231 kb on + strand at 3917.256 kb on + strand at 3917.486 kb on - strand, within BWI76_RS18890 at 3917.499 kb on - strand, within BWI76_RS18890 at 3917.514 kb on + strand, within BWI76_RS18890 at 3917.688 kb on - strand, within BWI76_RS18890 at 3917.688 kb on - strand, within BWI76_RS18890 at 3917.693 kb on - strand, within BWI76_RS18890 at 3917.797 kb on - strand, within BWI76_RS18890 at 3917.799 kb on + strand, within BWI76_RS18890 at 3917.810 kb on - strand, within BWI76_RS18890 at 3917.874 kb on - strand, within BWI76_RS18890 at 3918.008 kb on - strand, within BWI76_RS18890 at 3918.214 kb on - strand, within BWI76_RS18890 at 3918.537 kb on + strand at 3918.592 kb on + strand at 3918.600 kb on - strand at 3918.820 kb on - strand at 3918.828 kb on + strand at 3918.879 kb on - strand at 3919.053 kb on + strand, within BWI76_RS18895 at 3919.076 kb on - strand, within BWI76_RS18895 at 3919.088 kb on - strand, within BWI76_RS18895 at 3919.202 kb on + strand, within BWI76_RS18895 at 3919.202 kb on + strand, within BWI76_RS18895 at 3919.202 kb on + strand, within BWI76_RS18895 at 3919.431 kb on + strand, within BWI76_RS18895 at 3919.431 kb on + strand, within BWI76_RS18895 at 3919.550 kb on - strand, within BWI76_RS18895
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 3,916,112 + BWI76_RS18885 0.33 +1.3 3,916,779 - BWI76_RS18885 0.78 +0.8 3,916,853 - BWI76_RS18885 0.83 +0.9 3,917,080 + +0.7 3,917,098 - +0.4 3,917,189 + -0.0 3,917,189 + +0.1 3,917,231 + +0.4 3,917,256 + -0.0 3,917,486 - BWI76_RS18890 0.24 +0.4 3,917,499 - BWI76_RS18890 0.25 +0.8 3,917,514 + BWI76_RS18890 0.26 +0.5 3,917,688 - BWI76_RS18890 0.37 -0.1 3,917,688 - BWI76_RS18890 0.37 +1.8 3,917,693 - BWI76_RS18890 0.37 +0.9 3,917,797 - BWI76_RS18890 0.44 -0.2 3,917,799 + BWI76_RS18890 0.44 +0.6 3,917,810 - BWI76_RS18890 0.45 -1.4 3,917,874 - BWI76_RS18890 0.49 +0.9 3,918,008 - BWI76_RS18890 0.57 +0.1 3,918,214 - BWI76_RS18890 0.70 -1.0 3,918,537 + -0.2 3,918,592 + +1.6 3,918,600 - +0.4 3,918,820 - +0.1 3,918,828 + -0.9 3,918,879 - +2.3 3,919,053 + BWI76_RS18895 0.15 +1.3 3,919,076 - BWI76_RS18895 0.16 -0.8 3,919,088 - BWI76_RS18895 0.17 -1.2 3,919,202 + BWI76_RS18895 0.25 +0.2 3,919,202 + BWI76_RS18895 0.25 +0.0 3,919,202 + BWI76_RS18895 0.25 +0.8 3,919,431 + BWI76_RS18895 0.42 +0.4 3,919,431 + BWI76_RS18895 0.42 +3.0 3,919,550 - BWI76_RS18895 0.50 +0.6
Or see this region's nucleotide sequence