Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18835 and BWI76_RS18840 are separated by 10 nucleotides BWI76_RS18840 and BWI76_RS18845 overlap by 14 nucleotides BWI76_RS18845 and BWI76_RS18850 are separated by 195 nucleotides
BWI76_RS18835: BWI76_RS18835 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, at 3,906,609 to 3,907,982
_RS18835
BWI76_RS18840: BWI76_RS18840 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifN, at 3,907,993 to 3,909,378
_RS18840
BWI76_RS18845: BWI76_RS18845 - nitrogen fixation protein NifX, at 3,909,365 to 3,909,835
_RS18845
BWI76_RS18850: BWI76_RS18850 - iron-sulfur cluster assembly scaffold protein NifU, at 3,910,031 to 3,910,855
_RS18850
Position (kb)
3907
3908
3909
3910 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3907.024 kb on - strand, within BWI76_RS18835 at 3907.049 kb on + strand, within BWI76_RS18835 at 3907.394 kb on - strand, within BWI76_RS18835 at 3907.405 kb on - strand, within BWI76_RS18835 at 3907.984 kb on - strand at 3908.111 kb on - strand at 3908.286 kb on + strand, within BWI76_RS18840 at 3908.294 kb on - strand, within BWI76_RS18840 at 3908.372 kb on - strand, within BWI76_RS18840 at 3908.437 kb on + strand, within BWI76_RS18840 at 3908.437 kb on + strand, within BWI76_RS18840 at 3908.437 kb on + strand, within BWI76_RS18840 at 3908.445 kb on - strand, within BWI76_RS18840 at 3908.445 kb on - strand, within BWI76_RS18840 at 3908.445 kb on - strand, within BWI76_RS18840 at 3908.445 kb on - strand, within BWI76_RS18840 at 3908.445 kb on - strand, within BWI76_RS18840 at 3908.660 kb on - strand, within BWI76_RS18840 at 3908.681 kb on - strand, within BWI76_RS18840 at 3908.698 kb on + strand, within BWI76_RS18840 at 3908.706 kb on - strand, within BWI76_RS18840 at 3908.716 kb on - strand, within BWI76_RS18840 at 3908.798 kb on + strand, within BWI76_RS18840 at 3908.909 kb on + strand, within BWI76_RS18840 at 3908.917 kb on - strand, within BWI76_RS18840 at 3908.981 kb on - strand, within BWI76_RS18840 at 3909.008 kb on + strand, within BWI76_RS18840 at 3909.394 kb on + strand at 3909.471 kb on - strand at 3909.538 kb on - strand, within BWI76_RS18845 at 3909.610 kb on + strand, within BWI76_RS18845 at 3909.735 kb on + strand, within BWI76_RS18845 at 3910.023 kb on - strand at 3910.273 kb on + strand, within BWI76_RS18850 at 3910.355 kb on - strand, within BWI76_RS18850
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 3,907,024 - BWI76_RS18835 0.30 -1.3 3,907,049 + BWI76_RS18835 0.32 -0.9 3,907,394 - BWI76_RS18835 0.57 +0.8 3,907,405 - BWI76_RS18835 0.58 +0.0 3,907,984 - +0.9 3,908,111 - -0.3 3,908,286 + BWI76_RS18840 0.21 -0.3 3,908,294 - BWI76_RS18840 0.22 -0.4 3,908,372 - BWI76_RS18840 0.27 -2.6 3,908,437 + BWI76_RS18840 0.32 -0.7 3,908,437 + BWI76_RS18840 0.32 +0.6 3,908,437 + BWI76_RS18840 0.32 +0.5 3,908,445 - BWI76_RS18840 0.33 +1.3 3,908,445 - BWI76_RS18840 0.33 -0.2 3,908,445 - BWI76_RS18840 0.33 -0.1 3,908,445 - BWI76_RS18840 0.33 +0.1 3,908,445 - BWI76_RS18840 0.33 -0.7 3,908,660 - BWI76_RS18840 0.48 -0.3 3,908,681 - BWI76_RS18840 0.50 +1.0 3,908,698 + BWI76_RS18840 0.51 +0.4 3,908,706 - BWI76_RS18840 0.51 +0.3 3,908,716 - BWI76_RS18840 0.52 +1.5 3,908,798 + BWI76_RS18840 0.58 -0.3 3,908,909 + BWI76_RS18840 0.66 -1.1 3,908,917 - BWI76_RS18840 0.67 +0.1 3,908,981 - BWI76_RS18840 0.71 +0.4 3,909,008 + BWI76_RS18840 0.73 +2.0 3,909,394 + +0.9 3,909,471 - -1.6 3,909,538 - BWI76_RS18845 0.37 -0.0 3,909,610 + BWI76_RS18845 0.52 +0.7 3,909,735 + BWI76_RS18845 0.79 -0.0 3,910,023 - +0.2 3,910,273 + BWI76_RS18850 0.29 -0.2 3,910,355 - BWI76_RS18850 0.39 +0.4
Or see this region's nucleotide sequence