Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18350 and BWI76_RS18355 overlap by 4 nucleotides BWI76_RS18355 and BWI76_RS18360 are separated by 15 nucleotides BWI76_RS18360 and BWI76_RS18365 are separated by 95 nucleotides
BWI76_RS18350: BWI76_RS18350 - L-cystine ABC transporter ATP-binding protein YecC, at 3,809,827 to 3,810,579
_RS18350
BWI76_RS18355: BWI76_RS18355 - amino acid ABC transporter permease, at 3,810,576 to 3,811,244
_RS18355
BWI76_RS18360: BWI76_RS18360 - aminocyclopropane-1-carboxylate deaminase/D-cysteine desulfhydrase family protein, at 3,811,260 to 3,812,246
_RS18360
BWI76_RS18365: BWI76_RS18365 - cystine ABC transporter substrate-binding protein, at 3,812,342 to 3,813,142
_RS18365
Position (kb)
3811
3812
3813 Strain fitness (log2 ratio)
-2
-1
0
1 at 3810.276 kb on + strand, within BWI76_RS18350 at 3810.324 kb on - strand, within BWI76_RS18350 at 3810.357 kb on + strand, within BWI76_RS18350 at 3810.365 kb on - strand, within BWI76_RS18350 at 3810.437 kb on - strand, within BWI76_RS18350 at 3810.518 kb on - strand at 3810.600 kb on + strand at 3810.724 kb on + strand, within BWI76_RS18355 at 3810.737 kb on - strand, within BWI76_RS18355 at 3811.005 kb on - strand, within BWI76_RS18355 at 3811.173 kb on - strand, within BWI76_RS18355 at 3811.226 kb on + strand at 3811.234 kb on - strand at 3811.286 kb on + strand at 3811.486 kb on + strand, within BWI76_RS18360 at 3811.800 kb on - strand, within BWI76_RS18360 at 3811.968 kb on - strand, within BWI76_RS18360 at 3812.259 kb on - strand at 3812.430 kb on + strand, within BWI76_RS18365 at 3812.451 kb on + strand, within BWI76_RS18365 at 3812.451 kb on + strand, within BWI76_RS18365 at 3812.459 kb on - strand, within BWI76_RS18365 at 3812.777 kb on - strand, within BWI76_RS18365 at 3812.997 kb on - strand, within BWI76_RS18365 at 3813.035 kb on - strand, within BWI76_RS18365 at 3813.130 kb on - strand at 3813.183 kb on - strand at 3813.184 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 3,810,276 + BWI76_RS18350 0.60 -1.8 3,810,324 - BWI76_RS18350 0.66 +0.1 3,810,357 + BWI76_RS18350 0.70 -1.0 3,810,365 - BWI76_RS18350 0.71 -2.4 3,810,437 - BWI76_RS18350 0.81 -0.2 3,810,518 - -0.2 3,810,600 + +0.7 3,810,724 + BWI76_RS18355 0.22 +0.3 3,810,737 - BWI76_RS18355 0.24 +1.0 3,811,005 - BWI76_RS18355 0.64 +0.5 3,811,173 - BWI76_RS18355 0.89 -0.1 3,811,226 + +1.3 3,811,234 - -0.4 3,811,286 + -0.8 3,811,486 + BWI76_RS18360 0.23 -0.2 3,811,800 - BWI76_RS18360 0.55 -0.8 3,811,968 - BWI76_RS18360 0.72 -1.3 3,812,259 - -0.6 3,812,430 + BWI76_RS18365 0.11 -1.8 3,812,451 + BWI76_RS18365 0.14 +0.4 3,812,451 + BWI76_RS18365 0.14 -0.1 3,812,459 - BWI76_RS18365 0.15 -0.9 3,812,777 - BWI76_RS18365 0.54 -0.3 3,812,997 - BWI76_RS18365 0.82 +0.9 3,813,035 - BWI76_RS18365 0.87 +1.2 3,813,130 - -0.8 3,813,183 - -0.0 3,813,184 + -0.9
Or see this region's nucleotide sequence