Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18080 and BWI76_RS18085 are separated by 144 nucleotides BWI76_RS18085 and BWI76_RS18090 are separated by 135 nucleotides BWI76_RS18090 and BWI76_RS18095 are separated by 45 nucleotides BWI76_RS18095 and BWI76_RS18100 are separated by 37 nucleotides
BWI76_RS18080: BWI76_RS18080 - protein YebE, at 3,756,629 to 3,757,309
_RS18080
BWI76_RS18085: BWI76_RS18085 - hypothetical protein, at 3,757,454 to 3,757,816
_RS18085
BWI76_RS18090: BWI76_RS18090 - phosphoribosylglycinamide formyltransferase 2, at 3,757,952 to 3,759,130
_RS18090
BWI76_RS18095: BWI76_RS18095 - ketohydroxyglutarate aldolase, at 3,759,176 to 3,759,817
_RS18095
BWI76_RS18100: BWI76_RS18100 - phosphogluconate dehydratase, at 3,759,855 to 3,761,666
_RS18100
Position (kb) 3757
3758
3759
3760 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3757.080 kb on + strand, within BWI76_RS18080 at 3757.534 kb on - strand, within BWI76_RS18085 at 3757.869 kb on + strand at 3757.992 kb on + strand at 3758.028 kb on - strand at 3758.214 kb on + strand, within BWI76_RS18090 at 3758.441 kb on + strand, within BWI76_RS18090 at 3759.581 kb on - strand, within BWI76_RS18095 at 3759.884 kb on - strand at 3759.884 kb on - strand at 3759.886 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 3,757,080 + BWI76_RS18080 0.66 +0.6 3,757,534 - BWI76_RS18085 0.22 +0.5 3,757,869 + +0.0 3,757,992 + -2.2 3,758,028 - -3.0 3,758,214 + BWI76_RS18090 0.22 -0.0 3,758,441 + BWI76_RS18090 0.41 -1.9 3,759,581 - BWI76_RS18095 0.63 -0.6 3,759,884 - -0.4 3,759,884 - +1.3 3,759,886 + -1.1
Or see this region's nucleotide sequence