Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17295 and BWI76_RS17300 are separated by 35 nucleotides BWI76_RS17300 and BWI76_RS17310 are separated by 282 nucleotides BWI76_RS17310 and BWI76_RS17315 are separated by 107 nucleotides BWI76_RS17315 and BWI76_RS17320 overlap by 4 nucleotides BWI76_RS17320 and BWI76_RS17325 overlap by 1 nucleotides
BWI76_RS17295: BWI76_RS17295 - tRNA-Tyr, at 3,583,462 to 3,583,546
_RS17295
BWI76_RS17300: BWI76_RS17300 - tRNA-Tyr, at 3,583,582 to 3,583,666
_RS17300
BWI76_RS17310: BWI76_RS17310 - AsnC family transcriptional regulator, at 3,583,949 to 3,584,392
_RS17310
BWI76_RS17315: BWI76_RS17315 - EamA family transporter, at 3,584,500 to 3,585,395
_RS17315
BWI76_RS17320: BWI76_RS17320 - respiratory nitrate reductase subunit gamma, at 3,585,392 to 3,586,069
_RS17320
BWI76_RS17325: BWI76_RS17325 - nitrate reductase molybdenum cofactor assembly chaperone, at 3,586,069 to 3,586,779
_RS17325
Position (kb)
3584
3585
3586 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3583.607 kb on - strand, within BWI76_RS17300 at 3583.622 kb on - strand, within BWI76_RS17300 at 3583.819 kb on + strand at 3583.905 kb on + strand at 3583.967 kb on + strand at 3584.037 kb on + strand, within BWI76_RS17310 at 3584.196 kb on + strand, within BWI76_RS17310 at 3584.209 kb on - strand, within BWI76_RS17310 at 3584.262 kb on - strand, within BWI76_RS17310 at 3584.262 kb on - strand, within BWI76_RS17310 at 3584.479 kb on - strand at 3584.626 kb on - strand, within BWI76_RS17315 at 3584.697 kb on + strand, within BWI76_RS17315 at 3584.793 kb on + strand, within BWI76_RS17315 at 3584.798 kb on + strand, within BWI76_RS17315 at 3584.801 kb on - strand, within BWI76_RS17315 at 3584.807 kb on - strand, within BWI76_RS17315 at 3585.328 kb on + strand at 3585.634 kb on + strand, within BWI76_RS17320 at 3585.852 kb on + strand, within BWI76_RS17320 at 3585.976 kb on - strand, within BWI76_RS17320
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 3,583,607 - BWI76_RS17300 0.29 -0.4 3,583,622 - BWI76_RS17300 0.47 -2.3 3,583,819 + +0.6 3,583,905 + -0.3 3,583,967 + +1.1 3,584,037 + BWI76_RS17310 0.20 -0.4 3,584,196 + BWI76_RS17310 0.56 -2.3 3,584,209 - BWI76_RS17310 0.59 +2.0 3,584,262 - BWI76_RS17310 0.70 -1.0 3,584,262 - BWI76_RS17310 0.70 -2.1 3,584,479 - +0.2 3,584,626 - BWI76_RS17315 0.14 -3.4 3,584,697 + BWI76_RS17315 0.22 -2.5 3,584,793 + BWI76_RS17315 0.33 +0.1 3,584,798 + BWI76_RS17315 0.33 +0.1 3,584,801 - BWI76_RS17315 0.34 -1.3 3,584,807 - BWI76_RS17315 0.34 -0.4 3,585,328 + +0.7 3,585,634 + BWI76_RS17320 0.36 -0.6 3,585,852 + BWI76_RS17320 0.68 -0.2 3,585,976 - BWI76_RS17320 0.86 -0.4
Or see this region's nucleotide sequence