Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16700 and BWI76_RS16705 are separated by 74 nucleotides BWI76_RS16705 and BWI76_RS16710 are separated by 496 nucleotides BWI76_RS16710 and BWI76_RS16715 are separated by 66 nucleotides
BWI76_RS16700: BWI76_RS16700 - ABC transporter ATP-binding protein, at 3,456,522 to 3,457,487
_RS16700
BWI76_RS16705: BWI76_RS16705 - glucose dehydrogenase, at 3,457,562 to 3,459,967
_RS16705
BWI76_RS16710: BWI76_RS16710 - cyclic nucleotide-binding protein, at 3,460,464 to 3,460,693
_RS16710
BWI76_RS16715: BWI76_RS16715 - hypothetical protein, at 3,460,760 to 3,461,668
_RS16715
Position (kb)
3457
3458
3459
3460 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3457.530 kb on - strand at 3457.580 kb on + strand at 3457.772 kb on + strand at 3457.901 kb on - strand, within BWI76_RS16705 at 3458.352 kb on + strand, within BWI76_RS16705 at 3458.675 kb on - strand, within BWI76_RS16705 at 3458.739 kb on + strand, within BWI76_RS16705 at 3459.124 kb on + strand, within BWI76_RS16705 at 3459.688 kb on + strand, within BWI76_RS16705 at 3459.771 kb on - strand at 3459.803 kb on - strand at 3459.847 kb on - strand at 3460.060 kb on - strand at 3460.072 kb on + strand at 3460.072 kb on + strand at 3460.072 kb on + strand at 3460.072 kb on + strand at 3460.072 kb on + strand at 3460.072 kb on + strand at 3460.080 kb on - strand at 3460.080 kb on - strand at 3460.080 kb on - strand at 3460.080 kb on - strand at 3460.252 kb on + strand at 3460.258 kb on - strand at 3460.354 kb on + strand at 3460.391 kb on - strand at 3460.416 kb on + strand at 3460.419 kb on + strand at 3460.424 kb on - strand at 3460.424 kb on - strand at 3460.470 kb on - strand at 3460.481 kb on - strand at 3460.520 kb on + strand, within BWI76_RS16710 at 3460.528 kb on - strand at 3460.637 kb on - strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.664 kb on - strand at 3460.693 kb on + strand at 3460.698 kb on + strand at 3460.698 kb on + strand at 3460.698 kb on + strand at 3460.698 kb on + strand at 3460.698 kb on + strand at 3460.725 kb on + strand at 3460.728 kb on - strand at 3460.733 kb on - strand at 3460.812 kb on - strand at 3460.813 kb on - strand at 3460.821 kb on - strand at 3460.830 kb on - strand at 3460.831 kb on - strand at 3460.831 kb on - strand at 3460.863 kb on + strand at 3460.871 kb on - strand, within BWI76_RS16715 at 3460.871 kb on - strand, within BWI76_RS16715 at 3460.871 kb on - strand, within BWI76_RS16715 at 3460.881 kb on - strand, within BWI76_RS16715 at 3460.920 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 3,457,530 - +0.5 3,457,580 + +0.5 3,457,772 + -0.4 3,457,901 - BWI76_RS16705 0.14 -2.5 3,458,352 + BWI76_RS16705 0.33 -0.1 3,458,675 - BWI76_RS16705 0.46 -1.1 3,458,739 + BWI76_RS16705 0.49 +0.2 3,459,124 + BWI76_RS16705 0.65 -1.3 3,459,688 + BWI76_RS16705 0.88 -0.2 3,459,771 - -1.3 3,459,803 - -2.4 3,459,847 - +1.7 3,460,060 - -0.2 3,460,072 + +1.3 3,460,072 + +0.1 3,460,072 + -2.6 3,460,072 + -0.8 3,460,072 + -1.4 3,460,072 + -0.4 3,460,080 - -0.2 3,460,080 - +0.8 3,460,080 - +0.2 3,460,080 - +0.6 3,460,252 + +1.6 3,460,258 - -0.2 3,460,354 + -1.3 3,460,391 - +0.1 3,460,416 + -0.3 3,460,419 + +2.6 3,460,424 - +0.0 3,460,424 - -0.8 3,460,470 - -0.8 3,460,481 - +0.7 3,460,520 + BWI76_RS16710 0.24 -1.2 3,460,528 - +0.1 3,460,637 - BWI76_RS16710 0.75 +0.9 3,460,647 + BWI76_RS16710 0.80 -1.2 3,460,647 + BWI76_RS16710 0.80 +0.5 3,460,647 + BWI76_RS16710 0.80 +0.1 3,460,647 + BWI76_RS16710 0.80 +3.1 3,460,647 + BWI76_RS16710 0.80 -0.9 3,460,647 + BWI76_RS16710 0.80 +1.4 3,460,647 + BWI76_RS16710 0.80 +0.7 3,460,647 + BWI76_RS16710 0.80 +0.3 3,460,655 - BWI76_RS16710 0.83 +0.4 3,460,655 - BWI76_RS16710 0.83 -3.1 3,460,655 - BWI76_RS16710 0.83 +2.1 3,460,655 - BWI76_RS16710 0.83 +3.1 3,460,655 - BWI76_RS16710 0.83 -0.2 3,460,655 - BWI76_RS16710 0.83 +0.2 3,460,655 - BWI76_RS16710 0.83 +0.6 3,460,655 - BWI76_RS16710 0.83 +0.0 3,460,655 - BWI76_RS16710 0.83 -0.9 3,460,655 - BWI76_RS16710 0.83 -0.2 3,460,655 - BWI76_RS16710 0.83 +1.3 3,460,664 - +4.1 3,460,693 + -0.6 3,460,698 + +1.1 3,460,698 + +0.6 3,460,698 + -0.8 3,460,698 + +4.5 3,460,698 + -3.0 3,460,725 + +1.1 3,460,728 - +0.1 3,460,733 - -0.2 3,460,812 - +1.3 3,460,813 - +0.8 3,460,821 - +0.5 3,460,830 - +0.4 3,460,831 - +1.1 3,460,831 - -2.6 3,460,863 + -0.2 3,460,871 - BWI76_RS16715 0.12 +0.3 3,460,871 - BWI76_RS16715 0.12 +0.9 3,460,871 - BWI76_RS16715 0.12 +0.6 3,460,881 - BWI76_RS16715 0.13 -0.9 3,460,920 + +2.1
Or see this region's nucleotide sequence