Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15020 and BWI76_RS15025 are separated by 29 nucleotides BWI76_RS15025 and BWI76_RS15030 overlap by 4 nucleotides BWI76_RS15030 and BWI76_RS15035 overlap by 16 nucleotides BWI76_RS15035 and BWI76_RS15040 are separated by 31 nucleotides
BWI76_RS15020: BWI76_RS15020 - 2-oxobutyrate oxidase, at 3,144,595 to 3,145,623
_RS15020
BWI76_RS15025: BWI76_RS15025 - NLPA lipoprotein, at 3,145,653 to 3,146,459
_RS15025
BWI76_RS15030: BWI76_RS15030 - ABC transporter, at 3,146,456 to 3,147,247
_RS15030
BWI76_RS15035: BWI76_RS15035 - metal ABC transporter permease, at 3,147,232 to 3,147,903
_RS15035
BWI76_RS15040: BWI76_RS15040 - putative 2-nitropropane dioxygenase, at 3,147,935 to 3,148,981
_RS15040
Position (kb) 3146
3147
3148 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3145.681 kb on + strand at 3145.689 kb on - strand at 3146.294 kb on - strand, within BWI76_RS15025 at 3146.888 kb on - strand, within BWI76_RS15030 at 3147.155 kb on - strand, within BWI76_RS15030 at 3147.407 kb on - strand, within BWI76_RS15035 at 3147.752 kb on + strand, within BWI76_RS15035 at 3148.203 kb on + strand, within BWI76_RS15040
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 3,145,681 + +0.3 3,145,689 - -0.6 3,146,294 - BWI76_RS15025 0.79 -0.4 3,146,888 - BWI76_RS15030 0.55 -3.2 3,147,155 - BWI76_RS15030 0.88 +0.3 3,147,407 - BWI76_RS15035 0.26 -0.8 3,147,752 + BWI76_RS15035 0.77 -0.4 3,148,203 + BWI76_RS15040 0.26 -0.1
Or see this region's nucleotide sequence