Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13280 and BWI76_RS13285 are separated by 172 nucleotides BWI76_RS13285 and BWI76_RS13290 overlap by 4 nucleotides BWI76_RS13290 and BWI76_RS13295 are separated by 239 nucleotides
BWI76_RS13280: BWI76_RS13280 - LysR family transcriptional regulator, at 2,768,905 to 2,769,828
_RS13280
BWI76_RS13285: BWI76_RS13285 - protocatechuate 3,4-dioxygenase subunit alpha, at 2,770,001 to 2,770,621
_RS13285
BWI76_RS13290: BWI76_RS13290 - protocatechuate 3,4-dioxygenase subunit beta, at 2,770,618 to 2,771,358
_RS13290
BWI76_RS13295: BWI76_RS13295 - DUF1338 domain-containing protein, at 2,771,598 to 2,772,941
_RS13295
Position (kb)
2770
2771
2772 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2769.700 kb on + strand, within BWI76_RS13280 at 2769.728 kb on - strand, within BWI76_RS13280 at 2769.757 kb on - strand at 2769.783 kb on - strand at 2769.794 kb on + strand at 2770.020 kb on + strand at 2770.256 kb on + strand, within BWI76_RS13285 at 2770.653 kb on + strand at 2770.661 kb on - strand at 2770.888 kb on + strand, within BWI76_RS13290 at 2770.919 kb on - strand, within BWI76_RS13290 at 2771.203 kb on - strand, within BWI76_RS13290 at 2771.475 kb on + strand at 2771.602 kb on - strand at 2771.622 kb on - strand at 2771.689 kb on - strand at 2771.718 kb on - strand at 2771.737 kb on + strand, within BWI76_RS13295 at 2771.759 kb on + strand, within BWI76_RS13295 at 2772.295 kb on + strand, within BWI76_RS13295
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 2,769,700 + BWI76_RS13280 0.86 -0.2 2,769,728 - BWI76_RS13280 0.89 +0.8 2,769,757 - -0.3 2,769,783 - -0.3 2,769,794 + -0.1 2,770,020 + -0.2 2,770,256 + BWI76_RS13285 0.41 +1.2 2,770,653 + +0.7 2,770,661 - -0.8 2,770,888 + BWI76_RS13290 0.36 -2.6 2,770,919 - BWI76_RS13290 0.41 -1.0 2,771,203 - BWI76_RS13290 0.79 -0.9 2,771,475 + -0.5 2,771,602 - +0.5 2,771,622 - -0.7 2,771,689 - -0.5 2,771,718 - -1.2 2,771,737 + BWI76_RS13295 0.10 -2.9 2,771,759 + BWI76_RS13295 0.12 -0.8 2,772,295 + BWI76_RS13295 0.52 +1.4
Or see this region's nucleotide sequence