Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13260 and BWI76_RS13265 are separated by 51 nucleotides BWI76_RS13265 and BWI76_RS13270 are separated by 173 nucleotides BWI76_RS13270 and BWI76_RS13275 are separated by 38 nucleotides BWI76_RS13275 and BWI76_RS13280 are separated by 68 nucleotides
BWI76_RS13260: BWI76_RS13260 - S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase, at 2,764,618 to 2,765,736
_RS13260
BWI76_RS13265: BWI76_RS13265 - hypothetical protein, at 2,765,788 to 2,766,066
_RS13265
BWI76_RS13270: BWI76_RS13270 - membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family, at 2,766,240 to 2,768,612
_RS13270
BWI76_RS13275: BWI76_RS13275 - hypothetical protein, at 2,768,651 to 2,768,836
_RS13275
BWI76_RS13280: BWI76_RS13280 - LysR family transcriptional regulator, at 2,768,905 to 2,769,828
_RS13280
Position (kb)
2766
2767
2768
2769 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2765.249 kb on - strand, within BWI76_RS13260 at 2765.256 kb on - strand, within BWI76_RS13260 at 2765.340 kb on + strand, within BWI76_RS13260 at 2765.572 kb on + strand, within BWI76_RS13260 at 2765.654 kb on - strand at 2765.840 kb on - strand, within BWI76_RS13265 at 2765.883 kb on - strand, within BWI76_RS13265 at 2765.883 kb on - strand, within BWI76_RS13265 at 2765.998 kb on + strand, within BWI76_RS13265 at 2767.015 kb on + strand, within BWI76_RS13270 at 2767.023 kb on - strand, within BWI76_RS13270 at 2767.133 kb on + strand, within BWI76_RS13270 at 2767.297 kb on + strand, within BWI76_RS13270 at 2767.655 kb on + strand, within BWI76_RS13270 at 2767.984 kb on - strand, within BWI76_RS13270 at 2768.034 kb on - strand, within BWI76_RS13270 at 2768.044 kb on - strand, within BWI76_RS13270 at 2768.068 kb on - strand, within BWI76_RS13270 at 2768.281 kb on + strand, within BWI76_RS13270 at 2768.573 kb on - strand at 2768.763 kb on + strand, within BWI76_RS13275 at 2769.007 kb on - strand, within BWI76_RS13280 at 2769.080 kb on - strand, within BWI76_RS13280 at 2769.505 kb on - strand, within BWI76_RS13280 at 2769.507 kb on - strand, within BWI76_RS13280 at 2769.574 kb on - strand, within BWI76_RS13280 at 2769.577 kb on - strand, within BWI76_RS13280
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 2,765,249 - BWI76_RS13260 0.56 -0.8 2,765,256 - BWI76_RS13260 0.57 -0.2 2,765,340 + BWI76_RS13260 0.65 -0.4 2,765,572 + BWI76_RS13260 0.85 -2.6 2,765,654 - -0.3 2,765,840 - BWI76_RS13265 0.19 -2.9 2,765,883 - BWI76_RS13265 0.34 -0.2 2,765,883 - BWI76_RS13265 0.34 +0.5 2,765,998 + BWI76_RS13265 0.75 -0.4 2,767,015 + BWI76_RS13270 0.33 -0.3 2,767,023 - BWI76_RS13270 0.33 +0.1 2,767,133 + BWI76_RS13270 0.38 +0.2 2,767,297 + BWI76_RS13270 0.45 +0.7 2,767,655 + BWI76_RS13270 0.60 +0.7 2,767,984 - BWI76_RS13270 0.73 +0.3 2,768,034 - BWI76_RS13270 0.76 +0.4 2,768,044 - BWI76_RS13270 0.76 -0.6 2,768,068 - BWI76_RS13270 0.77 -3.2 2,768,281 + BWI76_RS13270 0.86 -2.0 2,768,573 - -1.5 2,768,763 + BWI76_RS13275 0.60 -0.4 2,769,007 - BWI76_RS13280 0.11 +1.3 2,769,080 - BWI76_RS13280 0.19 +0.4 2,769,505 - BWI76_RS13280 0.65 -0.6 2,769,507 - BWI76_RS13280 0.65 -0.1 2,769,574 - BWI76_RS13280 0.72 -0.1 2,769,577 - BWI76_RS13280 0.73 +0.3
Or see this region's nucleotide sequence