Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12925 and BWI76_RS12930 overlap by 4 nucleotides BWI76_RS12930 and BWI76_RS12935 overlap by 11 nucleotides BWI76_RS12935 and BWI76_RS12940 are separated by 77 nucleotides BWI76_RS12940 and BWI76_RS12945 are separated by 57 nucleotides
BWI76_RS12925: BWI76_RS12925 - efflux transporter periplasmic adaptor subunit, at 2,692,794 to 2,693,717
_RS12925
BWI76_RS12930: BWI76_RS12930 - DUF1656 domain-containing protein, at 2,693,714 to 2,693,929
_RS12930
BWI76_RS12935: BWI76_RS12935 - fusaric acid resistance protein, at 2,693,919 to 2,695,958
_RS12935
BWI76_RS12940: BWI76_RS12940 - AraC family transcriptional regulator, at 2,696,036 to 2,696,857
_RS12940
BWI76_RS12945: BWI76_RS12945 - AraC family transcriptional regulator, at 2,696,915 to 2,697,310
_RS12945
Position (kb)
2693
2694
2695
2696 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2692.996 kb on - strand, within BWI76_RS12925 at 2693.465 kb on + strand, within BWI76_RS12925 at 2693.734 kb on - strand at 2694.051 kb on + strand at 2694.369 kb on - strand, within BWI76_RS12935 at 2694.511 kb on - strand, within BWI76_RS12935 at 2694.527 kb on + strand, within BWI76_RS12935 at 2694.980 kb on + strand, within BWI76_RS12935 at 2695.368 kb on - strand, within BWI76_RS12935 at 2695.368 kb on - strand, within BWI76_RS12935 at 2695.485 kb on + strand, within BWI76_RS12935 at 2695.493 kb on - strand, within BWI76_RS12935 at 2695.854 kb on + strand at 2695.885 kb on + strand at 2695.885 kb on + strand at 2696.060 kb on - strand at 2696.181 kb on + strand, within BWI76_RS12940 at 2696.414 kb on + strand, within BWI76_RS12940 at 2696.777 kb on - strand at 2696.859 kb on - strand at 2696.859 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 2,692,996 - BWI76_RS12925 0.22 -0.3 2,693,465 + BWI76_RS12925 0.73 +0.4 2,693,734 - -0.3 2,694,051 + +0.3 2,694,369 - BWI76_RS12935 0.22 -0.2 2,694,511 - BWI76_RS12935 0.29 +0.3 2,694,527 + BWI76_RS12935 0.30 -1.8 2,694,980 + BWI76_RS12935 0.52 -4.6 2,695,368 - BWI76_RS12935 0.71 -0.1 2,695,368 - BWI76_RS12935 0.71 -0.5 2,695,485 + BWI76_RS12935 0.77 -0.6 2,695,493 - BWI76_RS12935 0.77 -2.1 2,695,854 + +1.4 2,695,885 + -1.9 2,695,885 + +0.2 2,696,060 - -0.0 2,696,181 + BWI76_RS12940 0.18 -0.9 2,696,414 + BWI76_RS12940 0.46 +0.8 2,696,777 - -1.3 2,696,859 - -1.7 2,696,859 - +0.4
Or see this region's nucleotide sequence