Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12045 and BWI76_RS12050 overlap by 8 nucleotides BWI76_RS12050 and BWI76_RS12055 are separated by 34 nucleotides BWI76_RS12055 and BWI76_RS12060 are separated by 13 nucleotides
BWI76_RS12045: BWI76_RS12045 - hypothetical protein, at 2,531,971 to 2,532,690
_RS12045
BWI76_RS12050: BWI76_RS12050 - hypothetical protein, at 2,532,683 to 2,533,036
_RS12050
BWI76_RS12055: BWI76_RS12055 - DNA single-strand annealing protein RecT-like protein, at 2,533,071 to 2,534,180
_RS12055
BWI76_RS12060: BWI76_RS12060 - putative exodeoxyribonuclease VIII, at 2,534,194 to 2,536,983
_RS12060
Position (kb)
2533
2534
2535 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2532.114 kb on - strand, within BWI76_RS12045 at 2532.233 kb on + strand, within BWI76_RS12045 at 2532.627 kb on + strand at 2532.627 kb on + strand at 2532.642 kb on + strand at 2532.645 kb on - strand at 2532.869 kb on + strand, within BWI76_RS12050 at 2532.877 kb on - strand, within BWI76_RS12050 at 2532.937 kb on + strand, within BWI76_RS12050 at 2532.956 kb on - strand at 2532.989 kb on - strand, within BWI76_RS12050 at 2533.106 kb on - strand at 2533.276 kb on + strand, within BWI76_RS12055 at 2533.531 kb on - strand, within BWI76_RS12055 at 2533.662 kb on - strand, within BWI76_RS12055 at 2533.933 kb on + strand, within BWI76_RS12055 at 2534.271 kb on + strand at 2534.446 kb on + strand at 2534.641 kb on + strand, within BWI76_RS12060 at 2534.723 kb on + strand, within BWI76_RS12060 at 2534.906 kb on - strand, within BWI76_RS12060 at 2534.995 kb on + strand, within BWI76_RS12060
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 2,532,114 - BWI76_RS12045 0.20 -1.3 2,532,233 + BWI76_RS12045 0.36 +0.4 2,532,627 + -0.5 2,532,627 + +0.2 2,532,642 + +1.1 2,532,645 - +0.1 2,532,869 + BWI76_RS12050 0.53 -1.3 2,532,877 - BWI76_RS12050 0.55 +0.7 2,532,937 + BWI76_RS12050 0.72 +0.1 2,532,956 - +2.1 2,532,989 - BWI76_RS12050 0.86 -1.5 2,533,106 - +0.0 2,533,276 + BWI76_RS12055 0.18 -3.2 2,533,531 - BWI76_RS12055 0.41 -0.1 2,533,662 - BWI76_RS12055 0.53 -3.3 2,533,933 + BWI76_RS12055 0.78 -0.5 2,534,271 + +0.7 2,534,446 + -0.4 2,534,641 + BWI76_RS12060 0.16 -2.5 2,534,723 + BWI76_RS12060 0.19 -0.8 2,534,906 - BWI76_RS12060 0.26 +0.2 2,534,995 + BWI76_RS12060 0.29 -0.5
Or see this region's nucleotide sequence