Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10550 and BWI76_RS10555 overlap by 4 nucleotides BWI76_RS10555 and BWI76_RS10560 are separated by 9 nucleotides BWI76_RS10560 and BWI76_RS10565 are separated by 168 nucleotides
BWI76_RS10550: BWI76_RS10550 - AtzE family amidohydrolase, at 2,228,472 to 2,229,869
_RS10550
BWI76_RS10555: BWI76_RS10555 - hypothetical protein, at 2,229,866 to 2,230,054
_RS10555
BWI76_RS10560: BWI76_RS10560 - gamma-glutamyltransferase, at 2,230,064 to 2,231,647
_RS10560
BWI76_RS10565: BWI76_RS10565 - sugar isomerase, at 2,231,816 to 2,232,655
_RS10565
Position (kb)
2230
2231
2232 Strain fitness (log2 ratio)
-2
-1
0
1 at 2229.082 kb on + strand, within BWI76_RS10550 at 2229.087 kb on + strand, within BWI76_RS10550 at 2229.203 kb on + strand, within BWI76_RS10550 at 2230.028 kb on - strand, within BWI76_RS10555 at 2230.180 kb on + strand at 2230.188 kb on - strand at 2230.907 kb on - strand, within BWI76_RS10560 at 2230.999 kb on + strand, within BWI76_RS10560 at 2231.062 kb on + strand, within BWI76_RS10560 at 2231.079 kb on + strand, within BWI76_RS10560 at 2231.594 kb on + strand at 2231.926 kb on - strand, within BWI76_RS10565 at 2232.085 kb on - strand, within BWI76_RS10565 at 2232.153 kb on + strand, within BWI76_RS10565 at 2232.155 kb on - strand, within BWI76_RS10565 at 2232.228 kb on - strand, within BWI76_RS10565 at 2232.320 kb on + strand, within BWI76_RS10565
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 2,229,082 + BWI76_RS10550 0.44 +0.1 2,229,087 + BWI76_RS10550 0.44 +0.8 2,229,203 + BWI76_RS10550 0.52 +0.4 2,230,028 - BWI76_RS10555 0.86 -0.4 2,230,180 + +0.2 2,230,188 - -0.1 2,230,907 - BWI76_RS10560 0.53 -0.3 2,230,999 + BWI76_RS10560 0.59 -1.3 2,231,062 + BWI76_RS10560 0.63 -0.1 2,231,079 + BWI76_RS10560 0.64 -1.9 2,231,594 + -0.6 2,231,926 - BWI76_RS10565 0.13 -0.3 2,232,085 - BWI76_RS10565 0.32 +0.4 2,232,153 + BWI76_RS10565 0.40 +0.1 2,232,155 - BWI76_RS10565 0.40 +1.3 2,232,228 - BWI76_RS10565 0.49 -0.3 2,232,320 + BWI76_RS10565 0.60 +0.5
Or see this region's nucleotide sequence