Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10295 and BWI76_RS10300 are separated by 164 nucleotides BWI76_RS10300 and BWI76_RS10305 are separated by 167 nucleotides BWI76_RS10305 and BWI76_RS10310 are separated by 100 nucleotides
BWI76_RS10295: BWI76_RS10295 - short chain dehydrogenase/reductase family oxidoreductase, at 2,179,777 to 2,180,562
_RS10295
BWI76_RS10300: BWI76_RS10300 - MFS transporter, at 2,180,727 to 2,182,163
_RS10300
BWI76_RS10305: BWI76_RS10305 - transposase, at 2,182,331 to 2,182,927
_RS10305
BWI76_RS10310: BWI76_RS10310 - DNA-binding transcriptional regulator RamA, at 2,183,028 to 2,183,372
_RS10310
Position (kb)
2180
2181
2182
2183 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2180.288 kb on + strand, within BWI76_RS10295 at 2180.309 kb on + strand, within BWI76_RS10295 at 2180.453 kb on - strand, within BWI76_RS10295 at 2180.466 kb on - strand, within BWI76_RS10295 at 2180.807 kb on + strand at 2180.838 kb on + strand at 2181.111 kb on + strand, within BWI76_RS10300 at 2181.390 kb on + strand, within BWI76_RS10300 at 2181.390 kb on + strand, within BWI76_RS10300 at 2181.508 kb on - strand, within BWI76_RS10300 at 2181.516 kb on + strand, within BWI76_RS10300 at 2181.586 kb on - strand, within BWI76_RS10300 at 2181.598 kb on - strand, within BWI76_RS10300 at 2181.844 kb on + strand, within BWI76_RS10300 at 2181.950 kb on - strand, within BWI76_RS10300 at 2182.024 kb on - strand at 2182.246 kb on - strand at 2182.410 kb on + strand, within BWI76_RS10305 at 2182.495 kb on + strand, within BWI76_RS10305 at 2182.622 kb on + strand, within BWI76_RS10305 at 2182.622 kb on + strand, within BWI76_RS10305 at 2182.640 kb on + strand, within BWI76_RS10305 at 2182.651 kb on + strand, within BWI76_RS10305 at 2182.681 kb on + strand, within BWI76_RS10305 at 2182.681 kb on + strand, within BWI76_RS10305 at 2182.681 kb on + strand, within BWI76_RS10305 at 2182.705 kb on + strand, within BWI76_RS10305 at 2182.705 kb on + strand, within BWI76_RS10305 at 2182.711 kb on + strand, within BWI76_RS10305 at 2182.751 kb on + strand, within BWI76_RS10305 at 2182.751 kb on + strand, within BWI76_RS10305 at 2182.772 kb on + strand, within BWI76_RS10305 at 2182.930 kb on - strand at 2182.930 kb on - strand at 2182.943 kb on + strand at 2183.093 kb on + strand, within BWI76_RS10310 at 2183.098 kb on + strand, within BWI76_RS10310 at 2183.098 kb on + strand, within BWI76_RS10310 at 2183.098 kb on + strand, within BWI76_RS10310
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 2,180,288 + BWI76_RS10295 0.65 -1.1 2,180,309 + BWI76_RS10295 0.68 -2.1 2,180,453 - BWI76_RS10295 0.86 +1.3 2,180,466 - BWI76_RS10295 0.88 -1.5 2,180,807 + +1.2 2,180,838 + -0.4 2,181,111 + BWI76_RS10300 0.27 -4.2 2,181,390 + BWI76_RS10300 0.46 -2.4 2,181,390 + BWI76_RS10300 0.46 -0.1 2,181,508 - BWI76_RS10300 0.54 -1.4 2,181,516 + BWI76_RS10300 0.55 +0.6 2,181,586 - BWI76_RS10300 0.60 -0.3 2,181,598 - BWI76_RS10300 0.61 -0.3 2,181,844 + BWI76_RS10300 0.78 -1.4 2,181,950 - BWI76_RS10300 0.85 -0.2 2,182,024 - -0.6 2,182,246 - +0.7 2,182,410 + BWI76_RS10305 0.13 +0.0 2,182,495 + BWI76_RS10305 0.27 -2.4 2,182,622 + BWI76_RS10305 0.49 +0.4 2,182,622 + BWI76_RS10305 0.49 +1.2 2,182,640 + BWI76_RS10305 0.52 +1.6 2,182,651 + BWI76_RS10305 0.54 +0.8 2,182,681 + BWI76_RS10305 0.59 +1.8 2,182,681 + BWI76_RS10305 0.59 +1.1 2,182,681 + BWI76_RS10305 0.59 -0.8 2,182,705 + BWI76_RS10305 0.63 +0.6 2,182,705 + BWI76_RS10305 0.63 +0.1 2,182,711 + BWI76_RS10305 0.64 +1.0 2,182,751 + BWI76_RS10305 0.70 +0.2 2,182,751 + BWI76_RS10305 0.70 -0.7 2,182,772 + BWI76_RS10305 0.74 +1.0 2,182,930 - -1.3 2,182,930 - -0.3 2,182,943 + -0.0 2,183,093 + BWI76_RS10310 0.19 +0.0 2,183,098 + BWI76_RS10310 0.20 -1.2 2,183,098 + BWI76_RS10310 0.20 +2.5 2,183,098 + BWI76_RS10310 0.20 -0.3
Or see this region's nucleotide sequence