Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07750 and BWI76_RS07755 are separated by 13 nucleotides BWI76_RS07755 and BWI76_RS07760 overlap by 1 nucleotides BWI76_RS07760 and BWI76_RS07765 are separated by 0 nucleotides
BWI76_RS07750: BWI76_RS07750 - 2,3-dihydroxybenzoate-AMP ligase, at 1,644,501 to 1,646,108
_RS07750
BWI76_RS07755: BWI76_RS07755 - isochorismatase, at 1,646,122 to 1,646,973
_RS07755
BWI76_RS07760: BWI76_RS07760 - 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase, at 1,646,973 to 1,647,728
_RS07760
BWI76_RS07765: BWI76_RS07765 - thioesterase, at 1,647,729 to 1,648,142
_RS07765
Position (kb) 1646
1647 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1645.289 kb on - strand, within BWI76_RS07750 at 1645.853 kb on + strand, within BWI76_RS07750 at 1645.886 kb on - strand, within BWI76_RS07750 at 1645.886 kb on - strand, within BWI76_RS07750 at 1645.888 kb on + strand, within BWI76_RS07750 at 1645.929 kb on - strand, within BWI76_RS07750 at 1646.004 kb on + strand at 1646.103 kb on + strand at 1646.110 kb on + strand at 1646.283 kb on - strand, within BWI76_RS07755 at 1646.308 kb on - strand, within BWI76_RS07755 at 1646.351 kb on - strand, within BWI76_RS07755 at 1646.351 kb on - strand, within BWI76_RS07755 at 1647.053 kb on - strand, within BWI76_RS07760 at 1647.090 kb on - strand, within BWI76_RS07760 at 1647.138 kb on - strand, within BWI76_RS07760 at 1647.365 kb on - strand, within BWI76_RS07760 at 1647.429 kb on + strand, within BWI76_RS07760 at 1647.815 kb on - strand, within BWI76_RS07765 at 1647.825 kb on - strand, within BWI76_RS07765 at 1647.873 kb on - strand, within BWI76_RS07765
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 1,645,289 - BWI76_RS07750 0.49 +2.2 1,645,853 + BWI76_RS07750 0.84 +0.2 1,645,886 - BWI76_RS07750 0.86 +1.1 1,645,886 - BWI76_RS07750 0.86 -0.4 1,645,888 + BWI76_RS07750 0.86 -0.6 1,645,929 - BWI76_RS07750 0.89 +1.4 1,646,004 + +0.2 1,646,103 + +0.3 1,646,110 + -1.1 1,646,283 - BWI76_RS07755 0.19 +0.0 1,646,308 - BWI76_RS07755 0.22 -1.3 1,646,351 - BWI76_RS07755 0.27 -3.6 1,646,351 - BWI76_RS07755 0.27 -0.7 1,647,053 - BWI76_RS07760 0.11 +0.5 1,647,090 - BWI76_RS07760 0.15 +0.4 1,647,138 - BWI76_RS07760 0.22 +0.9 1,647,365 - BWI76_RS07760 0.52 -1.8 1,647,429 + BWI76_RS07760 0.60 -1.1 1,647,815 - BWI76_RS07765 0.21 -0.7 1,647,825 - BWI76_RS07765 0.23 -0.7 1,647,873 - BWI76_RS07765 0.35 -0.3
Or see this region's nucleotide sequence