Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07090 and BWI76_RS07095 are separated by 137 nucleotides BWI76_RS07095 and BWI76_RS07100 overlap by 1 nucleotides BWI76_RS07100 and BWI76_RS07105 are separated by 340 nucleotides BWI76_RS07105 and BWI76_RS07110 are separated by 363 nucleotides
BWI76_RS07090: BWI76_RS07090 - cysteine--tRNA ligase, at 1,490,052 to 1,491,437
_RS07090
BWI76_RS07095: BWI76_RS07095 - ribosome-associated protein, at 1,491,575 to 1,491,787
_RS07095
BWI76_RS07100: BWI76_RS07100 - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase, at 1,491,787 to 1,492,653
_RS07100
BWI76_RS07105: BWI76_RS07105 - tRNA-Arg, at 1,492,994 to 1,493,070
_RS07105
BWI76_RS07110: BWI76_RS07110 - DNA-binding protein, at 1,493,434 to 1,493,891
_RS07110
Position (kb)
1491
1492
1493 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1491.487 kb on + strand at 1491.487 kb on + strand at 1491.586 kb on + strand at 1491.609 kb on - strand, within BWI76_RS07095 at 1491.674 kb on - strand, within BWI76_RS07095 at 1491.730 kb on - strand, within BWI76_RS07095 at 1491.731 kb on - strand, within BWI76_RS07095 at 1491.803 kb on - strand at 1491.813 kb on - strand at 1491.822 kb on + strand at 1491.822 kb on + strand at 1492.770 kb on - strand at 1492.905 kb on - strand at 1492.951 kb on + strand at 1493.095 kb on - strand at 1493.099 kb on + strand at 1493.145 kb on - strand at 1493.152 kb on + strand at 1493.157 kb on + strand at 1493.157 kb on + strand at 1493.157 kb on + strand at 1493.157 kb on + strand at 1493.157 kb on + strand at 1493.157 kb on + strand at 1493.157 kb on + strand at 1493.159 kb on - strand at 1493.162 kb on + strand at 1493.165 kb on - strand at 1493.165 kb on - strand at 1493.165 kb on - strand at 1493.165 kb on - strand at 1493.165 kb on - strand at 1493.167 kb on + strand at 1493.167 kb on + strand at 1493.167 kb on + strand at 1493.167 kb on + strand at 1493.172 kb on + strand at 1493.175 kb on - strand at 1493.175 kb on - strand at 1493.175 kb on - strand at 1493.175 kb on - strand at 1493.177 kb on + strand at 1493.177 kb on + strand at 1493.180 kb on + strand at 1493.185 kb on - strand at 1493.185 kb on - strand at 1493.187 kb on - strand at 1493.187 kb on - strand at 1493.187 kb on - strand at 1493.188 kb on - strand at 1493.194 kb on - strand at 1493.194 kb on - strand at 1493.215 kb on + strand at 1493.216 kb on - strand at 1493.223 kb on - strand at 1493.225 kb on + strand at 1493.230 kb on - strand at 1493.231 kb on + strand at 1493.233 kb on - strand at 1493.239 kb on - strand at 1493.240 kb on - strand at 1493.295 kb on - strand at 1493.314 kb on + strand at 1493.319 kb on + strand at 1493.321 kb on + strand at 1493.322 kb on - strand at 1493.322 kb on - strand at 1493.327 kb on + strand at 1493.328 kb on + strand at 1493.328 kb on + strand at 1493.328 kb on + strand at 1493.328 kb on + strand at 1493.328 kb on + strand at 1493.328 kb on + strand at 1493.328 kb on + strand at 1493.329 kb on - strand at 1493.329 kb on - strand at 1493.329 kb on - strand at 1493.329 kb on - strand at 1493.329 kb on - strand at 1493.336 kb on - strand at 1493.336 kb on - strand at 1493.336 kb on - strand at 1493.336 kb on - strand at 1493.336 kb on - strand at 1493.336 kb on - strand at 1493.336 kb on - strand at 1493.338 kb on + strand at 1493.436 kb on + strand at 1493.454 kb on - strand at 1493.516 kb on + strand, within BWI76_RS07110 at 1493.576 kb on - strand, within BWI76_RS07110
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 1,491,487 + -1.3 1,491,487 + +0.3 1,491,586 + +1.3 1,491,609 - BWI76_RS07095 0.16 +0.6 1,491,674 - BWI76_RS07095 0.46 +0.5 1,491,730 - BWI76_RS07095 0.73 -0.0 1,491,731 - BWI76_RS07095 0.73 +0.3 1,491,803 - -0.0 1,491,813 - -0.9 1,491,822 + -0.1 1,491,822 + -1.2 1,492,770 - -0.4 1,492,905 - +2.3 1,492,951 + +2.2 1,493,095 - -1.4 1,493,099 + -1.0 1,493,145 - +0.6 1,493,152 + +0.5 1,493,157 + -0.6 1,493,157 + +1.2 1,493,157 + -3.2 1,493,157 + +0.6 1,493,157 + -1.9 1,493,157 + -0.1 1,493,157 + +0.1 1,493,159 - -0.5 1,493,162 + -1.9 1,493,165 - +2.3 1,493,165 - -3.4 1,493,165 - -3.2 1,493,165 - -1.6 1,493,165 - -0.4 1,493,167 + +1.3 1,493,167 + +3.4 1,493,167 + +0.3 1,493,167 + -1.2 1,493,172 + +0.2 1,493,175 - -0.5 1,493,175 - -1.4 1,493,175 - -2.6 1,493,175 - -0.4 1,493,177 + +0.7 1,493,177 + +0.0 1,493,180 + -2.4 1,493,185 - -1.0 1,493,185 - -1.1 1,493,187 - -3.3 1,493,187 - +1.0 1,493,187 - -0.8 1,493,188 - +0.8 1,493,194 - +0.1 1,493,194 - +1.6 1,493,215 + -0.1 1,493,216 - +0.4 1,493,223 - -1.6 1,493,225 + +2.0 1,493,230 - +2.3 1,493,231 + +1.8 1,493,233 - -1.3 1,493,239 - -1.7 1,493,240 - +0.6 1,493,295 - +0.5 1,493,314 + -0.4 1,493,319 + -0.7 1,493,321 + +0.4 1,493,322 - +1.0 1,493,322 - +1.6 1,493,327 + +1.0 1,493,328 + +0.4 1,493,328 + -0.8 1,493,328 + -2.4 1,493,328 + -0.8 1,493,328 + +1.7 1,493,328 + -2.8 1,493,328 + +1.0 1,493,329 - -2.1 1,493,329 - -1.6 1,493,329 - -1.2 1,493,329 - +1.0 1,493,329 - -0.8 1,493,336 - -0.6 1,493,336 - -2.3 1,493,336 - -0.2 1,493,336 - -0.5 1,493,336 - -0.3 1,493,336 - -0.6 1,493,336 - +0.3 1,493,338 + -1.6 1,493,436 + -1.5 1,493,454 - -0.6 1,493,516 + BWI76_RS07110 0.18 +2.0 1,493,576 - BWI76_RS07110 0.31 -0.0
Or see this region's nucleotide sequence