Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06545 and BWI76_RS06550 are separated by 148 nucleotides BWI76_RS06550 and BWI76_RS06555 are separated by 110 nucleotides BWI76_RS06555 and BWI76_RS06560 are separated by 201 nucleotides
BWI76_RS06545: BWI76_RS06545 - peptidylprolyl isomerase, at 1,375,750 to 1,377,624
_RS06545
BWI76_RS06550: BWI76_RS06550 - competence protein ComEA, at 1,377,773 to 1,378,138
_RS06550
BWI76_RS06555: BWI76_RS06555 - thioesterase superfamily protein, at 1,378,249 to 1,378,650
_RS06555
BWI76_RS06560: BWI76_RS06560 - 7-cyano-7-deazaguanine synthase QueC, at 1,378,852 to 1,379,547
_RS06560
Position (kb)
1377
1378
1379 Strain fitness (log2 ratio)
-1
0
1 at 1376.829 kb on + strand at 1377.238 kb on - strand, within BWI76_RS06545 at 1377.660 kb on - strand at 1377.806 kb on + strand at 1377.819 kb on - strand, within BWI76_RS06550 at 1377.918 kb on + strand, within BWI76_RS06550 at 1378.025 kb on + strand, within BWI76_RS06550 at 1378.079 kb on - strand, within BWI76_RS06550 at 1378.745 kb on + strand at 1378.987 kb on + strand, within BWI76_RS06560 at 1378.992 kb on + strand, within BWI76_RS06560 at 1379.119 kb on + strand, within BWI76_RS06560
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 1,376,829 + -1.5 1,377,238 - BWI76_RS06545 0.79 +1.2 1,377,660 - +0.4 1,377,806 + +0.5 1,377,819 - BWI76_RS06550 0.13 +0.7 1,377,918 + BWI76_RS06550 0.40 +0.6 1,378,025 + BWI76_RS06550 0.69 +0.3 1,378,079 - BWI76_RS06550 0.84 +1.2 1,378,745 + +1.3 1,378,987 + BWI76_RS06560 0.19 -1.1 1,378,992 + BWI76_RS06560 0.20 +0.3 1,379,119 + BWI76_RS06560 0.38 -0.4
Or see this region's nucleotide sequence