Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04610 and BWI76_RS04615 overlap by 25 nucleotides BWI76_RS04615 and BWI76_RS04620 are separated by 162 nucleotides
BWI76_RS04610: BWI76_RS04610 - thiamine/thiamine pyrophosphate ABC transporter permease ThiP, at 959,191 to 960,801
_RS04610
BWI76_RS04615: BWI76_RS04615 - thiamine transporter substrate binding subunit, at 960,777 to 961,760
_RS04615
BWI76_RS04620: BWI76_RS04620 - transcriptional regulator SgrR, at 961,923 to 963,578
_RS04620
Position (kb) 960
961
962 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 959.799 kb on + strand, within BWI76_RS04610 at 959.944 kb on + strand, within BWI76_RS04610 at 959.944 kb on + strand, within BWI76_RS04610 at 960.041 kb on - strand at 960.227 kb on + strand, within BWI76_RS04610 at 960.236 kb on - strand, within BWI76_RS04610 at 960.254 kb on - strand, within BWI76_RS04610 at 960.445 kb on + strand, within BWI76_RS04610 at 960.448 kb on - strand, within BWI76_RS04610 at 960.506 kb on - strand, within BWI76_RS04610 at 960.555 kb on + strand, within BWI76_RS04610 at 960.909 kb on - strand, within BWI76_RS04615 at 961.024 kb on + strand, within BWI76_RS04615 at 961.132 kb on - strand, within BWI76_RS04615 at 961.132 kb on - strand, within BWI76_RS04615 at 961.321 kb on + strand, within BWI76_RS04615 at 961.329 kb on - strand, within BWI76_RS04615 at 961.495 kb on + strand, within BWI76_RS04615 at 961.674 kb on - strand at 962.083 kb on + strand at 962.166 kb on - strand, within BWI76_RS04620 at 962.225 kb on - strand, within BWI76_RS04620 at 962.234 kb on + strand, within BWI76_RS04620 at 962.615 kb on + strand, within BWI76_RS04620 at 962.759 kb on + strand, within BWI76_RS04620
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 959,799 + BWI76_RS04610 0.38 +1.0 959,944 + BWI76_RS04610 0.47 -2.3 959,944 + BWI76_RS04610 0.47 +1.4 960,041 - -0.6 960,227 + BWI76_RS04610 0.64 -0.5 960,236 - BWI76_RS04610 0.65 +1.4 960,254 - BWI76_RS04610 0.66 +1.0 960,445 + BWI76_RS04610 0.78 +1.6 960,448 - BWI76_RS04610 0.78 +1.1 960,506 - BWI76_RS04610 0.82 +0.2 960,555 + BWI76_RS04610 0.85 -0.0 960,909 - BWI76_RS04615 0.13 +0.6 961,024 + BWI76_RS04615 0.25 +0.8 961,132 - BWI76_RS04615 0.36 +0.8 961,132 - BWI76_RS04615 0.36 +0.8 961,321 + BWI76_RS04615 0.55 +0.9 961,329 - BWI76_RS04615 0.56 +0.3 961,495 + BWI76_RS04615 0.73 +0.1 961,674 - -0.2 962,083 + -3.5 962,166 - BWI76_RS04620 0.15 +1.0 962,225 - BWI76_RS04620 0.18 +0.3 962,234 + BWI76_RS04620 0.19 +2.2 962,615 + BWI76_RS04620 0.42 -2.1 962,759 + BWI76_RS04620 0.50 -0.7
Or see this region's nucleotide sequence