Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04345 and BWI76_RS04350 are separated by 54 nucleotides BWI76_RS04350 and BWI76_RS04355 are separated by 1 nucleotides BWI76_RS04355 and BWI76_RS04360 are separated by 65 nucleotides
BWI76_RS04345: BWI76_RS04345 - signal peptidase II, at 895,864 to 896,364
_RS04345
BWI76_RS04350: BWI76_RS04350 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 896,419 to 896,868
_RS04350
BWI76_RS04355: BWI76_RS04355 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 896,870 to 897,820
_RS04355
BWI76_RS04360: BWI76_RS04360 - ribonucleoside hydrolase RihC, at 897,886 to 898,800
_RS04360
Position (kb) 896
897
898 Strain fitness (log2 ratio) -2
-1
0
1
2 at 896.421 kb on + strand at 896.558 kb on + strand, within BWI76_RS04350 at 897.853 kb on + strand at 897.853 kb on + strand at 898.063 kb on - strand, within BWI76_RS04360 at 898.115 kb on - strand, within BWI76_RS04360 at 898.350 kb on + strand, within BWI76_RS04360 at 898.453 kb on - strand at 898.558 kb on + strand, within BWI76_RS04360 at 898.628 kb on + strand, within BWI76_RS04360
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 896,421 + -1.2 896,558 + BWI76_RS04350 0.31 -2.6 897,853 + +0.1 897,853 + +0.4 898,063 - BWI76_RS04360 0.19 +0.6 898,115 - BWI76_RS04360 0.25 -0.5 898,350 + BWI76_RS04360 0.51 +2.6 898,453 - +2.0 898,558 + BWI76_RS04360 0.73 -0.3 898,628 + BWI76_RS04360 0.81 +1.5
Or see this region's nucleotide sequence