Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03535 and BWI76_RS03540 are separated by 123 nucleotides BWI76_RS03540 and BWI76_RS03545 are separated by 241 nucleotides BWI76_RS03545 and BWI76_RS03550 are separated by 17 nucleotides
BWI76_RS03535: BWI76_RS03535 - Tryptophanase (EC 4.1.99.1) <small>(from data)</small>, at 734,065 to 735,453
_RS03535
BWI76_RS03540: BWI76_RS03540 - tryptophanase leader peptide, at 735,577 to 735,672
_RS03540
BWI76_RS03545: BWI76_RS03545 - QacE family quaternary ammonium compound efflux SMR transporter, at 735,914 to 736,243
_RS03545
BWI76_RS03550: BWI76_RS03550 - QacE family quaternary ammonium compound efflux SMR transporter, at 736,261 to 736,680
_RS03550
Position (kb) 735
736 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 734.609 kb on + strand, within BWI76_RS03535 at 734.639 kb on - strand, within BWI76_RS03535 at 734.644 kb on - strand, within BWI76_RS03535 at 734.686 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.691 kb on + strand, within BWI76_RS03535 at 734.694 kb on - strand, within BWI76_RS03535 at 734.694 kb on - strand, within BWI76_RS03535 at 734.699 kb on - strand, within BWI76_RS03535 at 734.699 kb on - strand, within BWI76_RS03535 at 734.699 kb on - strand, within BWI76_RS03535 at 734.706 kb on + strand, within BWI76_RS03535 at 734.744 kb on + strand, within BWI76_RS03535 at 734.752 kb on - strand, within BWI76_RS03535 at 734.752 kb on - strand, within BWI76_RS03535 at 734.763 kb on + strand, within BWI76_RS03535 at 734.836 kb on + strand, within BWI76_RS03535 at 734.842 kb on - strand, within BWI76_RS03535 at 734.844 kb on - strand, within BWI76_RS03535 at 734.885 kb on + strand, within BWI76_RS03535 at 734.887 kb on + strand, within BWI76_RS03535 at 734.893 kb on + strand, within BWI76_RS03535 at 734.940 kb on - strand, within BWI76_RS03535 at 734.942 kb on - strand, within BWI76_RS03535 at 734.989 kb on + strand, within BWI76_RS03535 at 734.989 kb on + strand, within BWI76_RS03535 at 735.009 kb on - strand, within BWI76_RS03535 at 735.047 kb on + strand, within BWI76_RS03535 at 735.144 kb on + strand, within BWI76_RS03535 at 735.157 kb on - strand, within BWI76_RS03535 at 735.159 kb on + strand, within BWI76_RS03535 at 735.174 kb on - strand, within BWI76_RS03535 at 735.176 kb on + strand, within BWI76_RS03535 at 735.176 kb on + strand, within BWI76_RS03535 at 735.176 kb on + strand, within BWI76_RS03535 at 735.183 kb on - strand, within BWI76_RS03535 at 735.232 kb on - strand, within BWI76_RS03535 at 735.238 kb on + strand, within BWI76_RS03535 at 735.266 kb on - strand, within BWI76_RS03535 at 735.284 kb on - strand, within BWI76_RS03535 at 735.294 kb on - strand, within BWI76_RS03535 at 735.299 kb on - strand, within BWI76_RS03535 at 735.299 kb on - strand, within BWI76_RS03535 at 735.315 kb on + strand at 735.320 kb on + strand at 735.320 kb on + strand at 735.327 kb on - strand at 735.362 kb on + strand at 735.362 kb on + strand at 735.366 kb on + strand at 735.374 kb on + strand at 735.375 kb on - strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.387 kb on + strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.395 kb on - strand at 735.409 kb on + strand at 735.433 kb on + strand at 735.435 kb on + strand at 735.435 kb on + strand at 735.435 kb on + strand at 735.435 kb on + strand at 735.435 kb on + strand at 735.435 kb on + strand at 735.443 kb on - strand at 735.443 kb on - strand at 735.443 kb on - strand at 735.443 kb on - strand at 735.443 kb on - strand at 735.443 kb on - strand at 735.468 kb on + strand at 735.481 kb on + strand at 735.596 kb on - strand, within BWI76_RS03540 at 735.611 kb on + strand, within BWI76_RS03540 at 735.612 kb on + strand, within BWI76_RS03540 at 735.614 kb on - strand, within BWI76_RS03540 at 735.634 kb on + strand, within BWI76_RS03540 at 735.634 kb on + strand, within BWI76_RS03540 at 735.634 kb on + strand, within BWI76_RS03540 at 735.634 kb on + strand, within BWI76_RS03540 at 735.639 kb on + strand, within BWI76_RS03540 at 735.642 kb on - strand, within BWI76_RS03540 at 735.674 kb on + strand at 735.682 kb on - strand at 735.688 kb on + strand at 735.697 kb on - strand at 735.770 kb on + strand at 735.803 kb on + strand at 735.806 kb on + strand at 735.827 kb on - strand at 735.880 kb on + strand at 735.912 kb on + strand at 735.912 kb on + strand at 735.913 kb on + strand at 735.914 kb on - strand at 735.920 kb on - strand at 735.964 kb on - strand, within BWI76_RS03545 at 735.964 kb on - strand, within BWI76_RS03545 at 735.974 kb on - strand, within BWI76_RS03545 at 736.036 kb on + strand, within BWI76_RS03545 at 736.038 kb on + strand, within BWI76_RS03545 at 736.075 kb on + strand, within BWI76_RS03545 at 736.075 kb on + strand, within BWI76_RS03545 at 736.094 kb on + strand, within BWI76_RS03545 at 736.102 kb on - strand, within BWI76_RS03545 at 736.104 kb on + strand, within BWI76_RS03545 at 736.112 kb on - strand, within BWI76_RS03545 at 736.149 kb on + strand, within BWI76_RS03545 at 736.457 kb on - strand, within BWI76_RS03550 at 736.478 kb on + strand, within BWI76_RS03550 at 736.478 kb on + strand, within BWI76_RS03550 at 736.478 kb on + strand, within BWI76_RS03550 at 736.491 kb on - strand, within BWI76_RS03550 at 736.491 kb on - strand, within BWI76_RS03550 at 736.509 kb on - strand, within BWI76_RS03550 at 736.548 kb on - strand, within BWI76_RS03550 at 736.608 kb on + strand, within BWI76_RS03550
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 734,609 + BWI76_RS03535 0.39 -1.1 734,639 - BWI76_RS03535 0.41 -0.3 734,644 - BWI76_RS03535 0.42 -3.9 734,686 + BWI76_RS03535 0.45 -0.5 734,691 + BWI76_RS03535 0.45 -1.1 734,691 + BWI76_RS03535 0.45 +1.7 734,691 + BWI76_RS03535 0.45 -3.2 734,691 + BWI76_RS03535 0.45 +0.7 734,691 + BWI76_RS03535 0.45 +0.0 734,691 + BWI76_RS03535 0.45 -0.6 734,694 - BWI76_RS03535 0.45 -0.2 734,694 - BWI76_RS03535 0.45 -1.3 734,699 - BWI76_RS03535 0.46 -0.9 734,699 - BWI76_RS03535 0.46 +1.5 734,699 - BWI76_RS03535 0.46 -1.1 734,706 + BWI76_RS03535 0.46 +1.4 734,744 + BWI76_RS03535 0.49 +1.0 734,752 - BWI76_RS03535 0.49 -0.6 734,752 - BWI76_RS03535 0.49 -0.6 734,763 + BWI76_RS03535 0.50 -2.4 734,836 + BWI76_RS03535 0.56 -0.5 734,842 - BWI76_RS03535 0.56 -0.2 734,844 - BWI76_RS03535 0.56 -0.8 734,885 + BWI76_RS03535 0.59 +0.2 734,887 + BWI76_RS03535 0.59 -1.3 734,893 + BWI76_RS03535 0.60 +0.2 734,940 - BWI76_RS03535 0.63 +0.2 734,942 - BWI76_RS03535 0.63 -1.3 734,989 + BWI76_RS03535 0.67 +0.0 734,989 + BWI76_RS03535 0.67 -1.6 735,009 - BWI76_RS03535 0.68 -0.2 735,047 + BWI76_RS03535 0.71 -0.7 735,144 + BWI76_RS03535 0.78 +0.7 735,157 - BWI76_RS03535 0.79 +0.9 735,159 + BWI76_RS03535 0.79 +0.0 735,174 - BWI76_RS03535 0.80 +0.0 735,176 + BWI76_RS03535 0.80 -0.9 735,176 + BWI76_RS03535 0.80 +1.8 735,176 + BWI76_RS03535 0.80 -0.0 735,183 - BWI76_RS03535 0.80 +0.4 735,232 - BWI76_RS03535 0.84 -2.8 735,238 + BWI76_RS03535 0.84 +0.3 735,266 - BWI76_RS03535 0.86 -1.1 735,284 - BWI76_RS03535 0.88 +1.2 735,294 - BWI76_RS03535 0.88 +1.5 735,299 - BWI76_RS03535 0.89 +0.4 735,299 - BWI76_RS03535 0.89 +1.2 735,315 + +1.5 735,320 + -0.3 735,320 + +2.1 735,327 - -1.9 735,362 + +0.9 735,362 + +1.3 735,366 + +1.3 735,374 + +1.0 735,375 - -0.3 735,387 + -2.9 735,387 + -0.5 735,387 + -1.8 735,387 + +0.3 735,387 + +0.6 735,387 + +0.1 735,387 + -0.2 735,387 + -3.2 735,387 + +0.7 735,387 + -1.6 735,387 + -1.5 735,387 + +0.7 735,387 + +0.9 735,387 + -0.9 735,387 + +0.3 735,387 + +0.8 735,387 + +0.6 735,387 + +0.5 735,387 + -1.6 735,395 - -3.3 735,395 - -0.7 735,395 - -1.9 735,395 - +1.1 735,395 - +1.4 735,395 - -0.5 735,395 - -2.1 735,395 - +0.5 735,395 - -0.1 735,409 + -0.1 735,433 + +0.5 735,435 + +0.2 735,435 + +2.1 735,435 + +0.3 735,435 + -2.3 735,435 + +1.0 735,435 + +1.2 735,443 - -1.9 735,443 - -0.1 735,443 - -0.5 735,443 - -0.2 735,443 - -1.6 735,443 - +0.5 735,468 + +1.3 735,481 + +0.7 735,596 - BWI76_RS03540 0.20 -1.7 735,611 + BWI76_RS03540 0.35 -0.1 735,612 + BWI76_RS03540 0.36 -0.2 735,614 - BWI76_RS03540 0.39 -0.8 735,634 + BWI76_RS03540 0.59 -1.3 735,634 + BWI76_RS03540 0.59 -0.8 735,634 + BWI76_RS03540 0.59 -1.2 735,634 + BWI76_RS03540 0.59 +0.4 735,639 + BWI76_RS03540 0.65 -2.9 735,642 - BWI76_RS03540 0.68 -0.9 735,674 + -0.5 735,682 - -1.1 735,688 + -0.6 735,697 - -2.1 735,770 + -0.9 735,803 + +0.6 735,806 + +0.4 735,827 - -0.3 735,880 + -0.2 735,912 + +0.0 735,912 + +0.6 735,913 + +0.7 735,914 - -0.5 735,920 - -0.5 735,964 - BWI76_RS03545 0.15 +0.1 735,964 - BWI76_RS03545 0.15 -0.3 735,974 - BWI76_RS03545 0.18 +0.2 736,036 + BWI76_RS03545 0.37 +2.6 736,038 + BWI76_RS03545 0.38 -0.8 736,075 + BWI76_RS03545 0.49 -0.9 736,075 + BWI76_RS03545 0.49 -0.6 736,094 + BWI76_RS03545 0.55 +0.6 736,102 - BWI76_RS03545 0.57 +1.5 736,104 + BWI76_RS03545 0.58 -0.9 736,112 - BWI76_RS03545 0.60 -1.1 736,149 + BWI76_RS03545 0.71 -1.1 736,457 - BWI76_RS03550 0.47 -0.0 736,478 + BWI76_RS03550 0.52 +1.0 736,478 + BWI76_RS03550 0.52 -0.7 736,478 + BWI76_RS03550 0.52 +0.1 736,491 - BWI76_RS03550 0.55 -1.5 736,491 - BWI76_RS03550 0.55 -0.2 736,509 - BWI76_RS03550 0.59 +1.4 736,548 - BWI76_RS03550 0.68 -0.2 736,608 + BWI76_RS03550 0.83 +0.1
Or see this region's nucleotide sequence