Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03120 and BWI76_RS03125 are separated by 5 nucleotides BWI76_RS03125 and BWI76_RS03130 are separated by 10 nucleotides BWI76_RS03130 and BWI76_RS03135 overlap by 11 nucleotides
BWI76_RS03120: BWI76_RS03120 - ABC transporter ATP-binding protein, at 643,322 to 644,392
_RS03120
BWI76_RS03125: BWI76_RS03125 - phosphodiesterase, at 644,398 to 645,222
_RS03125
BWI76_RS03130: BWI76_RS03130 - putative SN-glycerol-3-phosphate transport system permease, at 645,233 to 646,120
_RS03130
BWI76_RS03135: BWI76_RS03135 - sugar ABC transporter permease, at 646,110 to 646,982
_RS03135
Position (kb) 645
646
647 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 644.247 kb on - strand, within BWI76_RS03120 at 644.269 kb on - strand, within BWI76_RS03120 at 644.616 kb on + strand at 644.699 kb on - strand, within BWI76_RS03125 at 644.908 kb on - strand, within BWI76_RS03125 at 644.965 kb on - strand, within BWI76_RS03125 at 645.084 kb on + strand, within BWI76_RS03125 at 645.103 kb on + strand, within BWI76_RS03125 at 645.103 kb on + strand, within BWI76_RS03125 at 645.105 kb on + strand, within BWI76_RS03125 at 645.462 kb on - strand, within BWI76_RS03130 at 645.476 kb on + strand, within BWI76_RS03130 at 645.484 kb on - strand, within BWI76_RS03130 at 645.484 kb on - strand, within BWI76_RS03130 at 645.523 kb on - strand, within BWI76_RS03130 at 645.634 kb on + strand, within BWI76_RS03130 at 645.801 kb on + strand, within BWI76_RS03130 at 645.984 kb on + strand, within BWI76_RS03130 at 646.016 kb on + strand, within BWI76_RS03130 at 646.024 kb on - strand, within BWI76_RS03130 at 646.254 kb on - strand, within BWI76_RS03135 at 646.254 kb on - strand, within BWI76_RS03135 at 646.321 kb on + strand, within BWI76_RS03135 at 646.421 kb on + strand, within BWI76_RS03135 at 646.524 kb on - strand, within BWI76_RS03135 at 646.541 kb on + strand, within BWI76_RS03135 at 646.598 kb on + strand, within BWI76_RS03135 at 646.606 kb on - strand, within BWI76_RS03135 at 646.606 kb on - strand, within BWI76_RS03135 at 646.606 kb on - strand, within BWI76_RS03135 at 646.639 kb on + strand, within BWI76_RS03135 at 646.647 kb on + strand, within BWI76_RS03135 at 646.768 kb on + strand, within BWI76_RS03135 at 646.768 kb on + strand, within BWI76_RS03135 at 646.881 kb on - strand, within BWI76_RS03135 at 646.887 kb on + strand, within BWI76_RS03135
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 644,247 - BWI76_RS03120 0.86 -1.0 644,269 - BWI76_RS03120 0.88 +1.3 644,616 + +1.3 644,699 - BWI76_RS03125 0.36 +1.1 644,908 - BWI76_RS03125 0.62 -0.2 644,965 - BWI76_RS03125 0.69 -0.0 645,084 + BWI76_RS03125 0.83 -0.4 645,103 + BWI76_RS03125 0.85 -1.3 645,103 + BWI76_RS03125 0.85 +0.1 645,105 + BWI76_RS03125 0.86 +0.9 645,462 - BWI76_RS03130 0.26 +0.3 645,476 + BWI76_RS03130 0.27 -0.9 645,484 - BWI76_RS03130 0.28 +0.4 645,484 - BWI76_RS03130 0.28 -0.2 645,523 - BWI76_RS03130 0.33 -0.3 645,634 + BWI76_RS03130 0.45 -0.3 645,801 + BWI76_RS03130 0.64 -1.5 645,984 + BWI76_RS03130 0.85 -1.1 646,016 + BWI76_RS03130 0.88 +0.7 646,024 - BWI76_RS03130 0.89 +0.6 646,254 - BWI76_RS03135 0.16 +1.4 646,254 - BWI76_RS03135 0.16 -0.2 646,321 + BWI76_RS03135 0.24 -0.2 646,421 + BWI76_RS03135 0.36 -3.1 646,524 - BWI76_RS03135 0.47 -0.3 646,541 + BWI76_RS03135 0.49 -1.1 646,598 + BWI76_RS03135 0.56 +0.5 646,606 - BWI76_RS03135 0.57 +0.4 646,606 - BWI76_RS03135 0.57 -0.2 646,606 - BWI76_RS03135 0.57 -0.3 646,639 + BWI76_RS03135 0.61 -1.2 646,647 + BWI76_RS03135 0.62 -0.7 646,768 + BWI76_RS03135 0.75 -1.6 646,768 + BWI76_RS03135 0.75 -4.0 646,881 - BWI76_RS03135 0.88 -0.4 646,887 + BWI76_RS03135 0.89 -0.8
Or see this region's nucleotide sequence