Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02170 and BWI76_RS02175 are separated by 364 nucleotides BWI76_RS02175 and BWI76_RS02180 are separated by 69 nucleotides BWI76_RS02180 and BWI76_RS02185 are separated by 33 nucleotides
BWI76_RS02170: BWI76_RS02170 - MurR/RpiR family transcriptional regulator, at 451,272 to 452,159
_RS02170
BWI76_RS02175: BWI76_RS02175 - ribose 5-phosphate isomerase B, at 452,524 to 452,973
_RS02175
BWI76_RS02180: BWI76_RS02180 - hypothetical protein, at 453,043 to 453,375
_RS02180
BWI76_RS02185: BWI76_RS02185 - phosphonate metabolism protein PhnP, at 453,409 to 454,167
_RS02185
Position (kb) 452
453 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 451.560 kb on + strand, within BWI76_RS02170 at 451.574 kb on - strand, within BWI76_RS02170 at 451.581 kb on + strand, within BWI76_RS02170 at 451.581 kb on + strand, within BWI76_RS02170 at 451.581 kb on + strand, within BWI76_RS02170 at 451.582 kb on + strand, within BWI76_RS02170 at 451.589 kb on - strand, within BWI76_RS02170 at 451.589 kb on - strand, within BWI76_RS02170 at 451.592 kb on - strand, within BWI76_RS02170 at 451.602 kb on + strand, within BWI76_RS02170 at 451.608 kb on - strand, within BWI76_RS02170 at 451.632 kb on - strand, within BWI76_RS02170 at 451.642 kb on + strand at 451.722 kb on + strand, within BWI76_RS02170 at 451.743 kb on - strand, within BWI76_RS02170 at 451.820 kb on - strand, within BWI76_RS02170 at 451.884 kb on + strand, within BWI76_RS02170 at 451.892 kb on - strand, within BWI76_RS02170 at 451.963 kb on - strand, within BWI76_RS02170 at 452.105 kb on + strand at 452.125 kb on - strand at 452.127 kb on + strand at 452.162 kb on - strand at 452.413 kb on + strand at 452.566 kb on - strand at 452.611 kb on - strand at 452.664 kb on - strand, within BWI76_RS02175 at 452.786 kb on - strand, within BWI76_RS02175 at 452.813 kb on - strand, within BWI76_RS02175 at 452.841 kb on - strand, within BWI76_RS02175 at 452.869 kb on - strand, within BWI76_RS02175 at 453.005 kb on + strand at 453.018 kb on - strand at 453.023 kb on + strand at 453.051 kb on + strand at 453.224 kb on - strand, within BWI76_RS02180 at 453.304 kb on + strand, within BWI76_RS02180 at 453.504 kb on + strand at 453.537 kb on + strand, within BWI76_RS02185 at 453.705 kb on - strand, within BWI76_RS02185 at 453.803 kb on - strand, within BWI76_RS02185 at 453.803 kb on - strand, within BWI76_RS02185 at 453.830 kb on + strand, within BWI76_RS02185 at 453.848 kb on - strand, within BWI76_RS02185 at 453.943 kb on + strand, within BWI76_RS02185
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 451,560 + BWI76_RS02170 0.32 +0.4 451,574 - BWI76_RS02170 0.34 +0.4 451,581 + BWI76_RS02170 0.35 -2.1 451,581 + BWI76_RS02170 0.35 -1.0 451,581 + BWI76_RS02170 0.35 -1.6 451,582 + BWI76_RS02170 0.35 -0.4 451,589 - BWI76_RS02170 0.36 -0.6 451,589 - BWI76_RS02170 0.36 +1.0 451,592 - BWI76_RS02170 0.36 -1.6 451,602 + BWI76_RS02170 0.37 +0.2 451,608 - BWI76_RS02170 0.38 -0.3 451,632 - BWI76_RS02170 0.41 +0.0 451,642 + +0.8 451,722 + BWI76_RS02170 0.51 -0.3 451,743 - BWI76_RS02170 0.53 -0.2 451,820 - BWI76_RS02170 0.62 +0.0 451,884 + BWI76_RS02170 0.69 -1.5 451,892 - BWI76_RS02170 0.70 -1.5 451,963 - BWI76_RS02170 0.78 -0.4 452,105 + +0.4 452,125 - +0.3 452,127 + +0.7 452,162 - -0.5 452,413 + -0.5 452,566 - +0.6 452,611 - -0.6 452,664 - BWI76_RS02175 0.31 +0.2 452,786 - BWI76_RS02175 0.58 +0.5 452,813 - BWI76_RS02175 0.64 +0.4 452,841 - BWI76_RS02175 0.70 +0.5 452,869 - BWI76_RS02175 0.77 -1.5 453,005 + -1.2 453,018 - +0.8 453,023 + -0.6 453,051 + -0.2 453,224 - BWI76_RS02180 0.54 +0.9 453,304 + BWI76_RS02180 0.78 -3.2 453,504 + +0.4 453,537 + BWI76_RS02185 0.17 -0.1 453,705 - BWI76_RS02185 0.39 -0.6 453,803 - BWI76_RS02185 0.52 -0.5 453,803 - BWI76_RS02185 0.52 +0.6 453,830 + BWI76_RS02185 0.55 +0.7 453,848 - BWI76_RS02185 0.58 -0.4 453,943 + BWI76_RS02185 0.70 -0.0
Or see this region's nucleotide sequence