Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01020 and BWI76_RS01025 are separated by 3 nucleotides BWI76_RS01025 and BWI76_RS01030 overlap by 17 nucleotides
BWI76_RS01020: BWI76_RS01020 - DNA helicase Rep, at 207,493 to 209,511
_RS01020
BWI76_RS01025: BWI76_RS01025 - hypothetical protein, at 209,515 to 210,477
_RS01025
BWI76_RS01030: BWI76_RS01030 - cytosine/purine, uracil, thiamine, allantoin permease family protein, at 210,461 to 211,876
_RS01030
Position (kb) 209
210
211 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 209.478 kb on + strand at 209.635 kb on + strand, within BWI76_RS01025 at 209.705 kb on + strand, within BWI76_RS01025 at 209.713 kb on - strand, within BWI76_RS01025 at 209.829 kb on - strand, within BWI76_RS01025 at 209.848 kb on + strand, within BWI76_RS01025 at 209.865 kb on - strand, within BWI76_RS01025 at 209.865 kb on - strand, within BWI76_RS01025 at 209.865 kb on - strand, within BWI76_RS01025 at 210.021 kb on + strand, within BWI76_RS01025 at 210.031 kb on + strand, within BWI76_RS01025 at 210.034 kb on - strand, within BWI76_RS01025 at 210.065 kb on + strand, within BWI76_RS01025 at 210.128 kb on - strand, within BWI76_RS01025 at 210.128 kb on - strand, within BWI76_RS01025 at 210.462 kb on - strand at 210.549 kb on + strand at 210.649 kb on + strand, within BWI76_RS01030 at 210.652 kb on - strand, within BWI76_RS01030 at 210.662 kb on - strand, within BWI76_RS01030 at 210.677 kb on + strand, within BWI76_RS01030 at 210.757 kb on + strand, within BWI76_RS01030 at 211.104 kb on + strand, within BWI76_RS01030 at 211.256 kb on - strand, within BWI76_RS01030 at 211.441 kb on + strand, within BWI76_RS01030
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 209,478 + -1.1 209,635 + BWI76_RS01025 0.12 -3.1 209,705 + BWI76_RS01025 0.20 -1.2 209,713 - BWI76_RS01025 0.21 -1.2 209,829 - BWI76_RS01025 0.33 +0.3 209,848 + BWI76_RS01025 0.35 -0.2 209,865 - BWI76_RS01025 0.36 -0.8 209,865 - BWI76_RS01025 0.36 +0.2 209,865 - BWI76_RS01025 0.36 +0.3 210,021 + BWI76_RS01025 0.53 -0.1 210,031 + BWI76_RS01025 0.54 -1.5 210,034 - BWI76_RS01025 0.54 -0.6 210,065 + BWI76_RS01025 0.57 -0.3 210,128 - BWI76_RS01025 0.64 -1.8 210,128 - BWI76_RS01025 0.64 -1.0 210,462 - +3.1 210,549 + +1.1 210,649 + BWI76_RS01030 0.13 -2.2 210,652 - BWI76_RS01030 0.13 -1.8 210,662 - BWI76_RS01030 0.14 -0.4 210,677 + BWI76_RS01030 0.15 -0.2 210,757 + BWI76_RS01030 0.21 -2.2 211,104 + BWI76_RS01030 0.45 -1.0 211,256 - BWI76_RS01030 0.56 -0.1 211,441 + BWI76_RS01030 0.69 +0.6
Or see this region's nucleotide sequence