Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00285 and BWI76_RS00290 are separated by 101 nucleotides BWI76_RS00290 and BWI76_RS00295 are separated by 12 nucleotides BWI76_RS00295 and BWI76_RS00300 overlap by 4 nucleotides
BWI76_RS00285: BWI76_RS00285 - D-ribose ABC transporter substrate-binding protein, at 59,243 to 60,133
_RS00285
BWI76_RS00290: BWI76_RS00290 - Ribokinase (EC 2.7.1.15) (from data) , at 60,235 to 61,164
_RS00290
BWI76_RS00295: BWI76_RS00295 - transcriptional regulator RbsR, at 61,177 to 62,160
_RS00295
BWI76_RS00300: BWI76_RS00300 - MFS transporter, at 62,157 to 63,554
_RS00300
Position (kb)
60
61
62 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 59.272 kb on - strand at 59.417 kb on - strand, within BWI76_RS00285 at 59.538 kb on - strand, within BWI76_RS00285 at 59.546 kb on - strand, within BWI76_RS00285 at 59.758 kb on + strand, within BWI76_RS00285 at 59.758 kb on + strand, within BWI76_RS00285 at 59.775 kb on + strand, within BWI76_RS00285 at 59.830 kb on - strand, within BWI76_RS00285 at 60.108 kb on + strand at 60.116 kb on - strand at 60.261 kb on - strand at 60.289 kb on + strand at 60.371 kb on + strand, within BWI76_RS00290 at 60.462 kb on + strand, within BWI76_RS00290 at 60.500 kb on - strand, within BWI76_RS00290 at 60.505 kb on - strand, within BWI76_RS00290 at 60.519 kb on - strand, within BWI76_RS00290 at 60.597 kb on - strand at 60.599 kb on - strand, within BWI76_RS00290 at 60.635 kb on + strand, within BWI76_RS00290 at 60.656 kb on + strand, within BWI76_RS00290 at 60.734 kb on - strand, within BWI76_RS00290 at 60.734 kb on - strand, within BWI76_RS00290 at 60.793 kb on + strand, within BWI76_RS00290 at 60.820 kb on - strand, within BWI76_RS00290 at 60.821 kb on - strand, within BWI76_RS00290 at 60.865 kb on - strand, within BWI76_RS00290 at 61.113 kb on - strand at 61.165 kb on + strand at 61.173 kb on - strand at 61.431 kb on + strand, within BWI76_RS00295 at 61.433 kb on + strand, within BWI76_RS00295 at 61.439 kb on + strand, within BWI76_RS00295 at 61.441 kb on - strand, within BWI76_RS00295 at 61.441 kb on - strand, within BWI76_RS00295 at 61.447 kb on - strand, within BWI76_RS00295 at 61.457 kb on - strand, within BWI76_RS00295 at 61.580 kb on - strand, within BWI76_RS00295 at 61.960 kb on + strand, within BWI76_RS00295 at 61.960 kb on + strand, within BWI76_RS00295 at 61.968 kb on - strand, within BWI76_RS00295 at 62.007 kb on + strand, within BWI76_RS00295 at 62.102 kb on + strand at 62.115 kb on - strand at 62.137 kb on - strand at 62.143 kb on - strand at 62.163 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 59,272 - +0.7 59,417 - BWI76_RS00285 0.20 +1.7 59,538 - BWI76_RS00285 0.33 +0.1 59,546 - BWI76_RS00285 0.34 -0.8 59,758 + BWI76_RS00285 0.58 +1.2 59,758 + BWI76_RS00285 0.58 +0.4 59,775 + BWI76_RS00285 0.60 +0.4 59,830 - BWI76_RS00285 0.66 +3.2 60,108 + +2.4 60,116 - +1.8 60,261 - +1.5 60,289 + +0.7 60,371 + BWI76_RS00290 0.15 +0.8 60,462 + BWI76_RS00290 0.24 +1.6 60,500 - BWI76_RS00290 0.28 +0.9 60,505 - BWI76_RS00290 0.29 +1.3 60,519 - BWI76_RS00290 0.31 +1.5 60,597 - +2.6 60,599 - BWI76_RS00290 0.39 +1.5 60,635 + BWI76_RS00290 0.43 +3.0 60,656 + BWI76_RS00290 0.45 +1.2 60,734 - BWI76_RS00290 0.54 +0.9 60,734 - BWI76_RS00290 0.54 +1.8 60,793 + BWI76_RS00290 0.60 +1.0 60,820 - BWI76_RS00290 0.63 +1.5 60,821 - BWI76_RS00290 0.63 +0.3 60,865 - BWI76_RS00290 0.68 +1.5 61,113 - +1.1 61,165 + +0.7 61,173 - +1.4 61,431 + BWI76_RS00295 0.26 +1.3 61,433 + BWI76_RS00295 0.26 +0.8 61,439 + BWI76_RS00295 0.27 +1.3 61,441 - BWI76_RS00295 0.27 -1.9 61,441 - BWI76_RS00295 0.27 +1.3 61,447 - BWI76_RS00295 0.27 +1.1 61,457 - BWI76_RS00295 0.28 +0.6 61,580 - BWI76_RS00295 0.41 -0.2 61,960 + BWI76_RS00295 0.80 +1.7 61,960 + BWI76_RS00295 0.80 +0.6 61,968 - BWI76_RS00295 0.80 +1.8 62,007 + BWI76_RS00295 0.84 +2.6 62,102 + +1.4 62,115 - +1.0 62,137 - +0.4 62,143 - +1.0 62,163 - +0.0
Or see this region's nucleotide sequence