Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27880 and BWI76_RS27885 overlap by 4 nucleotides BWI76_RS27885 and BWI76_RS27890 are separated by 341 nucleotides
BWI76_RS27880: BWI76_RS27880 - 6-phospho-alpha-glucosidase, at 5,781,523 to 5,782,845
_RS27880
BWI76_RS27885: BWI76_RS27885 - PTS alpha-glucoside transporter subunit IICB, at 5,782,842 to 5,784,464
_RS27885
BWI76_RS27890: BWI76_RS27890 - GntR family transcriptional regulator, at 5,784,806 to 5,785,552
_RS27890
Position (kb)
5782
5783
5784
5785 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5782.010 kb on + strand, within BWI76_RS27880 at 5782.044 kb on + strand, within BWI76_RS27880 at 5782.052 kb on - strand, within BWI76_RS27880 at 5782.053 kb on - strand, within BWI76_RS27880 at 5782.105 kb on - strand, within BWI76_RS27880 at 5782.191 kb on + strand, within BWI76_RS27880 at 5782.262 kb on - strand, within BWI76_RS27880 at 5782.295 kb on - strand, within BWI76_RS27880 at 5782.295 kb on - strand, within BWI76_RS27880 at 5782.404 kb on + strand, within BWI76_RS27880 at 5782.462 kb on + strand, within BWI76_RS27880 at 5782.643 kb on - strand, within BWI76_RS27880 at 5782.695 kb on + strand, within BWI76_RS27880 at 5782.716 kb on - strand at 5782.797 kb on + strand at 5782.797 kb on + strand at 5782.797 kb on + strand at 5782.805 kb on - strand at 5782.874 kb on - strand at 5782.900 kb on + strand at 5782.927 kb on - strand at 5782.967 kb on + strand at 5783.053 kb on + strand, within BWI76_RS27885 at 5783.053 kb on - strand, within BWI76_RS27885 at 5783.207 kb on + strand, within BWI76_RS27885 at 5783.215 kb on - strand, within BWI76_RS27885 at 5783.215 kb on - strand, within BWI76_RS27885 at 5783.393 kb on - strand, within BWI76_RS27885 at 5783.424 kb on - strand, within BWI76_RS27885 at 5783.515 kb on - strand, within BWI76_RS27885 at 5783.527 kb on - strand, within BWI76_RS27885 at 5783.527 kb on - strand, within BWI76_RS27885 at 5783.625 kb on - strand, within BWI76_RS27885 at 5783.628 kb on - strand, within BWI76_RS27885 at 5783.638 kb on - strand, within BWI76_RS27885 at 5783.745 kb on + strand, within BWI76_RS27885 at 5783.828 kb on + strand, within BWI76_RS27885 at 5783.855 kb on - strand, within BWI76_RS27885 at 5783.855 kb on - strand, within BWI76_RS27885 at 5783.876 kb on - strand, within BWI76_RS27885 at 5783.884 kb on - strand, within BWI76_RS27885 at 5783.942 kb on - strand, within BWI76_RS27885 at 5784.000 kb on + strand, within BWI76_RS27885 at 5784.141 kb on + strand, within BWI76_RS27885 at 5784.218 kb on - strand, within BWI76_RS27885 at 5784.224 kb on - strand, within BWI76_RS27885 at 5784.301 kb on - strand, within BWI76_RS27885 at 5784.327 kb on + strand at 5784.379 kb on - strand at 5784.429 kb on - strand at 5784.486 kb on + strand at 5784.501 kb on + strand at 5784.501 kb on + strand at 5784.501 kb on + strand at 5784.501 kb on + strand at 5784.509 kb on - strand at 5784.542 kb on + strand at 5784.683 kb on - strand at 5784.699 kb on + strand at 5784.699 kb on + strand at 5784.699 kb on + strand at 5784.699 kb on + strand at 5784.699 kb on + strand at 5784.707 kb on - strand at 5784.707 kb on - strand at 5784.707 kb on - strand at 5784.707 kb on - strand at 5784.790 kb on + strand at 5784.866 kb on - strand at 5784.957 kb on + strand, within BWI76_RS27890 at 5785.018 kb on - strand, within BWI76_RS27890 at 5785.066 kb on + strand, within BWI76_RS27890 at 5785.096 kb on - strand, within BWI76_RS27890 at 5785.301 kb on + strand, within BWI76_RS27890 at 5785.399 kb on + strand, within BWI76_RS27890 at 5785.446 kb on + strand, within BWI76_RS27890
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 5,782,010 + BWI76_RS27880 0.37 +0.6 5,782,044 + BWI76_RS27880 0.39 -1.1 5,782,052 - BWI76_RS27880 0.40 +0.1 5,782,053 - BWI76_RS27880 0.40 +1.6 5,782,105 - BWI76_RS27880 0.44 -0.1 5,782,191 + BWI76_RS27880 0.50 +0.7 5,782,262 - BWI76_RS27880 0.56 +2.2 5,782,295 - BWI76_RS27880 0.58 +0.7 5,782,295 - BWI76_RS27880 0.58 -0.7 5,782,404 + BWI76_RS27880 0.67 +0.2 5,782,462 + BWI76_RS27880 0.71 -0.3 5,782,643 - BWI76_RS27880 0.85 +0.0 5,782,695 + BWI76_RS27880 0.89 +1.1 5,782,716 - -0.8 5,782,797 + +1.1 5,782,797 + +0.9 5,782,797 + +0.6 5,782,805 - +1.0 5,782,874 - -0.3 5,782,900 + -0.2 5,782,927 - +0.7 5,782,967 + +1.5 5,783,053 + BWI76_RS27885 0.13 +3.3 5,783,053 - BWI76_RS27885 0.13 +0.3 5,783,207 + BWI76_RS27885 0.22 +1.0 5,783,215 - BWI76_RS27885 0.23 +0.0 5,783,215 - BWI76_RS27885 0.23 +1.0 5,783,393 - BWI76_RS27885 0.34 +0.9 5,783,424 - BWI76_RS27885 0.36 +0.1 5,783,515 - BWI76_RS27885 0.41 +0.3 5,783,527 - BWI76_RS27885 0.42 +0.9 5,783,527 - BWI76_RS27885 0.42 +0.3 5,783,625 - BWI76_RS27885 0.48 -1.1 5,783,628 - BWI76_RS27885 0.48 +0.5 5,783,638 - BWI76_RS27885 0.49 -0.2 5,783,745 + BWI76_RS27885 0.56 +1.5 5,783,828 + BWI76_RS27885 0.61 +1.9 5,783,855 - BWI76_RS27885 0.62 -1.2 5,783,855 - BWI76_RS27885 0.62 +0.3 5,783,876 - BWI76_RS27885 0.64 -0.6 5,783,884 - BWI76_RS27885 0.64 +0.2 5,783,942 - BWI76_RS27885 0.68 +0.0 5,784,000 + BWI76_RS27885 0.71 -0.0 5,784,141 + BWI76_RS27885 0.80 +0.1 5,784,218 - BWI76_RS27885 0.85 +0.7 5,784,224 - BWI76_RS27885 0.85 +0.5 5,784,301 - BWI76_RS27885 0.90 +0.1 5,784,327 + -2.5 5,784,379 - -0.9 5,784,429 - +1.6 5,784,486 + +0.2 5,784,501 + +2.4 5,784,501 + -0.9 5,784,501 + +1.1 5,784,501 + +0.9 5,784,509 - +0.3 5,784,542 + +0.2 5,784,683 - -0.5 5,784,699 + +1.1 5,784,699 + +0.0 5,784,699 + +0.7 5,784,699 + +0.8 5,784,699 + +0.2 5,784,707 - +0.4 5,784,707 - +0.5 5,784,707 - +0.2 5,784,707 - -1.0 5,784,790 + -1.4 5,784,866 - +0.1 5,784,957 + BWI76_RS27890 0.20 -1.3 5,785,018 - BWI76_RS27890 0.28 +0.4 5,785,066 + BWI76_RS27890 0.35 +1.4 5,785,096 - BWI76_RS27890 0.39 +0.6 5,785,301 + BWI76_RS27890 0.66 +0.1 5,785,399 + BWI76_RS27890 0.79 -0.0 5,785,446 + BWI76_RS27890 0.86 +0.6
Or see this region's nucleotide sequence