Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27520 and BWI76_RS27525 are separated by 287 nucleotides BWI76_RS27525 and BWI76_RS27530 are separated by 145 nucleotides
BWI76_RS27520: BWI76_RS27520 - sodium/glutamate symporter, at 5,709,926 to 5,711,128
_RS27520
BWI76_RS27525: BWI76_RS27525 - xanthine permease XanP, at 5,711,416 to 5,712,807
_RS27525
BWI76_RS27530: BWI76_RS27530 - AsmA family protein, at 5,712,953 to 5,714,668
_RS27530
Position (kb) 5711
5712
5713 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5710.443 kb on - strand, within BWI76_RS27520 at 5710.891 kb on + strand, within BWI76_RS27520 at 5711.140 kb on + strand at 5711.399 kb on + strand at 5711.513 kb on + strand at 5711.541 kb on - strand at 5711.593 kb on + strand, within BWI76_RS27525 at 5711.601 kb on - strand, within BWI76_RS27525 at 5711.601 kb on - strand, within BWI76_RS27525 at 5711.601 kb on - strand, within BWI76_RS27525 at 5711.696 kb on + strand, within BWI76_RS27525 at 5711.706 kb on + strand, within BWI76_RS27525 at 5711.734 kb on - strand, within BWI76_RS27525 at 5711.756 kb on - strand, within BWI76_RS27525 at 5711.780 kb on + strand, within BWI76_RS27525 at 5711.819 kb on - strand, within BWI76_RS27525 at 5711.837 kb on + strand, within BWI76_RS27525 at 5711.842 kb on - strand, within BWI76_RS27525 at 5711.842 kb on - strand, within BWI76_RS27525 at 5711.845 kb on - strand, within BWI76_RS27525 at 5711.848 kb on + strand, within BWI76_RS27525 at 5711.888 kb on - strand, within BWI76_RS27525 at 5711.908 kb on + strand, within BWI76_RS27525 at 5711.952 kb on - strand, within BWI76_RS27525 at 5711.990 kb on + strand, within BWI76_RS27525 at 5712.073 kb on - strand, within BWI76_RS27525 at 5712.195 kb on + strand, within BWI76_RS27525 at 5712.197 kb on + strand, within BWI76_RS27525 at 5712.197 kb on + strand, within BWI76_RS27525 at 5712.203 kb on - strand, within BWI76_RS27525 at 5712.228 kb on - strand, within BWI76_RS27525 at 5712.390 kb on + strand, within BWI76_RS27525 at 5712.398 kb on - strand, within BWI76_RS27525 at 5712.398 kb on - strand, within BWI76_RS27525 at 5712.408 kb on + strand, within BWI76_RS27525 at 5712.424 kb on + strand, within BWI76_RS27525 at 5712.482 kb on + strand, within BWI76_RS27525 at 5712.578 kb on - strand, within BWI76_RS27525 at 5712.578 kb on - strand, within BWI76_RS27525 at 5712.630 kb on - strand, within BWI76_RS27525 at 5712.713 kb on - strand at 5712.811 kb on + strand at 5712.884 kb on + strand at 5712.891 kb on + strand at 5713.037 kb on - strand at 5713.298 kb on - strand, within BWI76_RS27530 at 5713.306 kb on - strand, within BWI76_RS27530 at 5713.328 kb on - strand, within BWI76_RS27530 at 5713.362 kb on + strand, within BWI76_RS27530 at 5713.370 kb on - strand, within BWI76_RS27530 at 5713.546 kb on - strand, within BWI76_RS27530 at 5713.586 kb on + strand, within BWI76_RS27530 at 5713.716 kb on + strand, within BWI76_RS27530 at 5713.724 kb on - strand, within BWI76_RS27530 at 5713.744 kb on - strand, within BWI76_RS27530 at 5713.744 kb on - strand, within BWI76_RS27530 at 5713.781 kb on - strand, within BWI76_RS27530 at 5713.781 kb on - strand, within BWI76_RS27530 at 5713.781 kb on - strand, within BWI76_RS27530 at 5713.781 kb on - strand, within BWI76_RS27530 at 5713.781 kb on - strand, within BWI76_RS27530 at 5713.788 kb on + strand, within BWI76_RS27530 at 5713.801 kb on - strand, within BWI76_RS27530
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 5,710,443 - BWI76_RS27520 0.43 +1.5 5,710,891 + BWI76_RS27520 0.80 -1.2 5,711,140 + -0.1 5,711,399 + +0.7 5,711,513 + -0.1 5,711,541 - -1.4 5,711,593 + BWI76_RS27525 0.13 -1.8 5,711,601 - BWI76_RS27525 0.13 -0.2 5,711,601 - BWI76_RS27525 0.13 +0.6 5,711,601 - BWI76_RS27525 0.13 +0.8 5,711,696 + BWI76_RS27525 0.20 -3.0 5,711,706 + BWI76_RS27525 0.21 -2.6 5,711,734 - BWI76_RS27525 0.23 -0.7 5,711,756 - BWI76_RS27525 0.24 -0.1 5,711,780 + BWI76_RS27525 0.26 +0.6 5,711,819 - BWI76_RS27525 0.29 +2.1 5,711,837 + BWI76_RS27525 0.30 -2.0 5,711,842 - BWI76_RS27525 0.31 +0.6 5,711,842 - BWI76_RS27525 0.31 +1.7 5,711,845 - BWI76_RS27525 0.31 -1.5 5,711,848 + BWI76_RS27525 0.31 -2.1 5,711,888 - BWI76_RS27525 0.34 -0.3 5,711,908 + BWI76_RS27525 0.35 -0.9 5,711,952 - BWI76_RS27525 0.39 +1.6 5,711,990 + BWI76_RS27525 0.41 +1.7 5,712,073 - BWI76_RS27525 0.47 -2.0 5,712,195 + BWI76_RS27525 0.56 -0.2 5,712,197 + BWI76_RS27525 0.56 +1.5 5,712,197 + BWI76_RS27525 0.56 -0.0 5,712,203 - BWI76_RS27525 0.57 -0.4 5,712,228 - BWI76_RS27525 0.58 +0.2 5,712,390 + BWI76_RS27525 0.70 -0.1 5,712,398 - BWI76_RS27525 0.71 -0.3 5,712,398 - BWI76_RS27525 0.71 -0.1 5,712,408 + BWI76_RS27525 0.71 +0.1 5,712,424 + BWI76_RS27525 0.72 -1.0 5,712,482 + BWI76_RS27525 0.77 -1.8 5,712,578 - BWI76_RS27525 0.83 +0.1 5,712,578 - BWI76_RS27525 0.83 -0.0 5,712,630 - BWI76_RS27525 0.87 -0.9 5,712,713 - -0.0 5,712,811 + +0.5 5,712,884 + -0.5 5,712,891 + +0.6 5,713,037 - +0.1 5,713,298 - BWI76_RS27530 0.20 -0.2 5,713,306 - BWI76_RS27530 0.21 +0.4 5,713,328 - BWI76_RS27530 0.22 -0.1 5,713,362 + BWI76_RS27530 0.24 -0.3 5,713,370 - BWI76_RS27530 0.24 -1.1 5,713,546 - BWI76_RS27530 0.35 -0.0 5,713,586 + BWI76_RS27530 0.37 -1.1 5,713,716 + BWI76_RS27530 0.44 +0.5 5,713,724 - BWI76_RS27530 0.45 -0.9 5,713,744 - BWI76_RS27530 0.46 +1.3 5,713,744 - BWI76_RS27530 0.46 +0.3 5,713,781 - BWI76_RS27530 0.48 +1.0 5,713,781 - BWI76_RS27530 0.48 +1.4 5,713,781 - BWI76_RS27530 0.48 -0.9 5,713,781 - BWI76_RS27530 0.48 -0.1 5,713,781 - BWI76_RS27530 0.48 +0.5 5,713,788 + BWI76_RS27530 0.49 +1.0 5,713,801 - BWI76_RS27530 0.49 -1.5
Or see this region's nucleotide sequence