Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27400 and BWI76_RS27405 are separated by 275 nucleotides BWI76_RS27405 and BWI76_RS27410 are separated by 5 nucleotides
BWI76_RS27400: BWI76_RS27400 - chloride channel protein, at 5,684,883 to 5,686,142
_RS27400
BWI76_RS27405: BWI76_RS27405 - membrane protein, at 5,686,418 to 5,687,035
_RS27405
BWI76_RS27410: BWI76_RS27410 - DNA ligase B, at 5,687,041 to 5,688,717
_RS27410
Position (kb)
5686
5687
5688 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5685.446 kb on + strand, within BWI76_RS27400 at 5685.587 kb on - strand, within BWI76_RS27400 at 5685.619 kb on + strand, within BWI76_RS27400 at 5685.683 kb on + strand, within BWI76_RS27400 at 5685.773 kb on - strand, within BWI76_RS27400 at 5685.784 kb on - strand, within BWI76_RS27400 at 5685.797 kb on - strand, within BWI76_RS27400 at 5685.843 kb on - strand, within BWI76_RS27400 at 5685.890 kb on + strand, within BWI76_RS27400 at 5685.954 kb on - strand, within BWI76_RS27400 at 5685.967 kb on - strand, within BWI76_RS27400 at 5685.998 kb on + strand, within BWI76_RS27400 at 5686.006 kb on - strand, within BWI76_RS27400 at 5686.152 kb on + strand at 5686.224 kb on + strand at 5686.224 kb on + strand at 5686.231 kb on - strand at 5686.231 kb on - strand at 5686.232 kb on - strand at 5686.421 kb on - strand at 5686.421 kb on - strand at 5686.460 kb on + strand at 5686.545 kb on - strand, within BWI76_RS27405 at 5686.590 kb on + strand, within BWI76_RS27405 at 5686.592 kb on + strand, within BWI76_RS27405 at 5686.598 kb on - strand, within BWI76_RS27405 at 5686.634 kb on + strand, within BWI76_RS27405 at 5686.784 kb on + strand, within BWI76_RS27405 at 5686.816 kb on - strand, within BWI76_RS27405 at 5686.846 kb on - strand, within BWI76_RS27405 at 5686.864 kb on - strand, within BWI76_RS27405 at 5686.899 kb on - strand, within BWI76_RS27405 at 5686.899 kb on - strand at 5686.986 kb on + strand at 5687.283 kb on + strand, within BWI76_RS27410 at 5687.316 kb on + strand, within BWI76_RS27410 at 5687.413 kb on - strand, within BWI76_RS27410 at 5687.515 kb on + strand, within BWI76_RS27410 at 5687.515 kb on + strand, within BWI76_RS27410 at 5687.557 kb on + strand, within BWI76_RS27410 at 5687.557 kb on + strand, within BWI76_RS27410 at 5687.580 kb on - strand, within BWI76_RS27410 at 5687.757 kb on + strand, within BWI76_RS27410 at 5687.786 kb on - strand, within BWI76_RS27410 at 5687.941 kb on - strand, within BWI76_RS27410 at 5688.026 kb on - strand, within BWI76_RS27410
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 5,685,446 + BWI76_RS27400 0.45 -1.1 5,685,587 - BWI76_RS27400 0.56 +0.1 5,685,619 + BWI76_RS27400 0.58 -0.7 5,685,683 + BWI76_RS27400 0.63 +0.7 5,685,773 - BWI76_RS27400 0.71 -0.3 5,685,784 - BWI76_RS27400 0.72 +0.2 5,685,797 - BWI76_RS27400 0.73 -1.6 5,685,843 - BWI76_RS27400 0.76 -0.0 5,685,890 + BWI76_RS27400 0.80 -0.4 5,685,954 - BWI76_RS27400 0.85 +1.3 5,685,967 - BWI76_RS27400 0.86 +1.0 5,685,998 + BWI76_RS27400 0.88 -1.6 5,686,006 - BWI76_RS27400 0.89 -2.7 5,686,152 + -1.1 5,686,224 + +0.2 5,686,224 + -2.1 5,686,231 - -0.2 5,686,231 - -0.1 5,686,232 - +0.1 5,686,421 - +0.6 5,686,421 - -0.2 5,686,460 + +3.7 5,686,545 - BWI76_RS27405 0.21 +0.4 5,686,590 + BWI76_RS27405 0.28 +0.1 5,686,592 + BWI76_RS27405 0.28 -0.1 5,686,598 - BWI76_RS27405 0.29 +0.8 5,686,634 + BWI76_RS27405 0.35 +3.6 5,686,784 + BWI76_RS27405 0.59 +1.1 5,686,816 - BWI76_RS27405 0.64 +0.7 5,686,846 - BWI76_RS27405 0.69 +0.1 5,686,864 - BWI76_RS27405 0.72 +0.9 5,686,899 - BWI76_RS27405 0.78 -0.7 5,686,899 - -0.0 5,686,986 + +0.1 5,687,283 + BWI76_RS27410 0.14 -0.5 5,687,316 + BWI76_RS27410 0.16 +0.7 5,687,413 - BWI76_RS27410 0.22 +1.0 5,687,515 + BWI76_RS27410 0.28 +0.5 5,687,515 + BWI76_RS27410 0.28 +0.2 5,687,557 + BWI76_RS27410 0.31 -0.0 5,687,557 + BWI76_RS27410 0.31 +0.1 5,687,580 - BWI76_RS27410 0.32 -0.1 5,687,757 + BWI76_RS27410 0.43 -0.2 5,687,786 - BWI76_RS27410 0.44 +1.3 5,687,941 - BWI76_RS27410 0.54 +0.5 5,688,026 - BWI76_RS27410 0.59 +0.3
Or see this region's nucleotide sequence