Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21275 and BWI76_RS21280 overlap by 4 nucleotides BWI76_RS21280 and BWI76_RS21285 are separated by 19 nucleotides BWI76_RS21285 and BWI76_RS21290 are separated by 209 nucleotides
BWI76_RS21275: BWI76_RS21275 - lytic transglycosylase F, at 4,456,991 to 4,458,607
_RS21275
BWI76_RS21280: BWI76_RS21280 - tRNA adenosine(34) deaminase TadA, at 4,458,604 to 4,459,149
_RS21280
BWI76_RS21285: BWI76_RS21285 - HAD family hydrolase, at 4,459,169 to 4,459,804
_RS21285
BWI76_RS21290: BWI76_RS21290 - DNA-binding transcriptional regulator, at 4,460,014 to 4,460,862
_RS21290
Position (kb) 4459
4460 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4458.374 kb on + strand, within BWI76_RS21275 at 4458.380 kb on + strand, within BWI76_RS21275 at 4458.382 kb on - strand, within BWI76_RS21275 at 4458.492 kb on - strand at 4458.607 kb on + strand at 4459.171 kb on + strand at 4459.398 kb on + strand, within BWI76_RS21285 at 4459.745 kb on - strand at 4459.988 kb on + strand at 4459.996 kb on - strand at 4460.011 kb on + strand at 4460.045 kb on + strand at 4460.053 kb on - strand at 4460.053 kb on - strand at 4460.256 kb on + strand, within BWI76_RS21290 at 4460.256 kb on + strand, within BWI76_RS21290 at 4460.333 kb on - strand, within BWI76_RS21290 at 4460.531 kb on + strand, within BWI76_RS21290 at 4460.579 kb on - strand, within BWI76_RS21290 at 4460.748 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 4,458,374 + BWI76_RS21275 0.86 -3.0 4,458,380 + BWI76_RS21275 0.86 +1.1 4,458,382 - BWI76_RS21275 0.86 -0.7 4,458,492 - +0.4 4,458,607 + -0.2 4,459,171 + -0.1 4,459,398 + BWI76_RS21285 0.36 +1.1 4,459,745 - -1.4 4,459,988 + +0.7 4,459,996 - +0.0 4,460,011 + +0.6 4,460,045 + +0.4 4,460,053 - -0.3 4,460,053 - +0.8 4,460,256 + BWI76_RS21290 0.29 -2.2 4,460,256 + BWI76_RS21290 0.29 +0.4 4,460,333 - BWI76_RS21290 0.38 +1.5 4,460,531 + BWI76_RS21290 0.61 -0.1 4,460,579 - BWI76_RS21290 0.67 -0.0 4,460,748 - +0.5
Or see this region's nucleotide sequence