Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20235 and BWI76_RS20240 are separated by 76 nucleotides BWI76_RS20240 and BWI76_RS20245 are separated by 46 nucleotides BWI76_RS20245 and BWI76_RS20250 overlap by 8 nucleotides
BWI76_RS20235: BWI76_RS20235 - acetate kinase, at 4,240,377 to 4,241,579
_RS20235
BWI76_RS20240: BWI76_RS20240 - phosphate acetyltransferase, at 4,241,656 to 4,243,800
_RS20240
BWI76_RS20245: BWI76_RS20245 - transketolase subunit B, at 4,243,847 to 4,244,800
_RS20245
BWI76_RS20250: BWI76_RS20250 - hypothetical protein, at 4,244,793 to 4,245,623
_RS20250
Position (kb) 4241
4242
4243
4244 Strain fitness (log2 ratio) -1
0
1
2 at 4240.747 kb on + strand, within BWI76_RS20235 at 4241.607 kb on + strand at 4241.964 kb on - strand, within BWI76_RS20240 at 4242.998 kb on + strand, within BWI76_RS20240 at 4243.710 kb on + strand at 4243.750 kb on + strand at 4243.928 kb on - strand at 4243.942 kb on + strand at 4243.976 kb on + strand, within BWI76_RS20245 at 4244.137 kb on - strand, within BWI76_RS20245 at 4244.455 kb on + strand, within BWI76_RS20245 at 4244.507 kb on - strand, within BWI76_RS20245 at 4244.668 kb on + strand, within BWI76_RS20245 at 4244.742 kb on + strand at 4244.791 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 4,240,747 + BWI76_RS20235 0.31 +0.1 4,241,607 + -1.2 4,241,964 - BWI76_RS20240 0.14 -1.2 4,242,998 + BWI76_RS20240 0.63 -1.4 4,243,710 + -1.1 4,243,750 + -0.4 4,243,928 - -0.3 4,243,942 + -0.1 4,243,976 + BWI76_RS20245 0.14 -0.9 4,244,137 - BWI76_RS20245 0.30 +0.0 4,244,455 + BWI76_RS20245 0.64 +2.0 4,244,507 - BWI76_RS20245 0.69 +0.1 4,244,668 + BWI76_RS20245 0.86 +1.3 4,244,742 + -0.8 4,244,791 + +0.4
Or see this region's nucleotide sequence