Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19480 and BWI76_RS19485 are separated by 159 nucleotides BWI76_RS19485 and BWI76_RS19490 overlap by 26 nucleotides BWI76_RS19490 and BWI76_RS19495 overlap by 17 nucleotides BWI76_RS19495 and BWI76_RS19500 overlap by 8 nucleotides
BWI76_RS19480: BWI76_RS19480 - transcriptional regulator, at 4,071,356 to 4,072,114
_RS19480
BWI76_RS19485: BWI76_RS19485 - membrane protein, at 4,072,274 to 4,072,387
_RS19485
BWI76_RS19490: BWI76_RS19490 - binding-protein-dependent transport system inner membrane protein, at 4,072,362 to 4,073,099
_RS19490
BWI76_RS19495: BWI76_RS19495 - ABC transporter, quaternary amine uptake transporter (QAT) family, ATP-binding protein, at 4,073,083 to 4,074,030
_RS19495
BWI76_RS19500: BWI76_RS19500 - osmoprotectant uptake system permease, at 4,074,023 to 4,075,180
_RS19500
Position (kb)
4072
4073
4074 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4071.663 kb on + strand, within BWI76_RS19480 at 4071.682 kb on + strand, within BWI76_RS19480 at 4071.906 kb on - strand, within BWI76_RS19480 at 4072.173 kb on - strand at 4072.179 kb on + strand at 4072.249 kb on - strand at 4072.623 kb on - strand, within BWI76_RS19490 at 4072.733 kb on + strand, within BWI76_RS19490 at 4072.941 kb on - strand at 4073.016 kb on - strand, within BWI76_RS19490 at 4073.756 kb on + strand, within BWI76_RS19495 at 4074.047 kb on - strand at 4074.077 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 4,071,663 + BWI76_RS19480 0.40 -2.3 4,071,682 + BWI76_RS19480 0.43 -1.6 4,071,906 - BWI76_RS19480 0.72 +0.2 4,072,173 - +0.2 4,072,179 + -0.1 4,072,249 - -0.2 4,072,623 - BWI76_RS19490 0.35 -2.6 4,072,733 + BWI76_RS19490 0.50 -1.1 4,072,941 - +1.9 4,073,016 - BWI76_RS19490 0.89 -0.3 4,073,756 + BWI76_RS19495 0.71 -0.6 4,074,047 - -0.3 4,074,077 + +1.5
Or see this region's nucleotide sequence