Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19450 and BWI76_RS19455 overlap by 4 nucleotides BWI76_RS19455 and BWI76_RS19460 are separated by 12 nucleotides BWI76_RS19460 and BWI76_RS19465 are separated by 110 nucleotides
BWI76_RS19450: BWI76_RS19450 - hypothetical protein, at 4,064,499 to 4,065,635
_RS19450
BWI76_RS19455: BWI76_RS19455 - hypothetical protein, at 4,065,632 to 4,067,638
_RS19455
BWI76_RS19460: BWI76_RS19460 - lipoprotein, at 4,067,651 to 4,068,112
_RS19460
BWI76_RS19465: BWI76_RS19465 - hypothetical protein, at 4,068,223 to 4,068,690
_RS19465
Position (kb)
4065
4066
4067
4068 Strain fitness (log2 ratio)
-2
-1
0
1 at 4064.653 kb on + strand, within BWI76_RS19450 at 4064.725 kb on + strand, within BWI76_RS19450 at 4064.867 kb on - strand, within BWI76_RS19450 at 4065.057 kb on + strand, within BWI76_RS19450 at 4065.162 kb on + strand, within BWI76_RS19450 at 4065.696 kb on - strand at 4065.765 kb on - strand at 4065.797 kb on + strand at 4066.192 kb on + strand, within BWI76_RS19455 at 4066.619 kb on + strand, within BWI76_RS19455 at 4066.931 kb on - strand, within BWI76_RS19455 at 4067.141 kb on + strand, within BWI76_RS19455 at 4067.168 kb on + strand, within BWI76_RS19455 at 4067.226 kb on - strand, within BWI76_RS19455 at 4067.320 kb on + strand, within BWI76_RS19455 at 4067.431 kb on + strand, within BWI76_RS19455 at 4067.613 kb on - strand at 4067.766 kb on - strand, within BWI76_RS19460 at 4067.785 kb on - strand, within BWI76_RS19460 at 4067.824 kb on + strand, within BWI76_RS19460 at 4067.911 kb on - strand, within BWI76_RS19460 at 4068.120 kb on - strand at 4068.441 kb on - strand, within BWI76_RS19465 at 4068.528 kb on + strand, within BWI76_RS19465 at 4068.530 kb on - strand, within BWI76_RS19465
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 4,064,653 + BWI76_RS19450 0.14 -0.1 4,064,725 + BWI76_RS19450 0.20 -0.8 4,064,867 - BWI76_RS19450 0.32 -0.2 4,065,057 + BWI76_RS19450 0.49 -0.3 4,065,162 + BWI76_RS19450 0.58 +0.1 4,065,696 - +1.1 4,065,765 - -0.4 4,065,797 + -0.4 4,066,192 + BWI76_RS19455 0.28 -0.5 4,066,619 + BWI76_RS19455 0.49 -1.6 4,066,931 - BWI76_RS19455 0.65 -0.1 4,067,141 + BWI76_RS19455 0.75 -0.6 4,067,168 + BWI76_RS19455 0.77 -1.8 4,067,226 - BWI76_RS19455 0.79 -0.7 4,067,320 + BWI76_RS19455 0.84 +0.9 4,067,431 + BWI76_RS19455 0.90 -1.6 4,067,613 - +0.7 4,067,766 - BWI76_RS19460 0.25 -1.7 4,067,785 - BWI76_RS19460 0.29 +0.4 4,067,824 + BWI76_RS19460 0.37 -2.1 4,067,911 - BWI76_RS19460 0.56 -0.8 4,068,120 - -1.9 4,068,441 - BWI76_RS19465 0.47 -1.1 4,068,528 + BWI76_RS19465 0.65 +0.2 4,068,530 - BWI76_RS19465 0.66 -0.7
Or see this region's nucleotide sequence