Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17550 and BWI76_RS17555 are separated by 510 nucleotides BWI76_RS17555 and BWI76_RS17560 are separated by 136 nucleotides
BWI76_RS17550: BWI76_RS17550 - oxidoreductase (Fe-S)-binding subunit, at 3,641,215 to 3,643,155
_RS17550
BWI76_RS17555: BWI76_RS17555 - permease, at 3,643,666 to 3,645,138
_RS17555
BWI76_RS17560: BWI76_RS17560 - formate dehydrogenase subunit alpha, at 3,645,275 to 3,647,422
_RS17560
Position (kb)
3643
3644
3645
3646 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3642.698 kb on + strand, within BWI76_RS17550 at 3642.774 kb on - strand, within BWI76_RS17550 at 3642.922 kb on + strand, within BWI76_RS17550 at 3642.992 kb on + strand at 3643.077 kb on - strand at 3643.096 kb on - strand at 3643.098 kb on + strand at 3643.098 kb on + strand at 3643.098 kb on + strand at 3643.098 kb on + strand at 3643.098 kb on + strand at 3643.098 kb on + strand at 3643.101 kb on - strand at 3643.106 kb on - strand at 3643.106 kb on - strand at 3643.106 kb on - strand at 3643.106 kb on - strand at 3643.113 kb on + strand at 3643.113 kb on + strand at 3643.171 kb on + strand at 3643.180 kb on - strand at 3643.180 kb on - strand at 3643.180 kb on - strand at 3643.192 kb on - strand at 3643.243 kb on - strand at 3643.334 kb on + strand at 3643.334 kb on + strand at 3643.342 kb on - strand at 3643.342 kb on - strand at 3643.342 kb on - strand at 3643.342 kb on - strand at 3643.397 kb on + strand at 3643.397 kb on - strand at 3643.531 kb on + strand at 3643.531 kb on + strand at 3643.531 kb on + strand at 3643.531 kb on + strand at 3643.538 kb on - strand at 3643.539 kb on - strand at 3643.539 kb on - strand at 3643.539 kb on - strand at 3643.543 kb on + strand at 3643.543 kb on + strand at 3643.543 kb on + strand at 3643.543 kb on + strand at 3643.543 kb on + strand at 3643.543 kb on + strand at 3643.543 kb on + strand at 3643.543 kb on + strand at 3643.543 kb on + strand at 3643.543 kb on + strand at 3643.549 kb on - strand at 3643.551 kb on - strand at 3643.551 kb on - strand at 3643.553 kb on + strand at 3643.561 kb on - strand at 3643.567 kb on + strand at 3643.573 kb on - strand at 3643.591 kb on - strand at 3643.621 kb on + strand at 3643.626 kb on + strand at 3643.626 kb on + strand at 3643.626 kb on + strand at 3643.626 kb on + strand at 3643.626 kb on + strand at 3643.634 kb on - strand at 3643.636 kb on - strand at 3643.636 kb on - strand at 3643.645 kb on - strand at 3643.646 kb on + strand at 3643.659 kb on - strand at 3643.848 kb on - strand, within BWI76_RS17555 at 3643.854 kb on + strand, within BWI76_RS17555 at 3643.933 kb on - strand, within BWI76_RS17555 at 3643.942 kb on + strand, within BWI76_RS17555 at 3643.968 kb on + strand at 3644.023 kb on + strand, within BWI76_RS17555 at 3644.084 kb on - strand, within BWI76_RS17555 at 3644.314 kb on + strand, within BWI76_RS17555 at 3644.314 kb on + strand, within BWI76_RS17555 at 3644.324 kb on + strand, within BWI76_RS17555 at 3644.332 kb on - strand, within BWI76_RS17555 at 3644.332 kb on - strand, within BWI76_RS17555 at 3644.339 kb on + strand, within BWI76_RS17555 at 3644.659 kb on + strand, within BWI76_RS17555 at 3644.792 kb on + strand, within BWI76_RS17555 at 3644.826 kb on + strand, within BWI76_RS17555 at 3644.852 kb on + strand, within BWI76_RS17555 at 3645.060 kb on + strand at 3645.132 kb on - strand at 3645.251 kb on + strand at 3645.659 kb on - strand, within BWI76_RS17560 at 3645.950 kb on - strand, within BWI76_RS17560
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 3,642,698 + BWI76_RS17550 0.76 -0.2 3,642,774 - BWI76_RS17550 0.80 -0.1 3,642,922 + BWI76_RS17550 0.88 -0.3 3,642,992 + -0.2 3,643,077 - +0.6 3,643,096 - +0.6 3,643,098 + -1.6 3,643,098 + -1.0 3,643,098 + -0.0 3,643,098 + +1.9 3,643,098 + +0.6 3,643,098 + -1.4 3,643,101 - +0.2 3,643,106 - -0.4 3,643,106 - +1.2 3,643,106 - -0.8 3,643,106 - +0.6 3,643,113 + -1.1 3,643,113 + -0.4 3,643,171 + -1.9 3,643,180 - -0.4 3,643,180 - -1.3 3,643,180 - -1.0 3,643,192 - +0.4 3,643,243 - +0.7 3,643,334 + +0.3 3,643,334 + +0.4 3,643,342 - +1.0 3,643,342 - -0.2 3,643,342 - +0.6 3,643,342 - +0.8 3,643,397 + +0.8 3,643,397 - -0.4 3,643,531 + -2.0 3,643,531 + -2.2 3,643,531 + +2.2 3,643,531 + +0.2 3,643,538 - -0.2 3,643,539 - -0.3 3,643,539 - -0.0 3,643,539 - +1.0 3,643,543 + -1.0 3,643,543 + +0.8 3,643,543 + -0.1 3,643,543 + +1.1 3,643,543 + -1.1 3,643,543 + -0.5 3,643,543 + -1.4 3,643,543 + -0.7 3,643,543 + +0.5 3,643,543 + +0.3 3,643,549 - +1.2 3,643,551 - +0.4 3,643,551 - -0.7 3,643,553 + +0.8 3,643,561 - -0.5 3,643,567 + +0.4 3,643,573 - -1.0 3,643,591 - +0.2 3,643,621 + -0.7 3,643,626 + -0.1 3,643,626 + -0.6 3,643,626 + -0.4 3,643,626 + -0.1 3,643,626 + -0.1 3,643,634 - -2.2 3,643,636 - -0.4 3,643,636 - +0.3 3,643,645 - -1.0 3,643,646 + -1.2 3,643,659 - +0.2 3,643,848 - BWI76_RS17555 0.12 +0.2 3,643,854 + BWI76_RS17555 0.13 -1.0 3,643,933 - BWI76_RS17555 0.18 -0.7 3,643,942 + BWI76_RS17555 0.19 -1.2 3,643,968 + -0.2 3,644,023 + BWI76_RS17555 0.24 -0.3 3,644,084 - BWI76_RS17555 0.28 -2.2 3,644,314 + BWI76_RS17555 0.44 +0.2 3,644,314 + BWI76_RS17555 0.44 +0.7 3,644,324 + BWI76_RS17555 0.45 -0.5 3,644,332 - BWI76_RS17555 0.45 -1.9 3,644,332 - BWI76_RS17555 0.45 -0.8 3,644,339 + BWI76_RS17555 0.46 -0.4 3,644,659 + BWI76_RS17555 0.67 +0.2 3,644,792 + BWI76_RS17555 0.76 -0.7 3,644,826 + BWI76_RS17555 0.79 +0.7 3,644,852 + BWI76_RS17555 0.81 +0.7 3,645,060 + +0.4 3,645,132 - +0.2 3,645,251 + -4.2 3,645,659 - BWI76_RS17560 0.18 -1.0 3,645,950 - BWI76_RS17560 0.31 -1.4
Or see this region's nucleotide sequence