Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15370 and BWI76_RS15375 overlap by 7 nucleotides BWI76_RS15375 and BWI76_RS15380 are separated by 413 nucleotides
BWI76_RS15370: BWI76_RS15370 - hemagglutinin, at 3,218,723 to 3,221,767
_RS15370
BWI76_RS15375: BWI76_RS15375 - peptide transporter, at 3,221,761 to 3,222,375
_RS15375
BWI76_RS15380: BWI76_RS15380 - hypothetical protein, at 3,222,789 to 3,223,277
_RS15380
Position (kb)
3221
3222
3223 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3220.801 kb on + strand, within BWI76_RS15370 at 3220.891 kb on + strand, within BWI76_RS15370 at 3220.939 kb on + strand, within BWI76_RS15370 at 3220.957 kb on + strand, within BWI76_RS15370 at 3221.058 kb on - strand, within BWI76_RS15370 at 3221.065 kb on - strand at 3221.078 kb on - strand, within BWI76_RS15370 at 3221.100 kb on - strand, within BWI76_RS15370 at 3221.175 kb on + strand, within BWI76_RS15370 at 3221.190 kb on - strand, within BWI76_RS15370 at 3221.249 kb on + strand, within BWI76_RS15370 at 3221.264 kb on + strand, within BWI76_RS15370 at 3221.272 kb on + strand, within BWI76_RS15370 at 3221.272 kb on + strand, within BWI76_RS15370 at 3221.272 kb on + strand, within BWI76_RS15370 at 3221.280 kb on - strand, within BWI76_RS15370 at 3221.282 kb on - strand, within BWI76_RS15370 at 3221.295 kb on + strand, within BWI76_RS15370 at 3221.297 kb on + strand, within BWI76_RS15370 at 3221.312 kb on - strand, within BWI76_RS15370 at 3221.312 kb on - strand, within BWI76_RS15370 at 3221.349 kb on - strand, within BWI76_RS15370 at 3221.481 kb on + strand at 3221.481 kb on + strand at 3221.489 kb on - strand at 3221.489 kb on - strand at 3221.489 kb on - strand at 3221.489 kb on - strand at 3221.489 kb on - strand at 3221.489 kb on - strand at 3221.489 kb on - strand at 3221.489 kb on - strand at 3221.489 kb on - strand at 3221.504 kb on + strand at 3221.514 kb on + strand at 3221.519 kb on + strand at 3221.653 kb on + strand at 3221.662 kb on - strand at 3221.662 kb on - strand at 3221.671 kb on + strand at 3221.703 kb on + strand at 3221.708 kb on + strand at 3221.728 kb on + strand at 3221.788 kb on + strand at 3221.817 kb on + strand at 3221.837 kb on - strand, within BWI76_RS15375 at 3221.958 kb on + strand, within BWI76_RS15375 at 3221.963 kb on + strand, within BWI76_RS15375 at 3222.019 kb on + strand, within BWI76_RS15375 at 3222.075 kb on + strand, within BWI76_RS15375 at 3222.075 kb on + strand, within BWI76_RS15375 at 3222.083 kb on - strand, within BWI76_RS15375 at 3222.119 kb on + strand, within BWI76_RS15375 at 3222.301 kb on - strand, within BWI76_RS15375 at 3222.303 kb on - strand, within BWI76_RS15375 at 3222.303 kb on - strand, within BWI76_RS15375 at 3222.303 kb on - strand, within BWI76_RS15375 at 3222.316 kb on - strand at 3222.332 kb on - strand at 3222.535 kb on - strand at 3222.540 kb on - strand at 3222.540 kb on - strand at 3222.540 kb on - strand at 3222.568 kb on + strand at 3222.589 kb on - strand at 3222.705 kb on + strand at 3222.719 kb on + strand at 3222.867 kb on + strand, within BWI76_RS15380 at 3222.874 kb on + strand, within BWI76_RS15380 at 3222.879 kb on + strand, within BWI76_RS15380 at 3222.905 kb on + strand, within BWI76_RS15380 at 3222.949 kb on + strand, within BWI76_RS15380 at 3222.984 kb on + strand, within BWI76_RS15380 at 3223.057 kb on + strand, within BWI76_RS15380 at 3223.099 kb on + strand, within BWI76_RS15380 at 3223.099 kb on + strand, within BWI76_RS15380 at 3223.099 kb on + strand, within BWI76_RS15380 at 3223.099 kb on + strand, within BWI76_RS15380 at 3223.099 kb on + strand, within BWI76_RS15380 at 3223.099 kb on + strand, within BWI76_RS15380 at 3223.099 kb on + strand, within BWI76_RS15380 at 3223.099 kb on + strand, within BWI76_RS15380 at 3223.099 kb on + strand, within BWI76_RS15380 at 3223.099 kb on + strand, within BWI76_RS15380 at 3223.099 kb on + strand, within BWI76_RS15380 at 3223.099 kb on + strand, within BWI76_RS15380 at 3223.107 kb on - strand, within BWI76_RS15380 at 3223.131 kb on + strand, within BWI76_RS15380 at 3223.138 kb on - strand, within BWI76_RS15380 at 3223.195 kb on - strand, within BWI76_RS15380 at 3223.197 kb on + strand at 3223.285 kb on - strand at 3223.287 kb on + strand at 3223.350 kb on + strand at 3223.350 kb on + strand at 3223.350 kb on + strand at 3223.355 kb on + strand at 3223.358 kb on - strand at 3223.358 kb on - strand at 3223.358 kb on - strand at 3223.358 kb on - strand at 3223.369 kb on - strand at 3223.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 3,220,801 + BWI76_RS15370 0.68 -0.2 3,220,891 + BWI76_RS15370 0.71 -0.4 3,220,939 + BWI76_RS15370 0.73 -1.8 3,220,957 + BWI76_RS15370 0.73 +1.1 3,221,058 - BWI76_RS15370 0.77 +0.0 3,221,065 - +0.2 3,221,078 - BWI76_RS15370 0.77 -2.9 3,221,100 - BWI76_RS15370 0.78 +1.4 3,221,175 + BWI76_RS15370 0.81 +0.4 3,221,190 - BWI76_RS15370 0.81 -0.4 3,221,249 + BWI76_RS15370 0.83 -1.1 3,221,264 + BWI76_RS15370 0.83 +1.1 3,221,272 + BWI76_RS15370 0.84 -0.2 3,221,272 + BWI76_RS15370 0.84 -1.1 3,221,272 + BWI76_RS15370 0.84 +1.2 3,221,280 - BWI76_RS15370 0.84 -0.2 3,221,282 - BWI76_RS15370 0.84 +0.6 3,221,295 + BWI76_RS15370 0.84 +1.1 3,221,297 + BWI76_RS15370 0.85 -1.8 3,221,312 - BWI76_RS15370 0.85 -0.6 3,221,312 - BWI76_RS15370 0.85 -0.8 3,221,349 - BWI76_RS15370 0.86 -1.3 3,221,481 + +0.6 3,221,481 + +2.4 3,221,489 - +0.7 3,221,489 - -0.0 3,221,489 - -2.6 3,221,489 - -1.4 3,221,489 - -1.1 3,221,489 - +0.5 3,221,489 - -0.9 3,221,489 - -1.8 3,221,489 - +0.6 3,221,504 + -0.6 3,221,514 + +1.9 3,221,519 + +0.0 3,221,653 + -0.7 3,221,662 - +1.2 3,221,662 - +0.8 3,221,671 + -1.4 3,221,703 + +2.8 3,221,708 + +0.8 3,221,728 + +1.5 3,221,788 + +0.2 3,221,817 + -1.8 3,221,837 - BWI76_RS15375 0.12 -0.4 3,221,958 + BWI76_RS15375 0.32 -0.9 3,221,963 + BWI76_RS15375 0.33 -1.0 3,222,019 + BWI76_RS15375 0.42 -0.4 3,222,075 + BWI76_RS15375 0.51 -0.9 3,222,075 + BWI76_RS15375 0.51 +0.7 3,222,083 - BWI76_RS15375 0.52 +0.1 3,222,119 + BWI76_RS15375 0.58 -0.4 3,222,301 - BWI76_RS15375 0.88 -0.4 3,222,303 - BWI76_RS15375 0.88 -0.6 3,222,303 - BWI76_RS15375 0.88 -1.3 3,222,303 - BWI76_RS15375 0.88 -0.8 3,222,316 - +0.7 3,222,332 - -2.7 3,222,535 - +1.0 3,222,540 - -1.1 3,222,540 - +1.1 3,222,540 - -0.6 3,222,568 + -0.7 3,222,589 - +0.8 3,222,705 + +0.6 3,222,719 + -1.6 3,222,867 + BWI76_RS15380 0.16 +0.3 3,222,874 + BWI76_RS15380 0.17 +0.1 3,222,879 + BWI76_RS15380 0.18 +0.3 3,222,905 + BWI76_RS15380 0.24 +0.0 3,222,949 + BWI76_RS15380 0.33 -0.4 3,222,984 + BWI76_RS15380 0.40 +0.6 3,223,057 + BWI76_RS15380 0.55 -0.5 3,223,099 + BWI76_RS15380 0.63 +0.8 3,223,099 + BWI76_RS15380 0.63 -0.7 3,223,099 + BWI76_RS15380 0.63 +1.4 3,223,099 + BWI76_RS15380 0.63 -2.5 3,223,099 + BWI76_RS15380 0.63 +1.2 3,223,099 + BWI76_RS15380 0.63 -0.5 3,223,099 + BWI76_RS15380 0.63 -1.1 3,223,099 + BWI76_RS15380 0.63 -1.4 3,223,099 + BWI76_RS15380 0.63 +0.1 3,223,099 + BWI76_RS15380 0.63 +1.0 3,223,099 + BWI76_RS15380 0.63 -1.6 3,223,099 + BWI76_RS15380 0.63 +0.1 3,223,107 - BWI76_RS15380 0.65 -0.9 3,223,131 + BWI76_RS15380 0.70 +0.2 3,223,138 - BWI76_RS15380 0.71 -0.1 3,223,195 - BWI76_RS15380 0.83 -3.0 3,223,197 + -0.9 3,223,285 - -1.5 3,223,287 + +0.1 3,223,350 + -3.1 3,223,350 + +0.2 3,223,350 + -0.0 3,223,355 + -0.2 3,223,358 - +0.9 3,223,358 - +2.8 3,223,358 - +1.2 3,223,358 - -0.6 3,223,369 - -0.2 3,223,375 - -0.1
Or see this region's nucleotide sequence