Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14160 and BWI76_RS14165 are separated by 243 nucleotides BWI76_RS14165 and BWI76_RS14170 are separated by 48 nucleotides BWI76_RS14170 and BWI76_RS14175 are separated by 135 nucleotides
BWI76_RS14160: BWI76_RS14160 - ABC transporter substrate-binding protein, at 2,962,550 to 2,963,512
_RS14160
BWI76_RS14165: BWI76_RS14165 - trans-acting regulatory protein of aco operon, at 2,963,756 to 2,966,509
_RS14165
BWI76_RS14170: BWI76_RS14170 - hypothetical protein, at 2,966,558 to 2,966,941
_RS14170
BWI76_RS14175: BWI76_RS14175 - dehydrogenase, at 2,967,077 to 2,967,952
_RS14175
Position (kb)
2963
2964
2965
2966
2967 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2962.964 kb on + strand, within BWI76_RS14160 at 2963.020 kb on - strand, within BWI76_RS14160 at 2963.198 kb on - strand, within BWI76_RS14160 at 2963.241 kb on + strand, within BWI76_RS14160 at 2963.375 kb on + strand, within BWI76_RS14160 at 2963.388 kb on + strand, within BWI76_RS14160 at 2963.481 kb on + strand at 2963.481 kb on + strand at 2963.557 kb on + strand at 2963.826 kb on - strand at 2964.005 kb on - strand at 2964.137 kb on - strand, within BWI76_RS14165 at 2964.246 kb on - strand, within BWI76_RS14165 at 2964.290 kb on - strand, within BWI76_RS14165 at 2964.461 kb on - strand, within BWI76_RS14165 at 2964.696 kb on - strand, within BWI76_RS14165 at 2964.803 kb on - strand, within BWI76_RS14165 at 2965.341 kb on + strand, within BWI76_RS14165 at 2965.341 kb on + strand, within BWI76_RS14165 at 2965.530 kb on - strand at 2965.808 kb on + strand, within BWI76_RS14165 at 2965.813 kb on + strand, within BWI76_RS14165 at 2966.029 kb on + strand, within BWI76_RS14165 at 2966.029 kb on - strand, within BWI76_RS14165 at 2966.341 kb on + strand at 2966.408 kb on - strand at 2966.446 kb on + strand at 2966.561 kb on + strand at 2966.569 kb on - strand at 2966.579 kb on + strand at 2966.656 kb on + strand, within BWI76_RS14170 at 2966.741 kb on - strand, within BWI76_RS14170 at 2966.767 kb on - strand, within BWI76_RS14170 at 2966.840 kb on + strand, within BWI76_RS14170 at 2966.889 kb on - strand, within BWI76_RS14170 at 2966.907 kb on + strand at 2966.979 kb on + strand at 2966.979 kb on + strand at 2967.034 kb on - strand at 2967.036 kb on + strand at 2967.075 kb on + strand at 2967.075 kb on + strand at 2967.123 kb on + strand at 2967.147 kb on - strand at 2967.156 kb on + strand at 2967.220 kb on - strand, within BWI76_RS14175 at 2967.277 kb on + strand at 2967.285 kb on - strand, within BWI76_RS14175 at 2967.335 kb on - strand, within BWI76_RS14175
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 2,962,964 + BWI76_RS14160 0.43 +0.6 2,963,020 - BWI76_RS14160 0.49 -0.3 2,963,198 - BWI76_RS14160 0.67 -0.1 2,963,241 + BWI76_RS14160 0.72 +1.8 2,963,375 + BWI76_RS14160 0.86 +0.4 2,963,388 + BWI76_RS14160 0.87 +0.0 2,963,481 + -1.3 2,963,481 + +0.9 2,963,557 + +0.3 2,963,826 - -1.5 2,964,005 - -0.5 2,964,137 - BWI76_RS14165 0.14 -0.7 2,964,246 - BWI76_RS14165 0.18 -1.4 2,964,290 - BWI76_RS14165 0.19 +0.1 2,964,461 - BWI76_RS14165 0.26 +0.9 2,964,696 - BWI76_RS14165 0.34 +0.4 2,964,803 - BWI76_RS14165 0.38 -1.3 2,965,341 + BWI76_RS14165 0.58 -0.1 2,965,341 + BWI76_RS14165 0.58 -0.3 2,965,530 - +3.9 2,965,808 + BWI76_RS14165 0.75 +0.7 2,965,813 + BWI76_RS14165 0.75 -0.3 2,966,029 + BWI76_RS14165 0.83 -0.3 2,966,029 - BWI76_RS14165 0.83 -0.8 2,966,341 + +0.4 2,966,408 - +1.0 2,966,446 + +0.5 2,966,561 + -0.1 2,966,569 - -0.2 2,966,579 + +1.2 2,966,656 + BWI76_RS14170 0.26 +0.7 2,966,741 - BWI76_RS14170 0.48 +2.2 2,966,767 - BWI76_RS14170 0.54 -0.3 2,966,840 + BWI76_RS14170 0.73 +1.1 2,966,889 - BWI76_RS14170 0.86 -0.8 2,966,907 + +1.4 2,966,979 + +0.1 2,966,979 + -2.3 2,967,034 - +0.0 2,967,036 + +1.6 2,967,075 + -1.0 2,967,075 + -0.6 2,967,123 + +1.3 2,967,147 - -0.2 2,967,156 + -0.3 2,967,220 - BWI76_RS14175 0.16 -0.6 2,967,277 + +0.3 2,967,285 - BWI76_RS14175 0.24 -3.1 2,967,335 - BWI76_RS14175 0.29 -2.3
Or see this region's nucleotide sequence