Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14015 and BWI76_RS14020 are separated by 18 nucleotides BWI76_RS14020 and BWI76_RS14025 are separated by 307 nucleotides
BWI76_RS14015: BWI76_RS14015 - L-2,4-diaminobutyrate decarboxylase, at 2,928,356 to 2,929,828
_RS14015
BWI76_RS14020: BWI76_RS14020 - aspartate aminotransferase family protein, at 2,929,847 to 2,931,232
_RS14020
BWI76_RS14025: BWI76_RS14025 - NMT1/THI5 like domain-containing protein, at 2,931,540 to 2,932,475
_RS14025
Position (kb)
2929
2930
2931
2932 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2929.084 kb on + strand, within BWI76_RS14015 at 2929.119 kb on + strand, within BWI76_RS14015 at 2929.127 kb on - strand, within BWI76_RS14015 at 2929.734 kb on - strand at 2930.517 kb on + strand, within BWI76_RS14020 at 2930.675 kb on - strand, within BWI76_RS14020 at 2931.084 kb on + strand, within BWI76_RS14020 at 2931.084 kb on - strand, within BWI76_RS14020 at 2931.084 kb on - strand, within BWI76_RS14020 at 2931.152 kb on - strand at 2931.185 kb on + strand at 2931.185 kb on + strand at 2931.239 kb on + strand at 2931.239 kb on + strand at 2931.241 kb on + strand at 2931.245 kb on - strand at 2931.247 kb on - strand at 2931.247 kb on - strand at 2931.247 kb on - strand at 2931.249 kb on + strand at 2931.249 kb on + strand at 2931.249 kb on + strand at 2931.257 kb on - strand at 2931.257 kb on - strand at 2931.260 kb on - strand at 2931.265 kb on - strand at 2931.265 kb on - strand at 2931.311 kb on - strand at 2931.320 kb on + strand at 2931.320 kb on + strand at 2931.328 kb on - strand at 2931.377 kb on + strand at 2931.377 kb on + strand at 2931.386 kb on - strand at 2931.533 kb on + strand at 2931.538 kb on + strand at 2931.546 kb on - strand at 2931.546 kb on - strand at 2931.546 kb on - strand at 2931.546 kb on - strand at 2931.588 kb on - strand at 2931.589 kb on + strand at 2931.600 kb on + strand at 2931.601 kb on + strand at 2931.619 kb on - strand at 2931.620 kb on - strand at 2931.639 kb on - strand, within BWI76_RS14025 at 2931.665 kb on + strand, within BWI76_RS14025 at 2931.672 kb on + strand, within BWI76_RS14025 at 2931.703 kb on + strand, within BWI76_RS14025 at 2931.703 kb on + strand, within BWI76_RS14025 at 2931.711 kb on - strand, within BWI76_RS14025 at 2931.734 kb on + strand, within BWI76_RS14025 at 2931.772 kb on - strand, within BWI76_RS14025 at 2931.898 kb on + strand, within BWI76_RS14025 at 2932.013 kb on - strand, within BWI76_RS14025 at 2932.197 kb on + strand, within BWI76_RS14025
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 2,929,084 + BWI76_RS14015 0.49 +0.9 2,929,119 + BWI76_RS14015 0.52 -0.7 2,929,127 - BWI76_RS14015 0.52 +0.0 2,929,734 - +0.3 2,930,517 + BWI76_RS14020 0.48 -0.0 2,930,675 - BWI76_RS14020 0.60 -2.4 2,931,084 + BWI76_RS14020 0.89 -0.3 2,931,084 - BWI76_RS14020 0.89 -2.7 2,931,084 - BWI76_RS14020 0.89 -0.8 2,931,152 - +4.0 2,931,185 + +0.6 2,931,185 + -0.3 2,931,239 + +0.8 2,931,239 + +2.3 2,931,241 + -0.1 2,931,245 - -1.4 2,931,247 - +0.7 2,931,247 - +0.5 2,931,247 - +0.9 2,931,249 + +0.8 2,931,249 + -0.9 2,931,249 + -3.2 2,931,257 - -0.7 2,931,257 - -1.9 2,931,260 - -1.8 2,931,265 - -0.2 2,931,265 - +1.2 2,931,311 - +0.4 2,931,320 + +0.3 2,931,320 + -1.5 2,931,328 - -1.1 2,931,377 + +0.9 2,931,377 + +0.4 2,931,386 - -1.4 2,931,533 + -1.5 2,931,538 + +0.2 2,931,546 - +1.0 2,931,546 - -2.0 2,931,546 - +0.5 2,931,546 - +1.2 2,931,588 - -2.3 2,931,589 + +0.1 2,931,600 + +0.5 2,931,601 + +1.8 2,931,619 - +0.1 2,931,620 - -0.3 2,931,639 - BWI76_RS14025 0.11 -1.6 2,931,665 + BWI76_RS14025 0.13 -0.8 2,931,672 + BWI76_RS14025 0.14 +2.2 2,931,703 + BWI76_RS14025 0.17 +1.0 2,931,703 + BWI76_RS14025 0.17 +2.3 2,931,711 - BWI76_RS14025 0.18 -1.0 2,931,734 + BWI76_RS14025 0.21 +0.2 2,931,772 - BWI76_RS14025 0.25 +0.3 2,931,898 + BWI76_RS14025 0.38 -0.1 2,932,013 - BWI76_RS14025 0.51 +1.4 2,932,197 + BWI76_RS14025 0.70 -1.2
Or see this region's nucleotide sequence