Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13640 and BWI76_RS13645 are separated by 76 nucleotides BWI76_RS13645 and BWI76_RS13650 are separated by 129 nucleotides BWI76_RS13650 and BWI76_RS13655 overlap by 4 nucleotides
BWI76_RS13640: BWI76_RS13640 - binding-protein-dependent transport system inner membrane protein, at 2,842,750 to 2,844,405
_RS13640
BWI76_RS13645: BWI76_RS13645 - N-hydroxyarylamine O-acetyltransferase, at 2,844,482 to 2,845,327
_RS13645
BWI76_RS13650: BWI76_RS13650 - respiratory nitrate reductase subunit gamma, at 2,845,457 to 2,846,137
_RS13650
BWI76_RS13655: BWI76_RS13655 - nitrate reductase molybdenum cofactor assembly chaperone, at 2,846,134 to 2,846,829
_RS13655
Position (kb)
2844
2845
2846 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2843.913 kb on + strand, within BWI76_RS13640 at 2843.990 kb on - strand, within BWI76_RS13640 at 2844.089 kb on - strand, within BWI76_RS13640 at 2844.310 kb on + strand at 2844.531 kb on + strand at 2844.714 kb on - strand, within BWI76_RS13645 at 2844.732 kb on + strand, within BWI76_RS13645 at 2844.986 kb on - strand, within BWI76_RS13645 at 2845.315 kb on - strand at 2845.477 kb on + strand at 2845.479 kb on + strand at 2845.512 kb on + strand at 2845.653 kb on - strand, within BWI76_RS13650 at 2846.090 kb on + strand at 2846.096 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 2,843,913 + BWI76_RS13640 0.70 +0.7 2,843,990 - BWI76_RS13640 0.75 -2.5 2,844,089 - BWI76_RS13640 0.81 -0.4 2,844,310 + -0.2 2,844,531 + +0.4 2,844,714 - BWI76_RS13645 0.27 +0.3 2,844,732 + BWI76_RS13645 0.30 -1.8 2,844,986 - BWI76_RS13645 0.60 -1.9 2,845,315 - -1.0 2,845,477 + +1.2 2,845,479 + -0.2 2,845,512 + +0.9 2,845,653 - BWI76_RS13650 0.29 -0.1 2,846,090 + -2.8 2,846,096 + -1.6
Or see this region's nucleotide sequence