Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12915 and BWI76_RS12920 are separated by 51 nucleotides BWI76_RS12920 and BWI76_RS12925 overlap by 4 nucleotides BWI76_RS12925 and BWI76_RS12930 overlap by 4 nucleotides BWI76_RS12930 and BWI76_RS12935 overlap by 11 nucleotides
BWI76_RS12915: BWI76_RS12915 - 4-hydroxyphenylacetate permease, at 2,690,761 to 2,692,134
_RS12915
BWI76_RS12920: BWI76_RS12920 - DNA-binding protein, at 2,692,186 to 2,692,797
_RS12920
BWI76_RS12925: BWI76_RS12925 - efflux transporter periplasmic adaptor subunit, at 2,692,794 to 2,693,717
_RS12925
BWI76_RS12930: BWI76_RS12930 - DUF1656 domain-containing protein, at 2,693,714 to 2,693,929
_RS12930
BWI76_RS12935: BWI76_RS12935 - fusaric acid resistance protein, at 2,693,919 to 2,695,958
_RS12935
Position (kb)
2692
2693
2694 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2691.858 kb on + strand, within BWI76_RS12915 at 2691.901 kb on - strand, within BWI76_RS12915 at 2692.037 kb on - strand at 2692.460 kb on - strand, within BWI76_RS12920 at 2692.821 kb on + strand at 2692.824 kb on - strand at 2692.996 kb on - strand, within BWI76_RS12925 at 2693.465 kb on + strand, within BWI76_RS12925 at 2693.734 kb on - strand at 2694.051 kb on + strand at 2694.369 kb on - strand, within BWI76_RS12935 at 2694.511 kb on - strand, within BWI76_RS12935 at 2694.527 kb on + strand, within BWI76_RS12935
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 2,691,858 + BWI76_RS12915 0.80 +0.8 2,691,901 - BWI76_RS12915 0.83 +1.5 2,692,037 - +1.1 2,692,460 - BWI76_RS12920 0.45 -2.5 2,692,821 + -1.2 2,692,824 - -1.2 2,692,996 - BWI76_RS12925 0.22 -1.5 2,693,465 + BWI76_RS12925 0.73 -0.1 2,693,734 - +0.4 2,694,051 + -0.3 2,694,369 - BWI76_RS12935 0.22 +0.1 2,694,511 - BWI76_RS12935 0.29 +0.0 2,694,527 + BWI76_RS12935 0.30 -3.5
Or see this region's nucleotide sequence