Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10830 and BWI76_RS10835 are separated by 50 nucleotides BWI76_RS10835 and BWI76_RS10840 are separated by 155 nucleotides BWI76_RS10840 and BWI76_RS10845 are separated by 122 nucleotides BWI76_RS10845 and BWI76_RS10850 overlap by 4 nucleotides
BWI76_RS10830: BWI76_RS10830 - phosphate starvation-inducible protein PhoH, at 2,288,942 to 2,290,006
_RS10830
BWI76_RS10835: BWI76_RS10835 - hypothetical protein, at 2,290,057 to 2,290,350
_RS10835
BWI76_RS10840: BWI76_RS10840 - membrane protein, at 2,290,506 to 2,291,294
_RS10840
BWI76_RS10845: BWI76_RS10845 - DNA-binding response regulator, at 2,291,417 to 2,292,112
_RS10845
BWI76_RS10850: BWI76_RS10850 - two-component sensor histidine kinase, at 2,292,109 to 2,293,305
_RS10850
Position (kb)
2290
2291
2292 Strain fitness (log2 ratio)
-2
-1
0
1 at 2289.537 kb on - strand, within BWI76_RS10830 at 2289.668 kb on + strand, within BWI76_RS10830 at 2289.708 kb on - strand, within BWI76_RS10830 at 2289.997 kb on + strand at 2290.057 kb on + strand at 2290.065 kb on - strand at 2290.082 kb on - strand at 2290.159 kb on + strand, within BWI76_RS10835 at 2290.566 kb on + strand at 2290.618 kb on - strand, within BWI76_RS10840 at 2290.642 kb on + strand, within BWI76_RS10840 at 2290.642 kb on + strand, within BWI76_RS10840 at 2290.652 kb on + strand, within BWI76_RS10840 at 2290.846 kb on + strand, within BWI76_RS10840 at 2291.271 kb on - strand at 2291.536 kb on + strand, within BWI76_RS10845 at 2291.544 kb on - strand, within BWI76_RS10845 at 2291.617 kb on - strand, within BWI76_RS10845 at 2291.719 kb on + strand, within BWI76_RS10845 at 2291.720 kb on + strand, within BWI76_RS10845 at 2292.001 kb on - strand, within BWI76_RS10845 at 2292.097 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 2,289,537 - BWI76_RS10830 0.56 +0.2 2,289,668 + BWI76_RS10830 0.68 +0.3 2,289,708 - BWI76_RS10830 0.72 -1.2 2,289,997 + -0.2 2,290,057 + -0.6 2,290,065 - -0.2 2,290,082 - -1.7 2,290,159 + BWI76_RS10835 0.35 -2.0 2,290,566 + -0.6 2,290,618 - BWI76_RS10840 0.14 -0.2 2,290,642 + BWI76_RS10840 0.17 -1.7 2,290,642 + BWI76_RS10840 0.17 -0.8 2,290,652 + BWI76_RS10840 0.19 -2.5 2,290,846 + BWI76_RS10840 0.43 -1.5 2,291,271 - -1.0 2,291,536 + BWI76_RS10845 0.17 +0.8 2,291,544 - BWI76_RS10845 0.18 -0.3 2,291,617 - BWI76_RS10845 0.29 -2.0 2,291,719 + BWI76_RS10845 0.43 +1.4 2,291,720 + BWI76_RS10845 0.44 +0.0 2,292,001 - BWI76_RS10845 0.84 +0.7 2,292,097 - +0.8
Or see this region's nucleotide sequence