Experiment: K. M5a1 KLEB23 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10505 and BWI76_RS10510 overlap by 23 nucleotides BWI76_RS10510 and BWI76_RS10515 overlap by 4 nucleotides BWI76_RS10515 and BWI76_RS10520 are separated by 41 nucleotides BWI76_RS10520 and BWI76_RS10525 are separated by 109 nucleotides
BWI76_RS10505: BWI76_RS10505 - putative guanine/xanthin permease, at 2,220,168 to 2,221,550
_RS10505
BWI76_RS10510: BWI76_RS10510 - OHCU decarboxylase, at 2,221,528 to 2,222,028
_RS10510
BWI76_RS10515: BWI76_RS10515 - 5-hydroxyisourate hydrolase, at 2,222,025 to 2,222,351
_RS10515
BWI76_RS10520: BWI76_RS10520 - guanine deaminase, at 2,222,393 to 2,223,712
_RS10520
BWI76_RS10525: BWI76_RS10525 - cytosine/purines uracil thiamine allantoin permease, at 2,223,822 to 2,225,309
_RS10525
Position (kb)
2222
2223
2224 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2221.796 kb on + strand, within BWI76_RS10510 at 2221.814 kb on - strand, within BWI76_RS10510 at 2221.970 kb on + strand, within BWI76_RS10510 at 2222.383 kb on + strand at 2222.483 kb on + strand at 2222.639 kb on + strand, within BWI76_RS10520 at 2222.647 kb on - strand, within BWI76_RS10520 at 2222.647 kb on - strand, within BWI76_RS10520 at 2222.711 kb on - strand, within BWI76_RS10520 at 2222.874 kb on + strand, within BWI76_RS10520 at 2222.973 kb on + strand, within BWI76_RS10520 at 2223.258 kb on + strand, within BWI76_RS10520 at 2223.411 kb on - strand, within BWI76_RS10520 at 2223.419 kb on - strand, within BWI76_RS10520 at 2223.564 kb on + strand, within BWI76_RS10520 at 2223.661 kb on - strand at 2223.998 kb on - strand, within BWI76_RS10525 at 2224.400 kb on + strand, within BWI76_RS10525 at 2224.465 kb on - strand, within BWI76_RS10525 at 2224.654 kb on - strand, within BWI76_RS10525
Per-strain Table
Position Strand Gene LocusTag Fraction K. M5a1 KLEB23 MOI 1 remove 2,221,796 + BWI76_RS10510 0.53 +0.7 2,221,814 - BWI76_RS10510 0.57 +1.2 2,221,970 + BWI76_RS10510 0.88 -0.6 2,222,383 + +0.3 2,222,483 + -1.3 2,222,639 + BWI76_RS10520 0.19 -0.4 2,222,647 - BWI76_RS10520 0.19 -1.8 2,222,647 - BWI76_RS10520 0.19 -0.3 2,222,711 - BWI76_RS10520 0.24 +1.0 2,222,874 + BWI76_RS10520 0.36 +0.5 2,222,973 + BWI76_RS10520 0.44 +2.0 2,223,258 + BWI76_RS10520 0.66 +0.4 2,223,411 - BWI76_RS10520 0.77 -0.6 2,223,419 - BWI76_RS10520 0.78 -1.9 2,223,564 + BWI76_RS10520 0.89 -0.9 2,223,661 - -0.3 2,223,998 - BWI76_RS10525 0.12 -0.7 2,224,400 + BWI76_RS10525 0.39 -1.8 2,224,465 - BWI76_RS10525 0.43 -0.0 2,224,654 - BWI76_RS10525 0.56 +0.6
Or see this region's nucleotide sequence